ibidi : µ-Slide 4 Well Ph+ - Chamber slide แบบ 4 ช่อง สำหรับงาน Phase contrast

Chamber slide แบบ 4 ช่อง – ประกอบด้วย intermediate plate เหมาะสำหรับเทคนิคแสงแบบ Phase contrast และการถ่ายภาพความละเอียดสูง

เหมาะสำหรับ Phase contrast โดยไม่ทำให้เกิด meniscus
แบบ All-in-one chamber slide สำหรับการเพาะเลี้ยงเซลล์ และการถ่ายภาพด้วยกล้องจุลทรรศน์
ก้นภาชนะมีความบางเหมือนกับ Coverslip สามารถใช้ในการถ่ายภาพความละเอียดสูงได้
สามารถทำ immunofluorescence ได้รวดเร็วและง่าย โดยไม่มี coverslips และไม่มีการรั่วซึม

A chambered coverslip with 4 wells and a special intermediate plate for excellent phase contrast and high-end fluorescence microscopy

Excellent phase contrast over the entire well–no meniscus
All-in-one chamber slide used for cell culture and microscopy
Observation of the sample through the polymer coverslip bottom using high resolution microscopy
Fast and easy immunofluorescence, with no coverslips and no leakage
Surface Modification: ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized
Pcs./Box: 15 (individually packed)

Applications

Cultivation and microscopy of cell cultures
Transfection assays
Fluorescence microscopy of both living and fixed cells
Live cell imaging over extended time periods

Want to know if you should use a glass or a polymer bottom for your application? Find out here.

Phase contrast microscopy is the most commonly used, transmitted light technique in cell culture. When working with phase contrast microscopy, it is crucial to have the two phase rings adjusted to each other. In open wells, the meniscus at the air-water-interphase works like a lens that refracts the beam path. This miscalibrates the phase rings, leading to poor contrast in the microscopic image.

Working with the µ-Slide 4 Well^Ph+ diminishes the meniscus, so that the whole optical system is aligned, no matter which position of the well is being imaged.

Specifications

Outer dimensions (w x l)	25.5 x 75.5 mm²
Number of wells	4
Dimensions of wells (w x l x h)	21.6 x 11.4 x 9.3 mm³
Volume per well	700 µl
Liquid height	3.0 mm
Total height with / without lid	10.8 / 9.5 mm
Growth area per well	2.5 cm²
Coating area per well	5.9 cm²
Bottom	ibidi Polymer Coverslip

Technical Features

Open µ-Slide with 4 independent wells
Intermediate plate in each well to avoid meniscus formation
Easy filling, without air bubbles, through the lateral slot
Excellent optical quality for high end microscopy
Ph+ feature for perfect phase contrast—no meniscus formation
Compatible with staining and fixation
ibiTreat surface for optimal cell adhesion
Made of biocompatible plastic material – no glue and no leaking
Also available with a glass bottom

Experimental Example

Meniscus-Free Phase Contrast Microscopy

The µ-Slide 4 Well^Ph+ (Phase Contrast +) is designed for excellent optical quality, especially for cell culture when normal phase contrast microscopy is being used. Opposed to the classic µ-Slide 4 Well, the Ph+ version provides a special intermediate plate in the center of the well. This plate flattens the meniscus that disturbs the phase contrast effect in normal open wells. Openings near the corners provide access to the wells for easy filling and aspiration of liquids.

This innovative technique supports meniscus-free phase contrast microscopy in a very convenient manner.

Working with the µ-Slide 4 Well^Ph+ diminishes the meniscus and increases the area of well contrasted cells

The illustration on the left shows the perturbing effect of a meniscus. Light is refracted on the air-water-interface, leading to poor contrast in microscopy. Only the small center part exhibits satisfying phase contrast.

Working with the µ-Slide 4 Well^Ph+ diminishes the meniscus and increases the area of nicely contrasted cells. This nice contrast is due to the parallel beam path that is created by the plate.

Comparison Ph+ Well versus Standard Well: No Meniscus Effect in Ph+ Well

Standard Well

Poor Phase Contrast
Excellent Fluorescence Microscopy

µ-Slide 4 Well^Ph+

Excellent Phase Contrast
Excellent Fluorescence Microscopy