ibidi : µ-Slide VI 0.5 Glass Bottom

ibidi - 80607

µ-Slide VI 0.5 Glass Bottom



µ-Slide แบบ 6 ช่องสำหรับทำงาน flow assays และสำหรับ immonofluorescence

  • สำหรับงาน immunofluorescence
  • เซลล์กระจายตัวในช่องว่างอย่างทั่วถึง
  • ประหยัดค่าใช้จ่ายในการทำการทดลองเนื่องมาจากใช้เซลล์จำนวนน้อย และใช้ reagent ในปริมาณน้อย
  • สามารถใช้ในการถ่ายรูปได้ดีเนื่องมาจากก้นภาชนะทำมาจากแก้วที่มีความบางเท่ากับ coverslip (# 1.5H)
  • มี 15 ชิ้น/ 1 กล่อง

A 6 channel μ-Slide suitable for flow experiments and for immunofluorescence assays

  • All-in-one chamber that simplifies immunofluorescence protocols
  • Homogeneous cell distribution over the channel surface, regardless of handling practices
  • Cost-effective experiments with small numbers of cells and low volumes of reagents
  • Perfect cell imaging thanks to the low thickness variability of the coverslips’ glass (# 1.5H)
  • Surface Modification: #1.5H (170 µm +/- 5 µm) D 263 M Schott glass, sterilized
    Pcs./Box: 15 (individually packed)



Outer dimensions (w x l)75.5 x 25.5 mm²
AdaptersFemale Luer
Number of channels6
Channel volume40 µl
Channel height0.54 mm
Channel length17 mm
Channel width3.8 mm
Volume per reservoir60 µl
Height with/without lid8.8 mm/7.6 mm
Growth area0.6 cm² per channel
Coating area using 40 µl1.2 cm² per channel
Bottom: Glass coverslip No. 1.5H, selected quality, 170 µm +/- 5 µm

Technical Features

  • 40 µl channel volume, which saves on reagent consumption
  • No. 1.5H High Precision D 236M borosilicate glass coverslip
  • Fully compatible with high resolution fluorescence microscopy
  • Easy connection to existing tubes and pumps via female Luer adapter
  • Defined shear stress and shear rate level
  • Also available as a µ-Slide VI 0.4 with a Polymer Coverslip Bottom

All-in-One-Chamber for Fast Immunofluorescence Protocols

Application Example: PAINT Super-Resolution Microscopy of B Cells

Super-resolution PAINT microscopy of fixed B cells in the µ-Slide VI 0.5 Glass Bottom, using a TIRF microscope. LifeAct-Cy3B was used to visualize the F-actin cytoskeleton. Objective lens: Nikon CFI SR HP Apochromat TIRF 100x Oil immersion, NA=1.49. Courtesy of Massive Photonics GmbH (www.massive-photonics.com).

ibidi Polymer Coverslip vs. ibidi Glass Bottom

ibidi Polymer Coverslipibidi Glass Bottom
Optical properties
Refractive index (nD 589 nm)1.521.52
Abbe number5655
Thickness#1.5 (180 µm)#1.5H (170 µm)
MaterialMicroscopy plasticD 263M Schott borosilicate glass
TransmissionVery high (even ultraviolet)High (ultraviolet restrictions)
Birefringence (DIC)Low (DIC compatible*)Low (DIC compatible*)
Other aspects
Surface modificationsibiTreat – tissue culture treated
Uncoated – hydrophobic
Only glass
Protein coatingsCompatibleCompatible
Gas permeableYesNo
Material flexibilityHighLow
ApplicationsFluorescence microscopyTIRF and single photon