จานเพาะเลี้ยงเซลล์ สำหรับการถ่ายภาพ ขนาด 35 มม. ชนิดขอบต่ำ ก้นทำจากแก้ว

A 35 mm imaging dish with a glass bottom and low walls for TIRF, single molecule and super-resolution microscopy

Applications

Want to know if you should use a glass or a polymer bottom for your application? Find out here.

Specifications

Ø µ-Dish35 mm
Volume800 µl
Growth area3.5 cm2
Coating area using 400 µl4.1 cm2
Ø observation area21 mm
Height with / without lid9 / 7 mm
Bottom: Glass Coverslip No. 1.5H, selected quality, 170 µm +/- 5 µm

Fluorescence microscopy of fixed rat fibroblasts on a Glass Coverslip #1.5H. F-actin filaments are stained with phalloidin (green), nuclei are stained with DAPI (blue).

Technical Features

The Principle of the µ-Dish 35 mm, low Glass Bottom

Low Walls for Easy Micromanipulation of Cells

The ibidi μ-Dishes with low walls (i.e., μ-Dish 35 mm, low and µ-Dish 35 mm, low Glass Bottom) are perfectly suited for micromanipulation assays, such as intracellular injections, micropipette chemotaxis assays, or tissue treatment and microscopy. Due to the low walls of these µ-Dishes, instrumentation (e.g., electrodes) can be positioned exactly using a micromanipulator. The excellent optical quality makes the ibidi µ-Dishes ideally suited for single-cell handling and high-resolution live cell imaging. For all standard cell culture applications, we also offer the µ-Dish 35 mm, high product family with high walls.

The Coverslip Bottom

The µ-Dish 35 mm, low Glass Bottom comes with a thin #1.5H Glass Coverslip Bottom made from D 263 M Schott borosilicate glass that has the highest optical quality. ibidi developed these glass surfaces specifically for TIRFsuper-resolution microscopy, and single molecule microscopy. The dish is also available with an #1.5 ibidi Polymer Coverslip Bottom for optimal cell adhesion and high-resolution microscopy in a variety of assays.

Learn more about the coverslip bottom or the different surfaces of the ibidi chambers here.

Lid with Locking Feature for Minimized Evaporation

All ibidi µ-Dishes are equipped with the special lid-locking feature. The locking position minimizes evaporation, and thereby provides excellent conditions for long-term studies in a non-humidified environment. Gas exchange (carbon dioxide or oxygen) during cell culture is maintained thanks to the gas-permeable plastic material of the dish.

TIP: Use the locking feature if minimal evaporation is required (e.g., outside incubators, non-humidified microscopy stages, etc.).

Experimental Example

Microinjection of cells using the µ-Dish 35 mm, low Glass Bottom. The microcapillary (e.g., Eppendorf Femtotips®) is connected to a micromanipulator, which is placed directly on the stage of the confocal fluorescence microscope. The low walls of the µ-Dish allow the capillary to easily access the cells.

Comparison of the ibidi Dishes 35 mm, low

Please find a detailed comparison of the material specifications, including suitable microscopy applications for the ibidi Polymer Coverslip, the ibidi Glass Bottom, and further materials, here.

µ-Dish 35 mm, lowµ-Dish 35 mm, lowµ-Dish 35 mm, low Glass Bottomµ-Dish 35 mm, low Glass Bottom
Bottom material#1.5 ibidi Polymer Coverslip#1.5H ibidi Glass Coverslip
Bottom thickness180 µm (+10/-5 µm)170 µm (+/-5 µm)
Bottom: gas permeabilityYesNo
Available surfacesibiTreat (tissue culture-treated),
Uncoated (hydrophobic)
Uncoated glass
LidLid with locking featureLid with locking feature
PackagingSterile, individually packedSterile, individually packed
Quantity60 pcs./box60 pcs./box
High wall version available?Yes: µ-Dish 35 mm, high for all standard cell culture applicationsYes: µ-Dish 35 mm, high Glass Bottom (#1.5H) with highest optical quality and the cost-effective Glass Bottom Dish 35 mm (#1.5)
Gridded version available?Yes: µ-Dish 35 mm, low Grid-500No

The ibidi µ-Dishes: High Walls vs. Low Walls

µ-Dish 35 mm, high with high walls for all standard cell culture applications

µ-Dish 35 mm, low with low walls provides a larger access area useful for micromanipulation