คุณสมบัติเด่น

• เพลทใสชนิด 384 หลุม สำหรับการเพาะเลี้ยงเซลล์ (Cell Culture) และงานถ่ายภาพวิเคราะห์เซลล์ประสิทธิภาพสูง (High-Content Imaging)
• ก้นหลุมแบบ ibidi Polymer Coverslip #1.5 รองรับการถ่ายภาพความละเอียดสูงของเซลล์ทั้งแบบมีชีวิต (Living Cells) และเซลล์ที่ผ่านการตรึงสภาพ (Fixed Cells)
• ประกอบด้วย 384 หลุมทรงสี่เหลี่ยม ในมาตรฐาน ANSI/SLAS (SBS) Format รองรับระบบจ่ายสารอัตโนมัติ (Automated Liquid Handling) และงานคัดกรองตัวอย่างปริมาณสูง (High-Throughput Screening)
• ให้ความเรียบของพื้นผิวทั้งภายในแต่ละหลุมและทั่วทั้งแผ่นเพลทในระดับดีเยี่ยม ช่วยให้การโฟกัสภาพมีความสม่ำเสมอระหว่างการถ่ายภาพ

Cell Culture and Live Cell Imaging in a Clear 384 Well Plate

The µ-Plate 384 Well Clear is a clear 384 well plate for cell culture, high-content imaging, and automated cell-based assays. Transparent walls surround 384 square wells with a flat, optically clear #1.5 ibidi Polymer Coverslip bottom. Each well provides a growth area of 0.11 cm² and a recommended volume of 50 µl. The plate is available with ibiTreat or Untreated surfaces in an ANSI/SLAS format for manual and automated cell culture workflows.

Parallel Assays in 384 Square Wells

The 16 × 24 well array enables parallel comparison of treatments, controls, and biological replicates. The 4.5 mm well spacing and small culture volumes support assay miniaturization, while the coverslip bottom allows cells to be cultured and imaged directly in the same plate. The transparent plate body distinguishes this format from the black µ-Plate 384 Well.

Immunofluorescence and Protein Localization

The µ-Plate 384 Well Clear supports immunofluorescence staining and microscopy directly in the wells. Cell cultivation, fixation, staining, and imaging can be completed without transferring cells to separate coverslips. Parallel analysis enables comparison of staining conditions, protein localization, and cell morphology across experimental groups.

Immunofluorescence microscopy of rat fibroblasts (Rat1). Red: alpha-tubulin, green: F-actin, stained with LifeAct-TagGFP2 Protein; blue: nuclei (ibidi Mounting Medium with DAPI). A 60x oil immersion objective was used.

Live Cell Imaging

Live cell imaging enables comparison of reporter expression and cellular responses across transfection conditions, constructs, and treatments. The example below shows GFP-expressing Rat1 fibroblasts with a fluorescently labelled transfection reagent. A compatible ibidi Stage Top Incubator provides temperature, CO₂, and humidity control for long-term time-lapse experiments.

Live cell imaging of GFP expressing rat fibroblasts (Rat1) transfected with rhodamine labelled transfection reagent (METAFECTENE® FluoR, Biontex Laboratories).

Cell-Based Screening and Time-Lapse Studies

Image-based readouts enable assessment of cell morphology, proliferation, and responses to compounds across multiple conditions. The 384 well format supports parallel cell culture with small sample and reagent volumes. Time-lapse microscopy adds temporal information on cellular responses, complementing endpoint measurements in cell-based screening.

Microscopy Techniques

The #1.5 ibidi Polymer Coverslip supports direct brightfield, widefield fluorescence, and confocal microscopy on inverted systems. The Clear version has a polymer bottom only. The black µ-Plate 384 Well with #1.5H Glass Coverslip Bottom provides an alternative for glass-dependent TIRF or super-resolution workflows, subject to compatibility with the optical setup.

Specifications

For technical drawings and details, please see:
Instructions µ-Plate 384 Well Clear (PDF)

Technical Features

Precision, Quality, and Biocompatibility

• ANSI/SLAS (SBS) standard format
• Ready-to-use for lab automatization and high-throughput with robotics, fluorescence scanners, and plate readers
• 384 quadratic wells with standard numbering
• Excellent inner well flatness and whole plate flatness
• High-quality ibidi Polymer Coverslip bottom, for high-resolution microscopy
• No autofluorescence
• Compatible with staining and fixation solvents
• Compatible with immersion oil
• Non-cytotoxic and biocompatible
• Sterile with single packaging

Product Versions

• µ-Plate 384 Well Clear with #1.5 ibidi Polymer Coverslip and ibiTreat or Untreated surface
• Also available with black walls: µ-Plate 384 Well with #1.5 ibidi Polymer Coverslip or #1.5H Glass Coverslip Bottom

How Does the µ-Plate 384 Well Clear Work?

The µ-Plate 384 Well Clear combines 384 independent culture wells with a permanently integrated, thin #1.5 ibidi Polymer Coverslip bottom. Cells are cultured and imaged on the same surface, with optical access from below. The ibiTreat surface supports adherent cell culture, while the Untreated surface can be coated for cell models requiring a specific extracellular matrix. The wells and plate body are transparent.

µ-Plate 384 Well Clear at a Glance

The ibidi µ-Plate Range

	6 Well	12 Well	24 Well	96 Well Round	96 Well Square	384 Well	384 Well Clear
Bottom
Volume / well	5 ml	3 ml	1 ml	200 µl	300 µl	50 µl	50 µl
Growth area / well	9.1 cm²	3.6 cm²	1.54 cm²	0.3 cm²	0.56 cm²	0.11 cm²	0.11 cm²

The ibidi µ-Plates are specifically designed for cell culture, immunofluorescence, and high-end microscopy. They offer flexibility in well size, well volume, and bottom materials. The range includes formats from 6 to 384 wells. All ibidi multiwell plates are user-friendly and ideal for multiplexing and high-throughput applications, providing high optical quality and compatibility with robotic systems. Most µ-Plates are available with either a #1.5 ibidi Polymer Coverslip or #1.5H Glass Coverslip bottom.

Packaging Information