ไมโครเพลทจำนวน 96 หลุมสีดำชนิด แบบ 3D

A µ-Plate with 96 wells for high-throughput 3D cell culture and tube formation assays

Applications

Specifications

Number of wells96
Length / width127.7 / 85.5 mm
Height with / without lid16.5 / 14.4 mm
Well-to-well distance9.0 mm
Volume inner wells10 µl
Ø Inner wells4 mm
Depth inner well0.8 mm
Volume upper wells70 µl
Ø Upper wells5 mm
Growth area per inner well0.125 cm²
Coating area per inner well0.23 cm²
Well clearance0.82 mm
Bottom: ibidi Polymer Coverslip



Technical drawings and details are available in the Instructions (PDF).

Find Out More

Please find more detailed information about the planning, conduction, and data analysis of angiogenesis assays here or download the “Angiogenesis” Application Guide as a PDF here.

Technical Features

*The gel matrix is not included with this product.

Every Cell in Focus:
The Principle of the µ-Plate 96 Well 3D

The µ-Plate 96 Well 3D has a specialized geometry for the easy, convenient, and reproducible conduction of tube formation assays. It is also ideal for sprouting assays, immunofluorescence staining, and general 3D cell culture.

After the gel has been pipetted into the inner well and given time to solidify, the cells can be seeded on top of it for tube formation analysis. Due to the “well-in-a-well” technology, the amount of gel needed is reduced to only 10 μl per well, which is 10% of the amount used in regular multiwell plates.

Further, no gel meniscus is formed. This ensures the formation of a uniformly thick gel matrix on which all cells are in one optical plane, creating reproducible cell culture conditions. The µ-Plate 96 Well 3D can be used with all common hydrogel matrices, such as Matrigel®, collagen gels, agarose gels, and hyaluronic acid gels.

With its 96 wells, the µ-Plate 96 Well 3D is designed for high throughput assays. For low throughput assays, ibidi provides the μ-Slide 15 Well 3D.

AN_plate_vs_standard.jpg

The µ-Plate 96 Well 3D Can Be Used in a Wide Range of Applications

Tube Formation Assay or Angiogenesis Assay

For a tube formation assay (also called angiogenesis assay), endothelial cells are seeded on top of a 10 µl gel layer (basement membrane-like surface), where they form capillary-like structures. Typically, Matrigel® is used. The tube formation ability of the cells can be used to analyze the pro- or anti-angiogenic potential of various drugs.

Find more detailed information here:

For practical details, please watch the ibidi movie Performing an Angiogenesis Assay (MV 15). In our Application Notes, you will find detailed information on the setup, optimization, data analysis, and interpretation of tube formation assays:

AN_tube_formation_assays.jpg



Phase contrast image showing one well of the µ-Slide 15 Well 3D with HUVEC cells on Matrigel® after 12 hours of incubation during a tube formation assay.


AN_assay_tube_formation_2.jpg
Phase contrast (top) and fluorescence microscopy (bottom) of a single strand composed of HUVEC cells during a tube formation assay in the µ-Slide 15 Well 3D. The F-actin cytoskeleton is stained green and the cell nuclei are stained blue.

Sprouting Assay

The sprouting potential of a cell cluster can be conveniently analyzed using the µ-Plate 96 Well 3D. To carry out a sprouting assay, spheroids or pieces of tissue (e.g., from the aorta), are placed onto or directly in the gel matrix before imaging.

AN_sprouting_assays.jpg

3D Cell Culture

In many cases, a 3D environment more closely resembles an in vivo situation than a 2D cell culture. The µ-Plate 96 Well 3D is an easy and cost-effective solution for the cultivation and microscopy of cells embedded in gel matrices. The gel layer is directly connected to the medium reservoir above, which allows for fast and easy medium exchange by diffusion.

Besides cultures with only one cell type, the invasion behavior of different cell types (e.g., cancer cells and fibroblasts) can be investigated in a co-culture experiment.

Alternatively, a sandwich culture can be created using the same or different 3D gel matrices (e.g., Matrigel or collagen).

Also, non-adherent cells, such as blood cells or bacteria, can be immobilized for enhanced microscopy access.

Immunofluorescence Staining

For analyzing the outcome of a tube formation or a 3D culture experiment, immunofluorescence staining can be performed in the µ-Plate 96 Well 3D. The cells can be fixated and stained directly on or in the gel, then imaged using high-resolution microscopy.

Focusing Cells

When using less than 10 µl of gel to fill the inner well of the µ-Plate 96 Well 3D, small numbers of cells can be cultivated and focused on a soft gel surface.

Low Volume Microscopy Chamber

The µ-Plate 96 Well 3D can also be used as a low-volume microscopy chamber without any gel, which is useful for experiments where several wells with a low volume are required.