คุณสมบัติเด่น
- ไมโครฟลูอิดิกแชนแนลสไลด์ (Microfluidic Channel Slide) สำหรับการสร้างแบบจำลองเนื้อเยื่อ (Tissue Models) และงานวิจัยด้าน Organ-on-a-Chip
- เหมาะสำหรับการสร้างสภาพแวดล้อมเซลล์แบบสามมิติ (3D Microenvironment) รวมถึงการสร้างโครงสร้างและลวดลาย 2D/3D ด้วยเทคนิคการกระตุ้นด้วยแสง (Light-Induced Microfabrication and Patterning)
- ออกแบบเป็นไมโครฟลูอิดิกแชนแนลพร้อมพอร์ตเชื่อมต่อมาตรฐานแบบ Luer ช่วยให้สามารถเชื่อมต่อกับระบบปั๊ม (Pump) ได้อย่างสะดวกและต่อเนื่อง
- มาพร้อมก้นสไลด์แบบ #1.5 Polymer Coverslip Bottom คุณภาพสูง รองรับการถ่ายภาพและวิเคราะห์ตัวอย่างขั้นสูงด้วยกล้องจุลทรรศน์ความละเอียดสูง
Applications
The µ-Slide Tissue Engineering provides a flexible platform for designing and studying complex 2D and 3D tissue models under controlled fluidic conditions.
Tissue and Organ-on-a-Chip Models
- Custom 3D structures: Fabricate microchannels, bifurcations, or flow barriers by photoinduced polymerization using photocrosslinkable hydrogels such as GelMA, PEG-DA, collagen, or hyaluronic acid.
- Tissue and organ models: Build microscale models of tissues (e.g., vascular networks or tumor microenvironments) for studying cell behavior, drug effects, or tissue regeneration.
- Generation of topographic surfaces: Mimic tissue microenvironments by replicating the structure and stiffness of native tissue.
Microfluidics
- High-precision microfluidics: Seamlessly integrate the ibidi Pump System to control perfusion of microchannels and hydrogel structures.
- Modeling interstitial flow: Mimic how fluids move through tissues to study how cells sense and respond to shear stress.
- Gradient formation: Generate gradients in 3D by dynamic perfusion.
- Dynamic microenvironments: Use controlled perfusion to maintain cell viability and mimic changing physiological conditions.
Microscopy Techniques
- Brightfield, phase contrast, and widefield fluorescence microscopy for cell monitoring on inverted microscopes
- Live cell imaging and time-lapse studies over extended time periods
- Confocal microscopy, two-photon microscopy, FRAP, FRET, FLIM, and LSFM
Philipp’s Trick:
What’s an Easy Way to Seal and Perfuse 3D Bioprinted Structures?
Find out on our Tips and Tricks page!
Specifications
| Outer dimensions (w × l) | 25.5 × 75.5 mm |
| Adapters/reservoirs | Female Luer |
| Volume workspace | 30 µl |
| Height workspace | 0.4 mm |
| Growth area workspace | 0.77 cm2 |
| Coating area workspace | 1.56 cm2 |
| Volume side channels | 115 µl each |
| Height side channels | 1.6 mm |
| Growth area side channels | 0.64 cm2 each |
| Coating area side channels | 2.00 cm2 each |
| Volume per reservoir | 60 µl |
| Bottom material | #1.5 ibidi Polymer Coverslip (different surfaces available) |
For technical drawings and details, please see:
Instructions µ-Slide Tissue Engineering (PDF)
Technical Features
Precision, Quality, and Biocompatibility
- Microfluidic channel slide with 2 workspaces, each flanked by parallel channels
- #1.5 ibidi Polymer Coverslip with excellent optical quality
- Compatible with fluidic photocrosslinkable hydrogels or extracellular matrices
- Compatible with staining and fixation solutions
- Compatible with immersion oil
- Made from non-cytotoxic, biocompatible materials
- Sterile with single packaging
- Fully compatible with high-resolution fluorescence microscopy
- Ideal in combination with the ibidi Micro Illumination System
- Standard Luer ports: compatible with multiple flow and perfusion setups (e.g., ibidi Pump System)
Product Versions
- Also available: the sticky-Slide Tissue Engineering, offering unlimited access to the workspace (e.g., for additive 3D printing)
How Does the µ-Slide Tissue Engineering Work?
What Is the Principle of the µ-Slide Tissue Engineering?
The µ-Slide Tissue Engineering provides a flexible platform for designing and studying complex 2D and 3D tissue models under controlled fluidic conditions. The slide consists of a central workspace flanked by parallel channels. The workspace can be filled and structured with photocrosslinkable hydrogels such as GelMA, PEG-DA, collagen, or hyaluronic acid.
These hydrogels can be polymerized into defined patterns using the ibidi Micro Illumination System or similar systems. The design of the slide allows for seamless integration with the ibidi Pump System for controlled perfusion, simulating physiological flow and nutrient transport.
What Is the µ-Slide Tissue Engineering Used For?
The µ-Slide Tissue Engineering is designed for advanced 2D and 3D cell culture experiments that require a realistic tissue environment and fluid control, such as tissue and organ modeling, flow and perfusion studies, and gradient formation.
Can I Use Photopatterning or Microstructuring With the µ-Slide Tissue Engineering?
Yes. With the ibidi Micro Illumination System, for example, you can photopattern or microstructure to define where cells adhere, grow, and migrate. This technique allows for the creation of highly controlled tissue architectures, including aligned neurons, patterned co-cultures, and wound-healing models. Additionally, to mimic native tissue environments and guide cell behavior, surface topographies and protein patterns can be generated.
Structural Examples
The central workspace and side channels can be structured using photocrosslinkable hydrogels, defining the locations for the cell/tissue model and fluidic access.
Is the µ-Slide Tissue Engineering Compatible With the ibidi Pump System?
Yes. The µ-Slide Tissue Engineering can be easily connected to pump systems such as the ibidi Pump System via the Luer adapters for precise control of perfusion rates and flow profiles. This setup allows you to simulate physiological conditions such as blood flow, interstitial flow, or dynamic shear stress. The channels can be perfused in parallel to achieve complex flow scenarios.
Flow Setup Examples
Depending on the structure, various perfusion methods can be used, ranging from perfusing a single channel to multiple flows in parallel.
Is the µ-Slide Tissue Engineering Suitable for Live Cell Imaging?
Yes. The slide features a high-optical-quality bottom for excellent imaging with widefield, confocal, or live cell microscopy, and it maintains physiological conditions when used with ibidi Stage Top Incubators or other incubators.
Do I Need the ibidi Micro Illumination System to Use the µ-Slide Tissue Engineering?
You can fill the hydrogel or scaffold without any structuring, or you can perform 2D or 3D photostructuring with the ibidi Micro Illumination System or other photoinduced technologies.
Application Examples
Stitched phase contrast image of 200 µm PEG-NB hydrogel pillars, created in the ibidi µ-Slide Tissue Engineering.
Packaging Information
| Order No. | Packaging Unit | Packaging Dimensions (w × l × h) | Shipping Weight |
| 80231, 80236 | 15 (indiv. packed) | 150 × 130 × 110 mm | 0.2 kg |
| 80231-90, 80236-90 | 90 (indiv. packed) | 295 × 225 × 175 mm | 1.4 kg |
| 80231-120, 80236-120 | 120 (8 per tray) | 295 × 225 × 133 mm | 1.9 kg |
