A chambered coverslip with 8 individual wells, high walls, and a #1.5H glass coverslip bottom, suitable for use in TIRF and single molecule applications
- A cell culture chamber for observation of the sample through a glass coverslip bottom using high resolution microscopy
- Brilliant cell imaging thanks to the low thickness variability of the coverslips’ glass
- Cost-effective experiments using small numbers of cells and low volumes of reagents
- Extra high individual walls to keep cross contamination between wells as low as possible
- A chambered coverslip with 8 individual wells, high walls, and a #1.5H glass coverslip bottom, suitable for use in TIRF and single molecule applications
- Surface Modification: #1.5H (170 µm +/- 5 µm) D 263 M Schott glass, sterilized
Pcs./Box: 15 (individually packed) - Surface Modification: #1.5H (170 µm +/- 5 µm) D 263 M Schott glass, sterilized
Pcs./Box: 90 (individually packed) - Surface Modification: #1.5H (170 µm +/- 5 µm) D 263 M Schott glass, sterilized
Pcs./Box: 96 (12 trays with 8 pieces each)
Applications
- Cultivation and high-resolution microscopy of cells
- TIRF and single molecule applications of living and fixed cells
- Super-resolution microscopy
- Immunofluorescence staining and fluorescence microscopy of living and fixed cells
- Live cell imaging over extended time periods
- Transfection assays
- Differential interference contrast (DIC) microscopy when used with a DIC lid
Want to know if you should use a glass or a polymer bottom for your application? Find out here.
Specifications
| Outer dimensions (w x l) | 25.5 x 75.5 mm² |
| Number of wells | 8 |
| Dimensions of wells (w x l x h) | 9.4 x 10.7 x 9.3 mm³ |
| Volume per well | 300 µl |
| Total height with/without lid | 10.8/9.5 mm |
| Growth area per well | 1.0 cm² |
| Coating area per well | 2.2 cm² |
| Bottom: Glass coverslip No. 1.5H, selected quality, 170 µm +/- 5 µm | |
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| 80807 Standard Pack Individually packed | 80807-90 Bulk Pack Individually packed | 80807-96 Bulk Pack 8 Slides per tray |
Technical Drawing
Technical drawings and details are available in the Instructions (PDF).
Technical Features
- Chambered coverslip with 8 independent wells and a non-removable glass coverslip-bottom
- Bottom made from D 263 M Schott glass, No. 1.5H
(170 +/- 5 µm) - May require coating to promote cell attachment
- Individual well walls for minimizing well-to-well crosstalk and contaminations
- Compatible with staining and fixation solutions
- Available as a Bulk Pack with 90 individually packed µ-Slides per box
- Also available as a µ-Slide 8 Well high with an ibidi Polymer Coverslip Bottom for superior cell growth
- Also available as an adhesive version without a bottom:
sticky-Slide 8 Well high - Additional version available with a 500 µm grid:
µ-Slide 8 Well high Grid-500 - Available with a non-adhesive Bioinert surface: µ-Slide 8 Well high Bioinert, specifically suitable for 3D applications, such as spheroids and suspension cells
- Available with a µ-Patterned surface with defined cell adhesion for single-cell and multi-cell applications
The Principle of the µ-Slide 8 Well high
The Coverslip Bottom
The µ-Slide 8 Well high Glass Bottom comes with a high precision D263 M Schott glass, No 1.5H (170 +/– 5 µm) Glass Coverslip bottom. This bottom is ideally suitable for high-resolution microscopy and special microscopic applications like TIRF or super-resolution.
Find more information about the different surfaces of the ibidi chambers here.
Application Examples
Immunofluorescence
The ibidi µ-Slide 8 Well high Glass Bottom allows for standard immunofluorescence protocols to be employed without the use of coverslips in an all-in-one chamber. All steps (e.g., cell cultivation, fixation, staining, and imaging) are carried out in the open well geometry. After staining, the sample can be observed through the coverslip bottom using high-resolution microscopy.
Fluorescence staining of adherent fibroblast cells.
Blue: nuclei (DAPI). Green: F-actin cytoskeleton (Alexa488-Phalloidin), widefield fluorescence, objective lens 40x, oil immersion.
Live Cell Imaging
The µ-Slide 8 Well high Glass Bottom enables high-resolution live cell imaging using different brightfield and fluorescence techniques including TIRF and super-resolution microscopy.
Together with the ibidi Stage Top Incubation System, you can keep your cells happy for a long time by precisely controlling temperature, humidity, and CO2 concentration on your microscope.
Live Cell Imaging of rat fibroblasts in the µ-Slide 8 Well high Glass Bottom.
The ibidi Stage Top Incubation System was used to keep the cells at 37°C and supply 5% CO2. Phase contrast and widefield fluorescence imaging at 3 min intervals, 60x objective lens, oil immersion. Nuclei in blue (NucBlue™ Live ReadyProbes™ Hoechst 33342).
