ibidi : µ-Dish 35 mm, high Grid-50 Glass Bottom

ibidi - 81148

µ-Dish 35 mm, high Grid-50 Glass Bottom 

จานเพาะเลี้ยงเซลล์แบบก้นแก้ว ขนาด 35 มม แบบมี grid 50 ไมครอน ชนิดขอบสูง



จานเพาะเลี้ยงเซลล์แบบก้นแก้ว สำหรับการถ่ายภาพ ขนาด 35 มม. แบบมี grid ขนาด 50 ไมครอน เพื่อการหาตำแหน่งเซลล์

  • ออกแบบมาสำหรับการหาตำแหน่งเซลล์
  • ง่ายต่อการนับเซลล์ต่อพื้นที่
  • ก้นภาชนะทำมาจากแก้วเหมาะสำหรับกล้องจุลทรรศน์แบบ TIRF

A 35 mm imaging dish with a glass bottom and an imprinted 50 µm cell location grid

  • Ideal for locating cells or cell clusters
  • Easy cell counting per defined area
  • Glass coverslip for TIRF and single molecule applications
  • Surface Modification: #1.5H (170 µm +/- 5 µm) D 263 M Schott glass, sterilized
    Pcs./Box: 30 (individually packed)
    Grid: Grid-50


  • Locating cells or cell clusters, e.g., transfected cells for clone picking
  • Counting events per defined area (e.g., when calculating transfection efficiency)
  • Providing a reference structure for cell movements
  • Treatment of distinct single cells, such as in microinjection

Want to know if you should use a glass or a polymer bottom for your application? Find out here.


Geometry of the µ–Dish 35 mm, high Glass Bottom

Diameter dish35 mm
Volume2 ml
Growth area3.5 cm2
Coating area using 400 µl4.1 cm2
Diameter observation area21 mm
Height with / without lid14 mm / 12 mm
Bottom: Glass coverslip No. 1.5H, selected quality, 170 µm +/- 5 µm

Geometry of the Grid-50

Number of squares4 x 400
Repeat distance50 µm (+/- 1 %)
Groove width5 µm (± 1 µm)
Groove depth< 5 µm

Technical Features

  • Grid with 50 µm µm repeat distance
  • Lettered and numbered 4 x 400 squares (A-U; 1-20)
  • Grid and cells in one focal plane
  • Bottom made from D 263 M Schott glass with a thickness of 170 µm +/- 5 µm
  • Lid with lock position, which minimizes evaporation
  • May require coating to promote cell attachment
  • Also available in a Grid-500 version with ibidi Polymer Coverslip Dish 35 mm, high Grid-500 or µ-Slide 8 Well Grid-500

Layout Grid-50

ibidi Polymer Coverslip vs. ibidi Glass Bottom

ibidi Polymer Coverslipibidi Glass Bottom
Optical properties
Refractive index (nD 589 nm)1.521.52
Abbe number5655
Thickness#1.5 (180 µm)#1.5H (170 µm)
MaterialMicroscopy plasticD 263M Schott borosilicate glass
TransmissionVery high (even ultraviolet)High (ultraviolet restrictions)
Birefringence (DIC)Low (DIC compatible*)Low (DIC compatible*)
Other aspects
Surface modificationsibiTreat – tissue culture treated
Uncoated – hydrophobic
Only glass
Protein coatingsCompatibleCompatible
Gas permeableYesNo
Material flexibilityHighLow
ApplicationsFluorescence microscopyTIRF and single photon

* Special glass DIC Lids are available separately