Chamber slide แบบ 8 ช่อง มี Grid ขนาด 500 ไมครอนสำหรับหาตำแหน่งเซลล์ – สำหรับงาน immunofluorescence หรือการถ่ายภาพความละเอียดสูง
- เหมาะสำหรับการหาตำแหน่งของเซลล์
- ก้นภาชนะมีความบางเหมือนกับ Coverslip สามารถใช้ในการถ่ายภาพความละเอียดสูงได้
- ใช้เซลล์และ Reagent ในปริมาณน้อย ช่วยประหยัดค่าใช้จ่ายในการทำการทดลอง
A chambered coverslip with 8 individual wells, high walls, and an imprinted 500 µm cell location grid for use in immunofluorescence and high-end microscopy
- Ideal for locating cells or cell clusters
- Observation of the sample through the polymer coverslip bottom using high resolution microscopy
- Cost-effective experiments using small numbers of cells and low volumes of reagents
- Extra high individual walls to keep cross contamination between wells as low as possible
- Surface Modification: ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized
Pcs./Box: 15 (individually packed)
Grid: Grid-500
Applications
- Locating cells or cell clusters, e.g., transfected cells for clone picking
- Counting events per defined area (e.g., for calculating transfection efficiency)
- Providing a reference structure for cell movements
- Cultivation and microscopy of cell cultures
- Transfection assays
- Fluorescence microscopy of living and fixed cells
- Live cell imaging over extended time periods
- Suitable for DIC, when used with a DIC lid
Specifications
Geometry of the Grid-500
| Number of view fields | 100 |
| Repeat distance | 500 µm (+/- 1%) |
| Groove width | 5 µm +/- 1 µm |
| Groove depth | < 5 µm |
Geometry of the µ-Slide 8 Well high
| Outer dimensions (w x l) | 25.5 x 75.5 mm² |
| Number of wells | 8 |
| Dimensions of wells (w x l x h) | 9.4 x 10.7 x 9.3 mm³ |
| Volume per well | 300 µl |
| Total height with/without lid | 10.8/9.5 mm |
| Growth area per well | 1.0 cm² |
| Coating area per well | 2.2 cm² |
| Bottom: ibidi Polymer Coverslip | |
Technical Drawing
Technical drawings and details are available in the Instructions (PDF).
Technical Features
- Grid with a 500 µm repeat distance
- Lettered and numbered fields (A–K; 1–10)
- Open µ-Slide (chambered coverslip) with 8 independent wells
- Individual well walls for minimizing well-to-well crosstalk and contaminations
- Grid clearly visible in phase contrast and bright field microscopy; slightly visible in fluorescence mode
- Excellent optical quality imaging chamber for high-end microscopy
- Compatible with staining and fixation
- ibiTreat surface for optimal cell adhesion
- Biocompatible plastic material—no glue, no leaking
Layout Grid-500
