ibidi µ‑Plate 12 Well
เพลตเพาะเลี้ยงเซลล์แบบ 12 หลุมสีดำ สำหรับการถ่ายภาพเซลล์มีชีวิตและกล้องจุลทรรศน์ความละเอียดสูง
- ก้นหลุมแบบ #1.5 ibidi Polymer Coverslip หรือ #1.5H Glass Coverslip ช่วยให้สามารถถ่ายภาพด้วยกล้องจุลทรรศน์ความละเอียดสูงได้โดยตรง โดยให้คุณภาพทางแสงที่ยอดเยี่ยม
- ประกอบด้วย 12 หลุมอิสระ ในมาตรฐาน ANSI/SLAS (SBS) format รองรับการเพาะเลี้ยงเซลล์และการถ่ายภาพหลายสภาวะพร้อมกัน ช่วยเพิ่มประสิทธิภาพในการทดลองแบบขนาน
- ผนังหลุมสีดำช่วยลดการรบกวนของสัญญาณฟลูออเรสเซนซ์ระหว่างหลุม (Well-to-Well Crosstalk) เพิ่มความแม่นยำในการวิเคราะห์ภาพฟลูออเรสเซนซ์
จุดเด่น
- เหมาะสำหรับงาน Live Cell Imaging และ High-Resolution Microscopy
- รองรับการถ่ายภาพเซลล์โดยตรงผ่านก้นหลุมคุณภาพสูง
- ช่วยให้ดำเนินการทดลองหลายเงื่อนไขพร้อมกันได้อย่างสะดวก
- ลดสัญญาณรบกวนระหว่างหลุม เหมาะสำหรับงาน Fluorescence Microscopy และ High-Content Imaging
- ออกแบบตามมาตรฐานอุตสาหกรรม รองรับระบบวิเคราะห์และเครื่องมืออัตโนมัติได้อย่างมีประสิทธิภาพ
Black 12 well cell culture plate for live cell imaging and high-resolution microscopy
- #1.5 ibidi Polymer Coverslip or #1.5H Glass Coverslip Bottom for direct high-resolution microscopy
- 12 independent wells in ANSI/SLAS (SBS) format for parallel cell culture and imaging workflows
- Black walls reduce well-to-well crosstalk during fluorescence microscopy
12 Well Plate for Cell Culture and High-Resolution Microscopy
The µ-Plate 12 Well is a black 12 well plate for cell culture and high-resolution microscopy. Each well provides a volume of 3 ml and a growth area of 3.6 cm², with a 21.5 mm diameter and an optically clear #1.5 ibidi Polymer Coverslip or #1.5H Glass Coverslip Bottom. The plate supports parallel cell culture, immunofluorescence, live cell imaging, transfection assays, and automated image-based analysis in an ANSI/SLAS format.
Parallel Cell Culture in 12 Round Wells
The twelve independent wells are arranged in a standard A–C and 1–4 layout. Each well provides a culture area and an integrated coverslip bottom for direct microscopy without transferring cells to separate coverslips. The plate format supports parallel comparison of treatments, controls, staining conditions, and biological replicates.
Immunofluorescence and Protein Localization
The µ-Plate 12 Well supports immunofluorescence workflows directly in the plate. Cell cultivation, fixation, staining, washing, and microscopy can be performed in the same wells. The coverslip bottom provides direct optical access for fluorescence imaging, while the 12 well format enables parallel comparison of antibodies, treatments, staining conditions, and biological replicates.
Widefield fluorescence image of Rat1 (rat fibroblast cells) cultivated in the µ-Plate 12 Well ibiTreat. After fixation, the cells were stained with phalloidin-Alexa488 (actin, green) and DAPI (nuclei, blue). The image was acquired on a Nikon microscope with a 20x (left) and a 60x (right) objective lens.
Workflow for cell cultivation, immunofluorescence staining, and imaging in the µ-Plate 12 Well.
Live Cell Imaging and Time-Lapse Studies
Live cell imaging in the µ-Plate 12 Well enables longitudinal observation of cell morphology, proliferation, reporter expression, and cellular responses across multiple experimental conditions. The thin coverslip bottom supports direct imaging on inverted microscopes using brightfield, phase contrast, widefield fluorescence, and confocal microscopy. A compatible ibidi Stage Top Incubator provides temperature, CO2, and humidity control for long-term time-lapse experiments.
Cell-Based Assays and Automated Imaging
The ANSI/SLAS plate format supports integration into compatible automated microscopy and liquid-handling workflows. The 12 well format provides sufficient culture area for parallel cell-based assays, including morphology and proliferation analysis, transfection assays, cytotoxicity assays, and treatment comparisons. Automated high-content screening and image-based analysis can be combined with fluorescence scanners and compatible plate readers. The #1.5H Glass Coverslip Bottom is particularly suited for glass-dependent techniques such as TIRF and super-resolution microscopy.
Specifications
| Outer dimensions (l × w) | 127.8 × 85.5 mm |
| Number of wells | 12 |
| Height with/without lid | 22.4 / 20.0 mm |
| Well-to-well distance | 25.5 mm |
| Single well diameter | 21.5 mm |
| Single well depth | 19.0 mm |
| Volume per well | 3 ml |
| Growth area per well | 3.6 cm² |
| Coating area per well using 3 ml | 9.2 cm² |
| Bottom material | #1.5 ibidi Polymer Coverslip (different surfaces available) #1.5H Glass Coverslip (uncoated and untreated) |
For technical drawings and details, please see:
Instructions µ-Plate 12 Well (PDF)
Instructions µ-Plate 12 Well Glass Bottom (PDF)
Technical Features
Precision, Quality, and Biocompatibility
- µ-Plate (microtiter plate) in ANSI/SLAS (SBS) format
- Ready-to-use for lab automation and high-throughput with robotics, fluorescence scanners, and plate readers
- Twelve round wells with standard numbering (letters A–C and numbers 1–4)
- Excellent inner well flatness and whole plate flatness
- #1.5 ibidi Polymer Coverslip or #1.5H Glass Coverslip bottom with excellent optical quality
- No autofluorescence due to black walls
- Compatible with staining and fixation solutions
- Compatible with immersion oil
- Made from non-cytotoxic, biocompatible materials
- Sterile with single packaging
Product Versions
- With #1.5 ibidi Polymer Coverslip (different surfaces available) or #1.5H Glass Coverslip Bottom
How Does the µ-Plate 12 Well Work?
The µ-Plate 12 Well combines twelve independent wells with a permanently integrated, thin #1.5 ibidi Polymer Coverslip or #1.5H Glass Coverslip Bottom. Cells are cultured and imaged on the same optical surface, allowing direct microscopy from below without transferring samples to separate coverslips.
The ibiTreat surface is tissue culture-treated to support adhesion and growth of many adherent cell types. The Untreated Polymer version provides a hydrophobic surface that can be coated according to the requirements of the cell model. The Glass Bottom version provides a high-precision glass imaging surface for microscopy workflows requiring glass.
µ-Plate 12 Well at a Glance
| Product type | Black 12 well cell culture and imaging microplate |
| Best suited for | Parallel cell culture, immunofluorescence, live cell imaging, transfection assays, and automated imaging |
| Well geometry | 12 round wells arranged A–C and 1–4; 21.5 mm diameter per well; flat coverslip bottom |
| Bottom and surface options | #1.5 ibidi Polymer Coverslip with ibiTreat (81226) or Untreated (81221) surface; #1.5H Glass Coverslip Bottom (81227) |
| Volume and growth area per well | 3 ml; 3.6 cm² |
| Format | ANSI/SLAS (SBS) format; 25.5 mm well spacing |
The ibidi µ-Plate Range
 |  |  |  |  |  |  | |
| 6 Well | 12 Well | 24 Well | 96 Well Round | 96 Well Square | 384 Well | 384 Well Clear | |
| Bottom |   | | | | | | |
| Volume / well | 5 ml | 3 ml | 1 ml | 200 µl | 300 µl | 50 µl | 50 µl |
| Growth area / well | 9.1 cm² | 3.6 cm² | 1.54 cm² | 0.3 cm² | 0.56 cm² | 0.11 cm² | 0.11 cm² |
The ibidi µ-Plates are specifically designed for cell culture, immunofluorescence, and high-end microscopy. They offer flexibility in well size, well volume, and bottom materials. The range includes formats from 6 to 384 wells. All ibidi multiwell plates are user-friendly and ideal for multiplexing and high-throughput applications, providing high optical quality and compatibility with robotic systems. Most µ-Plates are available with either a #1.5 ibidi Polymer Coverslip or #1.5H Glass Coverslip bottom.
Packaging Information
| Cat. No. | Packaging Unit | Packaging Dimensions (l × w × h) | Shipping Weight |
|---|---|---|---|
| 81221, 81226, 81227 | 15 (indiv. packed) | 301 × 173 × 151 mm | 1.2 kg |
| 81221-90, 81226-90, 81227-90 | 90 (indiv. packed) | 505 × 319 × 244 mm | 7.2 kg |
