Leica - THUNDER Imager Tissue

กล้องจุลทรรศน์แบบใช้แสงฟลูออเรสเซนต์ พร้อมโปรแกรมประมวลผลภาพแบบ AI ความเร็วสูง ยี่ห้อ ไลก้า รุ่น THUNDER Imager Tissue

โดย Leica THUNDER Imager Tissue ช่วยให้สามารถถ่ายภาพฟลูออเรสเซนต์แบบเรียลไทม์ของตัวอย่างเนื้อเยื่อเช่น ในการวิจัยด้านประสาทวิทยา และเนื้อเยื่อวิทยา โดยสามารถถ่ายภาพได้ออกมาเป็น 3 มิติ และไม่ว่าตัวอย่างจะมีความหนาก็สามารถให้ภาพที่มีรายละเอียดชัดเจน มีความคมชัดและส่วนที่ไม่โฟกัส และฟุ้งเบลอจะถูกกำจัดออกโดยใช้เทคโนโลยี “Computational Clearing” ซึ่งเป็นนวัตกรรมเทคโนโลยีของ Leica โดยกล้องถูกออกแบบมาให้ใช้งานง่าย และมีความรวดเร็วในการถ่ายภาพ ซึ่งเป็นข้อดีของกล้องจุลทรรศน์แบบ Widefield ดังนั้นผู้วิจัยจะได้รับประโยชน์สูงที่สุด และได้รับภาพฟลูออเรสเซนต์ที่มีความคมชัดจากการใช้กล้องจุลทรรศน์ รุ่น THUNDER Imager Tissue

Decode 3D biology in real time*

The THUNDER Imager Tissue allows real-time fluorescence imaging of 3D tissue sections typically used in neuroscience and histology research. Acquire rich, detailed images of thick tissues free of haze from out-of-focus blur.

Even fine structures deep in tissues can be resolved thanks to Computational Clearing, an innovative Leica technology. Image detailed morphological structures like axons and dendrites of neurons in a brain slice. The high image quality, even with thick tissue sections, is combined with the well-known speed, fluorescence efficiency, and ease of use of widefield microscopes.

Gain these advantages using a THUNDER Imager Tissue for your research:

Get computationally cleared images directly in your live preview with THUNDER Live
Rapidly acquire blur-free images showing finest details of the morphology, even deep within thick sections
Get fast overviews of whole tissue sections
Image and analyze challenging tissue sections with an easy workflow

*in accordance with ISO/IEC 2382:2015

Begin every experiment with confidences

Perform high resolution tissue imaging on thick samples without struggling to find your region of interest.

The new THUNDER Live add-on visualizes a computationally cleared image instantly in the live viewer and allows you to optimize ICC parameters by using live image feedback.

Your benefits with THUNDER Live:

Get computationally cleared images directly in your live preview.
Reduce the time to optimal results with immediate visual feedback.
Intuitively and quickly find the best THUNDER ICC parameters on the fly while still in live view.
Optimize your image in one simple step without changing any settings.
THUNDER Live’s blur-free view also makes selecting significant regions of interest in your sample a breeze, even on thick specimens.

Speed up tissue imaging

Increase your slide scanning experiment speed and perform fast and efficient multicolor acquisiton. You’ll be able to assess more markers with less photobleaching.

Your advantages with the accelerated tissue imaging

Scan your slides faster with a synchronized experiment control
Multicolor acquisitions are more than two times faster when using the Synapse controller
Extract contextual information unbelievably fast in slide scanning multicolor experiments
Evaluate more markers through precise excitation control of multiline LED light sources with less photobleaching

Cultured Cortical Neurons, DAPI (cyan) – Nestin cy2 (yellow) – NG2 cy3 (red) – beta III-tubulin Cy5 (magenta). Multiposition acquisitions. Here 4 fluorescence channels are acquired on 6 different positions.

Resolve fine details in challenging specimens

THUNDER Imager Tissue delivers instantly cleared-up fluorescent images with fine details of your multi-color tissue sections. Just turn the THUNDER Imager on and get started! The unique Computational Clearing uses optimal parameters to produce expert-level results. It does it automatically with no need for calibration or user interaction.

Achieve optimal fluorescence and contrast settings instantly via the patented Leica fluorescence intensity (FIM) and contrast managers. Choose from a range of objectives, optimized for specific applications, to ensure outstanding results even for challenging specimens.

Cyclops sp. Nuclei (green), acetylated -tubulin (red), Serotonin (cyan). Image stack of a total volume of 332 x 332 x 84 µm³ with three colors and 305 z planes per color. Objective used was HC PL FLUOTAR 40x/1.30 OIL. Sample courtesy Dipl. Biol. Thomas Frase, University of Rostock, Allgemeine & Spezielle Zoologie, Institut for Biosciences, Rostock (Germany).

Two Options for THUNDER Imager Tissue

Choose the configuration which best meets your requirements:

THUNDER Imager 3D Tissue is a fully automated tissue imaging system for recording multi-color 3D images, now directly in your live preview*. Capture multiple images in the z-direction and visualize them in the 3D viewer. Achieve excellent z-stack imaging due to a precise motorized focus drive.
For brilliant imaging of single planes and fast overviews of your tissue the THUNDER Imager Tissue is also available in a cost-effective configuration without a motorized focus drive.

*Only available with THUNDER Imager 3D Tissue

Combination System: LMD System with THUNDER Imaging

THUNDER can be combined with additional Laser Microdissection (LMD) capabilities in the same system. The base stand of the LMD systems and THUNDER Imager 3D Tissue are the same, thus such a combination offers:

Lab space savings with one system for different tasks
Brilliant fluorescence imaging with THUNDER using LAS X
Laser Microdissection with the LMD option using the unique LMD Software to visualize and mark regions of interest for subsequent dissection and collection via gravity into standard vials ready for downstream processing such as RNAseq, NGS, MS, qPCR, microarray etc.

Leica LMD principle: Tissue sections are mounted on a special membrane. A UV-laser cuts according pre-defined markings and dissectates drop into collections wells beneath the slide.

THUNDER Imager TissueBrilliant imaging of single planes and fast overviews	THUNDER Imager 3D TissueFully automated tissue imaging system for recording multi-color 3D images
Microscope Platform	DM4 B	DM6 B
Display/Touch screen	Display shows all microscope settings at a glance	SmartTouch controls the microscope conveniently and intuitively
Focus	Mechanical	Motorized
Stage	Motorized with integrated controller, mechanical (optional)	Motorized or scanning stages
LAS X Navigator Software	Yes (optional)	Yes
Transmitted Light Illumination	LED	LED
Transmitted Light Contrast Methods	BF, optional PH, DF, POL, DIC	BF, optional PH, DF, POL, DIC
Fluorescence Axis	Fully automated	Fully automated, with Internal Filter Wheel (IFW) and Excitation Manager
Fluorescence Illumination	EL6000 (metal halide), LED	LED, EL6000 (optional)