Chamber slide แบบซิลิโคน 12 ช่อง สามารถดึงออกได้ – สำหรับการเพาะเลี้ยงเซลล์ และการย้อม immunofluorescence
- ประหยัดค่าใช้จ่าย โดยเหมาะสำหรับงาน immunofluorescence และการเก็บตัวอย่างเป็นเวลานาน
- สามารถใช้กับกล้องจุลทรรศน์ทั้งแบบ upright และ inverted
- สามารถใช้เซลล์ อาหารเลี้ยงเซลล์ได้ในปริมาณน้อย
Chamber slide with a removable 12 well chamber for time-saving cell culture, immunofluorescence staining, and imaging
- For cell cultivation, fixation, staining, and imaging on one single microscopy slide—no cell transfer needed
- Ideal for upright or inverted microscopes, and long-term storage of samples
- Removable silicone chamber with 12 wells on a standard microscope glass slide
- Pcs./Box: 15 (individually packed) Surface Modification: microscopy glass slide, sterilized
Applications
- Time-saving and cost-effective immunofluorescence assays with subsequent long-term sample storage
- Fluorescence‐based immunocytochemistry (ICC) assays
- High-resolution imaging (e.g., widefield fluorescence or confocal microscopy)
- Upright and inverted microscopy
Specifications
| Number of wells | 12 |
| Dimensions of wells (w x l x h) in mm | 7.5 x 7.5 x 8 |
| Volume per well | 250 µl |
| Growth area per well | 0.56 cm² |
| Coating area per well | 1.90 cm² |
| Gasket material | Biocompatible silicone |
| Suitable coverslip size | 24 mm x 60 mm |
| Bottom | Glass slide (26 x 76 x 1 mm |
Technical Features
- Self-adhesive silicone chamber with 3 wells mounted on a standard 1 mm glass slide
- Cells grow on a standard 1 mm glass slide (may require coating to promote cell attachment)
- Non-fluorescent glass slide with frosted ends for easy handling and labeling
- Gasket made out of fully biocompatible silicone material
- No cell transfer needed prior to staining and imaging
- Easy removal of the chambered silicone gasket (e.g., for high-throughput stainings)
- Suitable for use with upright and inverted microscopes after mounting with Coverslips for Chamber Slides, removable
- Compatible with all standard mounting media (e.g., ibidi Mounting Medium or ibidi Mounting Medium With DAPI)
- Optional: the chambered gasket may be transferred to any flat, clean surface for cell cultivation
- Available with 3 wells, 8 wells, and 12 wells
The Principle of the Chamber Slides, removable
In the ibidi Chamber Slides, a silicone gasket with separated chambers is mounted on a standard 1 mm glass slide. The ibidi Chamber Slides are ideal for immunofluorescence stainings: Once mounted with a glass coverslip, you can perform upright and inverted high-resolution microscopy. Afterwards, you can store the mounted samples for a long time.
The easily removable silicone gasket offers several possibilities during immunofluorescence stainings: For high-throughput screenings, the silicone gasket can be removed before starting the staining. This allows for the processing of several slides at once in a staining jar. As an alternative (in low-throughput experiments), the silicone gasket can be left in position, allowing for separate stainings in each well.
A Chamber Slide for Cell Culture, Staining, and Imaging
Experimental Example
Immunofluorescence staining of primary mouse brain microvascular endothelial cells (pMBMECs), cultured in the ibidi 12 Well Chamber, removable. Red color delineates the endothelial cell junctions labeled for zonula occludens (ZO)-1, highlighting their matured state. Blue color shows the nuclei stained with DAPI. The image was acquired with a 20x objective on a Nikon Eclipse microscope.
Data by Sidar Aydin, Britta Engelhardt, Ruth Lyck, Theodor Kocher Institute, Bern, Switzerland.
