ibidi : µ-Slide I Luer Glass Bottom

Channel slides with a #1.5H glass bottom for flow applications; different heights and volumes available

Large area of uniform shear stress
Easy connection using Luer adapters
Homogeneous cell distribution with defined optical pathway
Super-resolution and TIRF possible due to the #1.5H glass substrate
Surface Modification: #1.5H (170 µm +/- 5 µm) D 263 M Schott glass, sterilized
Pcs./Box: 15 (individually packed)
Channel Version (Channel Height): 0.2 (Channel Height 250 µm)
Surface Modification: #1.5H (170 µm +/- 5 µm) D 263 M Schott glass, sterilized
Pcs./Box: 15 (individually packed)
Channel Version (Channel Height): 0.4 (Channel Height 450 µm)
Surface Modification: #1.5H (170 µm +/- 5 µm) D 263 M Schott glass, sterilized
Pcs./Box: 15 (individually packed)
Channel Version (Channel Height): 0.6 (Channel Height 650 µm)
Surface Modification: #1.5H (170 µm +/- 5 µm) D 263 M Schott glass, sterilized
Pcs./Box: 15 (individually packed)
Channel Version (Channel Height): 0.8 (Channel Height 850 µm)

Applications

Adherent cells under flow conditions
Cell culture (static or stop-flow)
Simulation of blood vessels with endothelial cells
High-resolution microscopy of living and fixed cells
TIRF and super-resolution microscopy

Want to know if you should use a glass or a polymer bottom for your application? Find out here.

Specifications

Outer dimensions	25.5 x 75.5 mm² (w x l)
Channel length	50 mm
Channel width	5 mm
Adapters	Female Luer
Volume per reservoir	60 μl
Growth area	2.5 cm²
Bottom: Glass coverslip No. 1.5H, selected quality, 170 µm +/- 5 µm

Because of the adhesive layer, the channel height of the µ-Slide I Luer Glass Bottom increases by 50 µm compared to the µ-Slide I Luer.

µ-Slide I Luer Glass Bottom	0.2	0.4	0.6	0.8
Channel height	250 µm	450 µm	650 µm	850 µm
Channel volume	62.5 µl	112.5 µl	162.5 µl	212.5 µl
Coating area	5.2 cm²	5.4 cm²	5.6 cm²	5.8 cm²

Find Out More

Please find more detailed information about the planning, conduction, and data analysis of cell culture under flow assays here.

Download the whole “Cell Culture Under Flow” Application Guide as a PDF here.

Technical Features

Standard format with thin coverslip bottom made from D 263 M Schott glass #1.5H (170 µm +/- 5 µm) for low or high magnification microscopy
Large observation area for microscopy
Defined shear stress and shear rate levels
Easy connection to tubes and pumps using Luer adapters
Fully compatible with the ibidi Pump System
May require coating to promote cell attachment
Also available as a µ-Slide I Luer with an ibidi Polymer Coverslip Bottom for superior cell growth

The Principle of the µ-Slide I Luer Glass Bottom

The Glass Bottom

The µ-Slide I Luer Glass Bottom comes with a thin #1.5H Glass Coverslip Bottom that has the highest optical quality and is ideally suitable for high-resolution microscopy, TIRF, and super-resolution microscopy. To promote cell attachment, a surface coating might be required prior to cell seeding.

Find more information and technical details about the coverslip bottom of the ibidi chambers here.

Choosing the Right Channel Height for Flow Applications

Which Experiment Are You Planning?

Low channels are more suitable for flow applications.
High channels are more suitable for static cell culture.

For flow assays with small amounts of medium and high values of shear stress	0.2 channel
For a wide range of shear stress	0.4 channel
For controlling low values of shear stress < 2 dyne/cm²	0.6 and 0.8 channels

Select the ideal Perfusion Set for your flow application here.