Leica - ARTOS 3D Array Tomography Solution

เครื่องตัดชิ้นตัวอย่างแบบบางพิเศษแบบต่อเนื่องเพื่อสร้างภาพสามมิติ ยี่ห้อ ไลก้า รุ่น Leica ARTOS 3D

Serial Sections ที่มีคุณภาพ สำหรับ Array Tomography จาก Leica ARTOS 3D

ultrathin serial sections สำหรับ array tomography ที่ใช้เวลาไม่มากโดย ARTOS 3D ultramicrotome

ARTOS 3D (ARray TOmography Solution) สามารถสร้างและเก็บ serial-section เป็นแบบริบบอนได้ถึง 100 section ซึ่งทั้งหมดจะใช้สำหรับ array tomography ด้วย scanning electron microscope (SEM) ซึ่งมันช่วยประหยัดเวลาและได้ผลลัพธ์ที่ดีสำหรับการเตรียมตัวอย่างในงานชีววิทยา และการจัดเตรียมกระบวนการของ SEM คุณจะสามารถเก็บรปภาพได้เร็วและตอบคำถามงานวิจัยได้ดี

Pre-program สำหรับ ARTOS 3D สามารถสร้าง section ribbons ได้มากกว่า 100 section ด้วยขนาดหน้าบล๊อกที่หลากหลาย (µm to mm)
หลักเลี่ยง tricky ต่างๆ เช่น เวลาที่เสียไปกับการเก็บริบบอนเองด้วยมือ โดยใช้ ARTOS 3D ที่สามารถเก็บรินบอนให้ได้อัตโนมัติ
ประหยัดเวลาจากการตั้งค่า SEM โดยการใส่ตัวอย่างๆ ยาว หลายๆชิ้น ซึ่งสามารถใช้ที่เก็บตัวอย่างได้เยอะเพียงชิ้นดียวใส่เข้าไปที่ SEM
มีการทำงานที่ราบรื่นขณะที่มีการย้าย section

ได้ section ที่มองเห็นได้ชัดเจน ARTOS 3D ซึ่งเป็นตัวช่วยแก้ปัญหาสำหรับ correlative light and electron microscopy (CLEM) ที่ใช้กับงานวิจัยโดยเฉพาะ

Get consistent, ultrathin serial sections for array tomography in less time with the ARTOS 3D ultramicrotome.

The ARTOS 3D (ARray TOmography Solution) automatically creates and collects hundreds of serial-section ribbons all ready for array tomography with your scanning electron microscope (SEM). Save time and effort in biological sample preparation and SEM setup procedure so you can quickly get the images you need to answer critical research questions.

Pre-program the ARTOS 3D to produce automatically hundreds of ultrathin section ribbons with flexible block-face sizes (µm to mm)
Avoid tricky, time-consuming manual ribbon collection with the ARTOS 3D integrated collection of fully aligned ribbons
Save SEM setup time by loading several carriers with high section density simultaneously
Smoothly transfer the section carrier through the whole specimen preparation process for a streamlined workflow

As transparent section carriers are available, the ARTOS 3D is also an ideal solution for correlative light and electron microscopy (CLEM).

For research use only.

Faster Array Tomography

How does the ARTOS 3D ultramicrotome allow you to save time when making high quality sections for array tomography?

Array tomography (AT) is a 3D image reconstruction technique for high resolution, quantitative analysis of biological structures. For optimal results, ultrathin, ordered sections are a pre-requisite.

Until now, AT has involved several time-consuming and cumbersome manual steps. The ARTOS 3D solution speeds up the process by automating specimen sectioning and minimizing the time required to align the sections for SEM imaging.

Read on to find out how the ARTOS 3D helps you speed up the workflow while also achieving high quality results.

Fast Sectioning & Easy Alignment for Imaging ARTOS 3D vs. Conventional Ultramicrotome

Less Work for You with the ARTOS 3D Workflow

To obtain excellent results with array tomography in a timely manner, it is crucial to create ultrathin, consistent, and correctly ordered ribbons efficiently. To achieve this advantage, the ARTOS 3D ultramicrotome automates the process of making high quality, ultrathin (>20 nm) sections. The automation eliminates several of the manual work steps required by more conventional ultramicrotomes, such as the EM UC7 also from Leica Microsystems. This automation leads to a large time-savings for serial sectioning and ribbon alignment before SEM imaging.

The manual work steps required with conventional ultramicrotomes are minimized or eliminated by the ARTOS 3D system. There is:

No manually aligning nor positioning of the ribbon sections with an eyelash
No manual handling of the ribbons multiple times for repeat sectioning which increases greatly the risk of wrinkling
No need to perform over and over the same tedious manual process to get wrinkle-free ribbons on the section carrier

Spend less time preparing sections with the automated serial sectioning of the ARTOS 3D. The custom-designed diamond knife enables a seamless transition from one wrinkle-free ribbon to the next with no thickness variation.

Save Time Making High Quality Sections with a Seamless Workflow

Fast section preparation with much less effort

With the ARTOS 3D ultramicrotome, you have a seamless workflow through the whole preparation process by eliminating manual work steps:

Fast setup with programs pre-defined by the user for different section carriers
Automated creation and collection of hundreds of serial sections with minimal user intervention
No more time-consuming and fiddly manual collection involving sorting and positioning of ribbons to attain proper alignment for SEM imaging

Fast setup for serial sectioning is possible with the ARTOS 3D using user-pre-defined programs. A smooth transfer from sectioning to imaging is achieved with a small section carrier used throughout the entire workflow.

Reproducible, Artifact-Free Sections

Consistently high quality sections

The ARTOS 3D ultramicrotome is able to deliver reproducible, high quality sections rapidly by:

Avoiding the artifact-causing conditions of manual sectioning and manipulation due to integrated direct section-ribbon collection
Collecting ultrathin section ribbons without wrinkling by easily adjusting the water flow with the front valve
Minimizing variation in section thickness by eliminating air turbulence and vibration
thanks to a specially-designed draft shield and active damping plate
Sectioning ribbons precisely with the custom-designed 4 mm diamond knife, adaptable to different section carriers
Application: 3D Image Reconstruction of Lymph Nodes
Precise section preparation with the ARTOS 3D enables optimal results with array tomography
In this application, mouse popliteal lymph nodes have been reconstructed with array tomography using serial sections produced by the ARTOS 3D solution. Array tomography enables precise examination of the cellular and protein structures of the lymph nodes, which is critical for various types of immune and cancer studies.
Learn more about how the ARTOS 3D helped optimize this array tomography application in the following report.
Download the Application Note
In addition, the consistent, reproducible sections of the ARTOS 3D ultramicrotome are also ideal for CLEM applications.

Video showing the serial sectioning of mouse lymph nodes with the ARTOS 3D and the generation of 3D images using array tomography. 3D images courtesy: Frank Assen, Ludek Lovicar, Vanessa Zheden, and Michael Sixt, IST Austria, Klosterneuburg.