จานเพาะเลี้ยงเซลล์พร้อมซิลิโคนแบบ 4 ช่อง – สำหรับการถ่ายภาพแบบ live cell ทั้งเซลล์เกาะติดและเซลล์แขวนลอย
- สำหรับการใช้กล้องจุลทรรศน์ในระยะยาว
- การเพาะเลี้ยงเซลล์จำนวนที่น้อยโดยปราศจากการระเหย
- ใน well มีลักษณะคล้ายรูปทรงกรวยเพื่อการมองภาพที่ยอดเยี่ยม
- มีเทคโนโลยี ibiTreat surface เพื่อการเจริญเติบโตของเซลล์ในอุดมคติ
A 4-well silicone insert for live cell imaging of both adherent and suspension cells
- Allows long-term microscopy assays of single cells
- Cultivation of small numbers of cells without evaporation risks
- Conical wells for superb optical quality
- Individual inserts for single experiments
- Ideal cell growth on ibiTreat surface
- Surface Modification: ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized
Pcs./Box: 30 (individually packed)
Product Variation: in µ-Dish 35 mm, high
Applications
- Live cell imaging of adherent and suspension cells
- Immunofluorescence assays
- Immobilization of cells in small wells, e.g., for 3D assays
- Performing long-term microscopy assays lasting up to several weeks
- Co-cultivation of cells
- Cell tracking of very small cell numbers over time
- Cell differentiation, e.g., single stem cells
Specifications
| Number of wells | 4 |
| Dimensions of wells (w x l) in mm | 2.0 x 1.5 (digital format 4:3) |
| Diameter of the complete insert | 12 mm |
| Height of the complete insert | 4.2 mm |
| Volume per well | 10 µl |
| Growth area per well | 0.03 cm2 |
| Coating area per well | 0.23 cm2 |
| Material | Biocompatible silicone |
| Bottom | No bottom – sticky underside |
Technical Features
- Four small 2.0 mm x 1.5 mm microscopy wells with a 4:3 digital format
- Suspension cells cannot escape the observation field
- Adhesive underside
- Transferable to any flat clean surface
- No material is left behind upon the insert’s removal
- Includes a rim for easy handling of the insert with tweezers
Product Selection Guide
| micro-Insert 4 Well FulTrac  Single cells20x Objective lens for full wellcoverageHigh resolution fluorescence microscopy | micro-Insert 4 Well  Few cells4x Objective lens for full wellcoverageHigh resolution fluorescence microscopy |
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Cross Section
Ideal Coverage using Digital Imaging with CCD Cameras
3D Application
Possible Filling Procedures
Minor Well Filling
- Minimal volume
- Individual wells
Whole Insert Filling
- Moderate volume
- Wells connected by medium
Whole µ-Dish Filling
- Maximum volume
- Maximum experimental duration
Evaporation Barrier
Possible Further Applications
Co-Cultivation Assays
Coating patches (spots) with proteins
hydrophilic protein spots on hydrophobic, uncoated µ-Dish
Stem Cell Differentiation Assays
ibidi has developed several products for long-term cell cultivation of up to several weeks. Constant cell culture conditions are very important for stem cell differentiation studies.
Working with the micro-Insert 4 Well and the micro-Insert 4 Well FulTrac facilitates the handling of just a few initial cells. Using a volume of only 10 µl, the cells can be followed with the microscope for an extended period of time. In combination with our Heating & Incubation System, cell fate determination is possible directly on your microscope.
With ibidi‘s specially designed walls in both, the micro-Insert 4 Well and the micro-Insert 4 Well FulTrac, stem cells can be successfully followed during their differentiation, because the cells cannot “hide” near sharp edges like the wall. The system is long-term stable for days or weeks of stem cell cultivation.
