ibidi : sticky-Slide Chemotaxis

สไลด์แบบ chemotaxis แบบไม่มีก้น โดยด้านล่างมีกาวในตัวซึ่งสามารถติดที่พื้นผิวได้

อเนกประสงค์ โดยสามารถใช้ติดกับวัสดุที่หลากหลาย
สำหรับการวัด chemotaxis แบบ real time
มีความสเถียรสำหรับการทดลองในระยะยาว

A chemotaxis bottomless slide used in 2D and 3D applications with a self-adhesive underside designed to allow self-assemblage of the slide setup

Versatile – i.e., permits the mounting of a variety of bottom materials
Allows chemotaxis measurements to be taken in real-time
Provides stable gradients for long-term experiments
Pcs./Box: 10 (individually packed)

Applications:

Inserting materials or tissue into chemotaxis chambers
Keeping cell spheroids or tissue in a stable concentration gradient
Chemotaxis of fast or slow migrating cells on either a 2D surface or in 3D gels
Usable with a range of bottom materials, such as plastic sheets, silicon chips, and glass slides

Specifications:

Outer dimensions (w x l)	25.5 x 75.5 mm²
Chemotaxis chambers on slide	3
Volume per chamber	130 µl
Observation area	2 x 1 mm²
Height of reservoirs	1.05 mm
Total height with plugs	12 mm
Volume chemoattractant	30 µl
Bottom	none

Applications:

Inserting materials or tissue into chemotaxis chambers
Keeping cell spheroids or tissue in a stable concentration gradient
Chemotaxis of fast or slow migrating cells on either a 2D surface or in 3D gels
Usable with a range of bottom materials, such as plastic sheets, silicon chips, and glass slides

Specifications:

Outer dimensions (w x l)	25.5 x 75.5 mm²
Chemotaxis chambers on slide	3
Volume per chamber	130 µl
Observation area	2 x 1 mm²
Height of reservoirs	1.05 mm
Total height with plugs	12 mm
Volume chemoattractant	30 µl
Bottom	none

Technical Features:

Bottomless slide
Self-adhesive underside
Biocompatible adhesive that has been cell culture tested
Adheres to all flat surfaces, even wet surfaces
Suitable coverslips available
Specifications identical to those of the µ-Slide Chemotaxis, except that it has no bottom

Placing Tissue Sample in the sticky-Slide Chemotaxis

Application Example: Directed Invasion of MCF-7 Cancer Cells in an FCS Gradient

This movie shows the invasion of MCF-7 cancer cells into a collagen gel during 48 hours. The center channel of a sticky-Slide Chemotaxis was increased in height by milling in order to position the MCF-7 spheroid. The left side contains 10% fetal calf serum, the right side is serum-free. Phase contrast microscopy, objective lens 4x.