ibidi : Heating System, Multi-Well Plates, K-Frame
ระบบทำความร้อนสำหรับ K-Frame Stages ซึ่งจับ plates multiwell (สำหรับกำลังขยายต่ำ)
- เครื่องอบแบบ stage top ใช้ควบคุมอุณหภูมิของตัวอย่างที่เก็บไว้ใน multi-well plates
- ให้แสงสว่างกับแท่นวางตัวอย่างได้อย่างดี ไม่มีการควบแน่นเนื่องจากฝาปิดที่ให้ความร้อน และเหมาะสำหรับ DIC
- เหมาะสำหรับการใช้งานสร้างภาพเซลล์ – ใช้งานเครื่องอบได้เต็มที่ด้วยกล้องจุลทรรศน์
- ระบบประกอบด้วย : ibidi ตัวควบคุมอุณหภูมิ , แผ่นทำความร้อนสำหรับMulti-Well บน K-Frame Stage (160 มม. x 110 มม.), ฝาปิดที่ให้ความร้อน
การใช้งาน
การถ่ายภาพเซลล์ที่มีชีวิต ซึ่งต้องการสภาวะการบ่มเพาะ บนกล้องจุลทรรศน์ เช่น
- การตรวจ Chemotaxis
- การสร้างเส้นเลือด
- การย้ายเซลล์ การเพิ่มจำนวน การสมาน
- การเพาะเลี้ยงเซลล์ under flow
การใช้งานถ่ายภาพที่มีความเสถียรและโฟกัสเป็นพิเศษ เช่น:
- TIRF
- กล้องจุลทรรศน์คอนโฟคอล
สภาวะไร้อากาศหรือความหนาแน่นของออกซิเดชัน
Heating system for K-Frame Stages which hold multiwell plates (for low magnification only)
- Stage top incubator that permits the user to control the temperature of samples held in multiwell plates
- Excellent illumination of the sample – no condensation due to heated top plate, and suitable for DIC
- Ideal for live cell imaging applications – full incubator conditions on the microscope
- System includes: ibidi Temperature Controller – Blue Line, Heated Plate for multiwell format on K-Frame Stage (160 mm x 110 mm), Heated Lid
System includes:
 ibidi Temperature Controller – Blue Line |  Heated Plate with Heated Glass Bottom |  Heated Glass Lid, Multiwell Plates |
Applications
Live cell imaging that requires incubator conditions on the microscope, for example:
– Angiogenesis
– Cell migration, proliferation, wound healing
– Cell culture under flow
– Confocal microscopy
– Anaerobic conditions or oxidative stress
Rat fibroblasts (cell line)
Find Out More
Please find more detailed information about the planning, conduction, and data analysis of live cell imaging assays here.
Download the whole “Live Cell Imaging” Application Guide as a PDF here.
Specifications ibidi Heating Systems, Multiwell Plates – Blue Line
Please Note:
These Heating Systems only fit K-Frame Stages. For versions to fit other microscopy stages, please contact ibidi directly.
| Temperature Controller – Blue Line | |
| Dimensions | 90 mm x 170 mm x 230 mm |
| Weight | 1720 g / 3.8 lbs |
| Operating conditions | 18-30°C / 64-86°F (min 5°C / 9°F less than set temperature) Indoor use only |
| Input voltage | 230 V / 50 Hz | 115 V / 60 Hz |
| Heated Chamber | |
| Temperature control range | Ambient temperature (min. 18°C) to +45°C |
| K-Frame fitting | 160 mm × 110 mm |
| Observation area | 107.5 mm × 72.5 mm |
| Working distance condenser | ≥ 26 mm |
| Dimensions | 160 mm × 130 mm x 30 mm |
| Weight | 430 g/0.95 lbs |
| Gas inlet | Female Luer connector with Luer Lock |
| Restrictions for Objective Lenses | > 1 mm working distance for coverslip bottom plates, e.g., ibidi µ-Plate 96 Well > 2 mm working distance for thick bottom plates |
Compatibility to Microscopes
| K-Frame Stages (160 mm x 110 mm) | |
| Nikon | Ti-S-E and Ti-S-ER Stages with ibidi Stage Adapter |
| ASI | MS 2000 Series PZ 2000 Series |
| ITK | LMT Series |
| Leica | K-Frame Opening (160 mm x 110 mm) |
| LUDL | BioPrecision 2 Inverted Biopoint 2 Inverted |
| Märzhäuser | SCAN IM Series (except SCAN IM 50 x 50) |
| Olympus | K-Frame Opening (160 mm x 110 mm) |
| PI (Physik Instrumente) | U-780 PILine M-687.XX (from 2012 on) |
| PRIOR | H117 |
| Zeiss | Scanning Stage 130 x 85 mot. P K-Frame Opening (160 mm x 110 mm) |
The ibidi Heating Systems, Multiwell Plates, only require a > 1 mm working distance for multiwell plates with coverslip bottom, e.g., ibidi µ-Plate 96 Well.
Components of the ibidi Heating & Incubation Systems, Multwiell Plates – Blue Line
ibidi Temperature Controller – Blue Line
The Temperature Controller controls the temperature of the different heated components (Heated Lid, Heated Plate, and Heated Glass Bottom). Four control channels are available.
Technical Features:
- Temperature stability: ± 0.2 °C
- Temperature uniformity across the insert: ± 2 °C (for Heated Glass Plate of ibidi Heating System, Multiwell Plates, K-Frame – Blue Line)
- Temperature range: Room temperature – 45 °C
- Control channels: 4
- Includes IncuControl software for instrument control and data recording via a notebook
- External temperature sensor included
- Adjustable alarm function included
- Analog output for extreme noise reduction optional
Heated Plate with Heated Glass Bottom, Multiwell Plates
- To be used with K-Frame-Stages or in combination with the ibidi Stage Adapter (10310) for Nikon Ti-S-E and Ti-S-ER Motorized Stage to K-Frame
- For low magnification only (working distance > 1mm)
- Suitable for DIC
- For use with multiwell plates
Heated Glass Lid, Multiwell Plates
- For use with CO2, O2, and humidity to provide a complete environmental chamber
- Independently controlled glass lid prevents the formation of condensed water on the lid of the microscopy chamber or plate
- No scattering of light or shadow formation
Optional: µ-Dish Microscopy Rack
- A microscopy rack for perfect µ-Dish stabilization
- Adaptable with the ibidi Heating System, Multiwell Plates
- Compatible with all inverted microscopes
- Lids fixed with magnetic positioning for additional stabilization
Optional: µ-Slide Microscopy Rack
- A microscopy rack for perfect µ-Slide stabilization
- Adaptable with the ibidi Heating System, Multiwell Plates
- Compatible with all inverted microscopes
- Lids fixed with magnetic positioning for additional stabilization
Gas Mixer for the Incubation Unit
The heated lid also comes with a gas inlet. The ibidi Gas Incubation System upgrades the ibidi Heating System to a complete stage top incubator for all live cell imaging applications, including high resolution and confocal microscopy.
The ibidi Gas Incubation System is suitable for various experimental conditions (e.g., pH or hypoxia). It is available in two versions: Gas Mixer for CO2, Gas Mixer for CO2 and O2.
Overview of the Heating and Incubation System for Multiwell Plates – Blue Line
