ชุดบ่มเพาะเซลล์ด้วยก๊าซคาร์บอนไดซ์ออกไซด์และก๊าซออกซิเจนชนิดวางบนแท่นวางตัวอย่างกล้องจุลทรรศน์ รองรับการทำงานกับ Multiwell plate

A stage top incubator for high-throughput live cell imaging on every inverted microscope, with precise temperature regulation, active humidity control, and CO2/O2 regulation

System Components

Applications

The ibidi Stage Top Incubators – Silver Line are ideal for live cell imaging experiments that require physiologic conditions on the microscope.

Combining the ibidi Gas Incubation System and the ibidi Heating System, they precisely control essential parameters such as temperature, humidity, and CO2/O2 levels on the microscope to get reproducible results of, for example:

The ibidi Stage Top Incubators – Silver Line are optimized for high-resolution imaging applications that require extra focus stability, for example:

Solutions for 1 Slide or DishWith the ibidi Stage Top Incubation System Slide/Dish – Silver Line, we also offer low-throughput live cell microscopy solutions.

Specifications

Technical Features

The Principle of the ibidi Stage Top Incubators – Silver Line

Cells react sensitively to changes in their environment. Factors such as temperature, humidity/evaporation, and CO2/O2 levels significantly influence the outcome of cell culture assays. Therefore, it is crucial to maintain optimal conditions on the microscope stage during live cell imaging experiments to achieve biologically relevant and reproducible results.

The ibidi Stage Top Incubators – Silver Line combine the ibidi Heating System – Silver Line and the ibidi Gas Incubation System – Silver Line.

The ibidi Heating System creates stable and homogenous temperatures for high-resolution live cell microscopy. It fits into inverted microscope stages with a universal mounting frame for multiwell plates. When placed in the ibidi Heated Chamber, the cell culture vessels are kept at a stable, defined temperature during the live cell imaging experiment on the microscope.

The ibidi Gas Incubation System provides humid and CO2-rich air for stage-top incubators like the ibidi Heating System. The gas mixture is continuously flushed through the stage top incubator, ensuring maximum humidity and an optimal pH for bicarbonate-buffered liquids.

For hypoxia assays, the ibidi Stage Top Incubators, CO2/O2 – Silver Line allow for the precise control of the oxygen levels in your cell culture vessel. The ambient O2 level can be controlled within a range of 1%–21%, and easily adapted to the experimental requirements.

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Preventing Evaporation With ibidi’s Patented Humidity Control

LCI_evaporation_1.gif

The ratio of dry gas and humidified gas is determined by the actual and preset humidity, and controlled by a feedback mechanism. In a steady-state system (e.g., when both the set and the actual RH are 90%), a mixture of humidified and dry gas enters the ibidi Stage Top Incubator.


Cell behavior is strongly influenced by changes in medium components, such as salts and nutrients. Any evaporation from the cell culture vessels increases the substance concentration in the medium in an undefined way. Therefore, creating ideal humidity conditions—especially during live cell imaging—helps avoid evaporation and leads to reproducible experimental conditions.

The ibidi Stage Top Incubators – Silver Line use the patent-protected ibidi Humidity Control. The unique technology actively humidifies the gas mixture in a fast and reliable way before it enters the stage top incubator. The ibidi Humidity Control allows for very high humidity values of around “real” 90–95%, which provides the most reproducible environmental conditions for cells. Further, in contrast to other systems, the humidity sensor is located close to the sample, which makes the measurement far more transferable to the direct sample/cell environment.

By using the absolute humidity rather than the relative humidity for the feedback control, the ibidi Stage Top Incubators – Silver Line enable an even more precise regulation of the relative humidity (RH) inside the Incubation Chamber. Since the relative humidity is strongly temperature-dependent, determining the absolute humidity allows to compensate for any temperature differences between the humidity sensor and the sample.

Using the ibidi Humidity Control ensures a constant and very high relative humidity (RH) level inside the Incubation Chamber—identical to the conditions in standard cell culture incubators. This reliably prevents evaporation, which is one of the keys to artifact-free results in live cell imaging.

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What Makes the ibidi Humidity Control Special?

Brilliant Images Without Condensation

The temperature of the Heated Lid of the ibidi Stage Top Incubators – Silver Line is independently controlled and optimally distributed, thereby solving the frequently occurring problem of condensation during live cell imaging.

A vertical temperature gradient is created by heating the lid to a temperature higher than the plate (e.g., 40°C). This gradient and the active humidity control prevent condensation on the lid of the cell culture vessel, which is crucial for non-disturbed high-quality microscopy images. The Heated Plate maintains the sample temperature at a constant 37 °C.

Stable and Homogeneous Temperature

The ibidi Stage Top Incubators – Silver Line ensure a precisely controlled, stable temperature in the Incubation Chamber with homogeneous distribution across the wells of the cell culture vessel. The temperature is controlled via continuous current, which reliably prevents position fluctuations and makes the system optimal for high-resolution live cell microscopy with a stable focus.

Stable and consistent temperature distribution in the µ-Plate 96 Well Black during the incubation in the ibidi Heating System Multiwell Plate – Silver Line. Images were acquired with a FLIR thermal camera.

Oxygen Depletion / Hypoxia

The oxygen (O2) concentration at normal atmospheric pressure is around 20–21%. However, the O2 levels in healthy body tissues and our blood are much lower. Variations in the oxygen concentration are part of the normal body system, ranging from 14% in the alveoli to 5% in peripheral tissues.

The oxygen levels can be even lower in pathological situations (hypoxia). Hypoxia especially occurs in tumor tissue, which is characterized by markedly low O2 levels that range from approximately 4% to a minimum of below 0.5%.

The ibidi Stage Top Incubators, CO2/O2 – Silver Line allow for the precise control of oxygen levels in the incubation chamber between the range of 1% to 21%, creating an ideal solution for live cell imaging experiments under hypoxic conditions.


McKeown SR (2014) Defining normoxia, physoxia and hypoxia in tumours-implications for treatment response. Br J Radiol 87(1035):20130676. 10.1259/bjr.20130676.
Read article

Oxygen_in_tissue.jpg

O2 partial pressure in several tissue types (1 kPa ≙ 1 % in gas mix ≙ 7.5 mm Hg).

The Components of the ibidi Stage Top Incubator Multiwell Plate, CO2/O2 – Silver Line

ibidi Heating System Multiwell Plate – Silver LineEasy installation on inverted microscopes that have a K-frame fitting (160 mm x 110 mm)Highest focus stability and xy-stability during microscopyBrilliant images without disturbing condensation on the lid due to optimal heat distributionCompatible with multiwell plates with an ANSI/SLAS (SBS) standard format (85.5 x 127.5 mm2) including the ibidi µ-Platesibidi Gas Incubation System, CO2/O2 – Silver LinePreventing evaporation: constant, very high relative humidity (RH) level inside the stage top incubator due to patented, feedback-controlled humidity regulationPrecise and reliable gas incubation for CO2 and O2