จานเพาะเลี้ยงเซลล์พร้อมซิลิโคนแบบ 4 ช่อง – สำหรับการติดตามแต่ละเซลล์ ทั้งเซลล์เกาะติดและเซลล์แขวนลอย
- สำหรับการใช้กล้องจุลทรรศน์ในระยะยาว
- การเพาะเลี้ยงเซลล์จำนวนที่น้อยโดยปราศจากการระเหย
- ใน well มีลักษณะคล้ายรูปทรงกรวยเพื่อการมองภาพที่ยอดเยี่ยม
- สามารถใช้เลนส์วัตถุกำลังขยาย 20 เท่าเพื่อดูพื้นที่ทั้งหมด
- มีเทคโนโลยี ibiTreat surface เพื่อการเจริญเติบโตของเซลล์ในอุดมคติ
A 4 well silicone insert used for tracing individual cells (adherent and suspension)
- Allows for long-term microscopy assays of single cells
- Cultivation of small numbers of cells without evaporation risks
- Conical wells for superb optical quality
- Complete coverage of the well using a 20x objective
- Individual inserts for single experiments
- Ideal cell growth on ibiTreat surface
- Surface Modification: ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized
Pcs./Box: 30 (individually packed)
Product Variation: in µ-Dish 35 mm, high
Applications
- Live cell imaging of adherent and suspension cells
- Immunofluorescence assays
- Performing long-term microscopy assays lasting up to several weeks
- Cell tracking of single cells over time
- Cell differentiation (e.g., single stem cells)
Specifications
| Number of wells | 4 |
| Dimensions of wells in mm | Ø 0.4 |
| Diameter of the complete insert | 12 mm |
| Height of the complete insert | 4.3 mm |
| Volume per well | 10 µl |
| Growth area per well | 0.0012 cm2 |
| Coating area per well | 0.188 cm2 |
| Material | Biocompatible silicone |
| Bottom | No bottom – sticky underside |
Technical Features
- Four small Ø 0.4 mm microscopy wells
- Suspension cells cannot escape the observation field
- Adhesive underside
- Transferable to any flat, clean surface
- No material is left behind after the insert is removed
- Includes a rim for easy handling of the insert with tweezers
Experimental Example
Cultivation of IVF mouse eggs in the micro-Insert 4 Well FulTrac (prototype; total imaging duration: 12d 17h 55m)
Courtesy of Dr. Asako Sakaue-Sawano and Dr. Atsushi Miyawaki, RIKEN, Japan
(Find more detailed information here.)
Product Selection Guide
| micro-Insert 4 Well FulTrac  Single cells20x Objective lens for full wellcoverageHigh resolution fluorescence microscopy | micro-Insert 4 Well  Few cells4x Objective lens for full wellcoverageHigh resolution fluorescence microscopy |
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Cross Section
Ideal Coverage Using Digital Imaging with CCD Cameras
Possible Filling Procedures:
Minor Well Filling
- Minimal volume
- Individual wells
Whole Insert Filling
- Moderate volume
- Wells connected by medium
Whole µ-Dish Filling
- Maximum volume
- Maximum experimental duration
Evaporation Barrier
More Information
The micro-Insert 4 Well FulTrac was developed in cooperation with the RIKEN Brain Science Institute, Japan.
Reference Publication:
Sakaue-Sawano A, Hoshida T, Yo M, Takahashi R, Ohtawa K, Arai T, Takahashi E, Noda S, Miyoshi H and Miyawaki A. Visualizing developmentally programmed endoreplication in mammals using ubiquitin oscillators. The Company of Biologists Ltd | Development (2013) 140, 4624-4632
http://dev.biologists.org/content/develop/140/22/4624.full.pdf
Stem Cell Differentiation Assays
ibidi has developed several products for long-term cell cultivation of up to several weeks. Constant cell culture conditions are very important for stem cell differentiation studies.
Working with the micro-Insert 4 Well and the micro-Insert 4 Well FulTrac facilitates the handling of just a few initial cells. Using a volume of only 10 µl, the cells can be followed with the microscope for an extended period of time. In combination with our Heating & Incubation System, cell fate determination is possible directly on your microscope.
With ibidi‘s specially designed walls in both, the micro-Insert 4 Well and the micro-Insert 4 Well FulTrac, stem cells can be successfully followed during their differentiation, because the cells cannot “hide” near sharp edges like the wall. The system is long-term stable for days or weeks of stem cell cultivation.
