จานเพาะเลี้ยงเซลล์แบบก้นแก้ว สำหรับการถ่ายภาพ ขนาด 35 มม. แบบมี grid ขนาด 50 ไมครอน เพื่อการหาตำแหน่งเซลล์

A 35 mm imaging dish with a glass bottom and an imprinted 50 µm cell location grid

Applications

Want to know if you should use a glass or a polymer bottom for your application? Find out here.

Specifications

Geometry of the µ–Dish 35 mm, high Glass Bottom

Diameter dish35 mm
Volume2 ml
Growth area3.5 cm2
Coating area using 400 µl4.1 cm2
Diameter observation area21 mm
Height with / without lid14 mm / 12 mm
Bottom: Glass coverslip No. 1.5H, selected quality, 170 µm +/- 5 µm


Geometry of the Grid-50

Number of squares4 x 400
Repeat distance50 µm (+/- 1 %)
Groove width5 µm (± 1 µm)
Groove depth< 5 µm

Technical Features

Layout Grid-50

ibidi Polymer Coverslip vs. ibidi Glass Bottom

ibidi Polymer Coverslipibidi Glass Bottom
Optical properties
Refractive index (nD 589 nm)1.521.52
Abbe number5655
Thickness#1.5 (180 µm)#1.5H (170 µm)
MaterialMicroscopy plasticD 263M Schott borosilicate glass
AutofluorescenceLowLow
TransmissionVery high (even ultraviolet)High (ultraviolet restrictions)
Birefringence (DIC)Low (DIC compatible*)Low (DIC compatible*)
Other aspects
Surface modificationsibiTreat – tissue culture treated
Uncoated – hydrophobic
Only glass
Protein coatingsCompatibleCompatible
Gas permeableYesNo
Material flexibilityHighLow
BreakableNoYes
ApplicationsFluorescence microscopyTIRF and single photon

* Special glass DIC Lids are available separately