Chamber slide แบบซิลิโคน 3 ช่อง สามารถดึงออกได้ – สำหรับการเพาะเลี้ยงเซลล์ และการย้อม immunofluorescence 

Chamber slide for time-saving cell culture, immunofluorescence staining, and imaging—with a removable 3 well chamber

Applications

Specifications

Number of wells3
Dimensions of wells (w x l x h) in mm16.5 x 16.5 x 8.0
Volume per well1100 µl (150 µl*)
Growth area per well1.66 cm²
Coating area per well3.73 cm²
Gasket materialBiocompatible silicone
Suitable coverslip size24 mm x 60 mm
BottomGlass slide (26 x 76 x 1 mm)

* Volume reduction during staining when using Ø 15 mm Coverslips for 3 Well Chamber, removable

Technical Features

The Principle of the Chamber Slides, removable

In the ibidi Chamber Slides, a silicone gasket with separated chambers is mounted on a standard 1 mm glass slide. The ibidi Chamber Slides are ideal for immunofluorescence stainings: Once mounted with a glass coverslip, you can perform upright and inverted high-resolution microscopy. Afterwards, you can store the mounted samples for a long time.

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The easily removable silicone gasket offers several possibilities during immunofluorescence stainings: For high-throughput screenings, the silicone gasket can be removed before start­ing the staining. This allows for the processing of several slides at once in a staining jar. As an alternative (in low-throughput experiments), the silicone gasket can be left in position, allowing for separate stainings in each well.

A Chamber Slide for Cell Culture, Staining, and Imaging

Experimental Example

Immunofluorescence staining of primary mouse brain microvascular endothelial cells (pMBMECs), cultured in the ibidi 12 Well Chamber, removable. Red color delineates the endothelial cell junctions labeled for zonula occludens (ZO)-1, highlighting their matured state. Blue color shows the nuclei stained with DAPI. The image was acquired with a 20x objective on a Nikon Eclipse microscope.

Data by Sidar Aydin, Britta Engelhardt, Ruth Lyck, Theodor Kocher Institute, Bern, Switzerland.