Leica - The Power HyD Detector Family ชุดรับสัญญาณ Confocal ที่มีความไวสูงสุด

Leica - The Power HyD Detector Family

Leica - The Power HyD Detector Family

ความไวแสงระดับ Super Sensitivity ด้วย HyD (Hybrid Detector) ยี่ห้อ ไลก้า



ความไวแสงระดับ Super Sensitivity ด้วย HyD (Hybrid Detector)

การรับสัญญาณด้วยความไวระดับการนับ Photon? ไม่ว่าจะสัญญาณน้อยหรือมาก? หรือต้องการถ่ายภาพความเร็วสูงและคมชัด? ด้วย HyD เราสามารถทำทุกอย่างพร้อมกัน ซึ่งสัญญาณที่ได้จะได้ภาพที่มี Contrast และสัญญาณที่ยอดเยี่ยม ได้สัญญาณที่พร้อมสำหรับตีพิมพ์ลงวรสาร HyD นี้จะเป็นมาตรฐานใหม่ของการถ่ายภาพ Confocal ในอนาคต

Our confocal detectors provide improved photon counting, ultra-sensitive imaging and more color options in the near-infrared spectrum


With STELLARIS, we have pushed the limits of our detector technology, so you can push the boundaries of your research. Our Power HyD detector family consists of three different types of detectors designed to fit your confocal applications.





With the STELLARIS confocal platform, we have re-imagined confocal microscopy to get you closer to the truth.

Three detectors provide improved photon counting

At the heart of the STELLARIS confocal platform are our core Power HyD S detectors. These detectors offer both analog and photon counting detection capabilities in confocal imaging.

The Power HyD R detectors deliver optimized imaging in the near-infrared , while our Power HyD X are specialized detectors for FLIM and fluorescence correlation spectroscopy ( FCS ) imaging. Both Power HyD R and Power HyD X offer photon counting and digital modes and are options to extend the application flexibility in STELLARIS 8.

The STELLARIS Power HyD detector family delivers the highest sensitivity in a wide spectral range. The powerful confocal detectors provide detection flexibility for a broad range of application needs, enabling you to obtain brilliant, publication-ready images .

Improved photon counting capacities

Conventional photon counting works accurately at low signal intensities, when photons are hitting the detector sparsely, but cannot differentiate between one or two photons hitting the detector at the same time. Leica Microsystems has implemented a new proprietary approach to photon counting, Power Counting, that overcomes this limitation and is capable of distinguishing overlapping photons from a single photon. The entire Power HyD detector family is equipped with this new approach.

Our Power HyD S technology in combination with Power Counting delivers more than twice dynamic range* compared with classical photon counting. This enhanced capacity to count photons improves image contrast, especially at high signal intensities where the likelihood of having overlapping photons is much greater. Now you can obtain quantitative results, thanks to Leica Microsystems’ proprietary approach to photon counting.

*comparison of linear range of STELLARIS HyD S vs SP8 HyD in photon counting mode (CW)

HeLa cells labeled with WGA-CF488 dye.
HeLa cells labeled with WGA-CF488 dye. Left: Conventional photon counting result; right: Power Counting result. Shown are maximum-intensity-projections of Z-stacks acquired with 12kHz resonant scanner, processed by LIGHTNING

Two detection modalities, zero compromise for imaging

The Power HyD S detectors are the new core of STELLARIS. These silicon-based Multi-Pixel Photon Counter (MPPC) detectors are the result of years of engineering experience at Leica Microsystems. The result is enhanced image quality in confocal imaging.

The Power HyD S offer both analog and photon counting operating modes, providing unique flexibility and versatility for a wide range of confocal applications. The analog detection delivers the highest dynamic range , which allows you to collect accurate and quantitative data at either high or low photon counts. By switching to the photon counting mode, you are provided with ultra-low dark noise giving you the sensitivity to even the faintest of signals .

Illustration Detectors
Left: Schematic showing the multi-cell architecture of the Power HyD S detector. Right: Processing of detector signals in the two available operating modes, photon counting and analog.

Ultra-sensitive image detection: Preserve sample integrity through efficient signal acquisition

The Power HyD S detectors provide high contrast images thanks to improved sensitivity and low dark noise. This combination is especially useful for delicate samples that need to be imaged under low laser power. High sensitivity and low background signal mean that delicate samples can still be imaged clearly, with almost no interference or noise even with the laser power reduced to a minimum, protecting them from photodamage.

The Power HyD S detectors allow for enhanced sensitivity in the blue-green range with a Photon Detection Efficiency (PDE) of up to 56%, more than double compared to conventional multi-alkali photomultiplier tube (PMT) detectors. This ultra-sensitive detection comes in the range where most commonly used fluorescent probes such as CFP or GFP emit, helping you improve your live cell imaging experiments, even at low level signals.

3D image acquisition with
Zebrafish embryo. 3D image acquisition with STELLARIS 8 equipped with Power HyD S (Nuclei: blue, Hoechst), Power HyD X (Endothelial Cells: green, EGFP); and Power HyD R detectors (Cell membranes: magenta, NIR750). Sample courtesy of Julien Vermot, IGBMC, Strasbourg.

Extended red range imaging with STELLARIS

The Power HyD detector family, in combination with the new extended white light laser, provides more color options in the near-infrared spectrum, delivering an incredibly wide spectral range for excitation and detection of fluorescence.

The Power HyD detector family extends the detection range out to 850 nm , allowing you to add up to 3 additional fluorophores to your experiment . This new versatility gives you more options when it comes to including Near Infrared Dyes in your experiments.

The Power HyD S and Power HyD R detectors give excellent sensitivity in the Near Infrared spectral range*, with at least 2x higher PDE compared to PMT detectors . Our Power HyD R detectors for extended red performance give you excellent signal in this range, and optimal use of the new WLL wavelengths allows for optimal excitation for several key fluorophores in the new rage (e.g. AF750, AF790, CF 700, CF 750, CF 770 ).

* Near Infrared spectral range (720-850nm).

Images of Cos-7 cells
Images of Cos-7 cells, labeled with SiR-Actin (657 – 740 nm detection range), AF750-Tom20 (760 – 790 nm), AF790-Tubulin (810 – 850 nm). Sample courtesy of Jana Döhner, Urs Ziegler, University of Zürich. Left: Seen by conventional GaAsP detectors. Right: Seen by STELLARIS 8.

Unleash the potential of STELLARIS with TauSense

By providing high-speed photon counting with accurate photon arrival time information, all the Power HyD detectors enable the use of TauSense, allowing for the simultaneous capture of fluorescence intensity and lifetime-based information from the dyes in your sample . TauSense is completely integrated so that, at the push of a button, it is possible to add an extra dimension of information with just one click. TauSense enables you to explore the cellular microenvironment and metabolic states as well as separate dyes based on their fluorescence lifetime information.

Arabidopsis Thaliana leaf with stomatum.
Arabidopsis Thaliana leaf with stomatum. Actin: Red, Lifeact-Venus (Era et al.Plant Cell Physiol., 2009); chloroplasts: Green, endogenous fluorescence. Image acquired with TauContrast. Sample courtesy of Dr. Melanie Krebs, COS, University of Heidelberg.

Specialized detectors for FLIM, FCS, FLCS

The Power HyD X and Power HyD R detectors are especially optimized to make the most of fast lifetime imaging applications. To detect single molecules as is often required in lifetime imaging applications, extreme sensitivity is required from the microscope detector. The Power HyD X and Power HyD R detectors have been developed to be the perfect detector for all SMD (single molecule detection) methods. An active cooling system comprised of built-in Peltier cooling and additional external cooling that reduces dark noise , result in the highest SMD data quality. High quantum efficiency combined with virtually no detector after-pulsing allows acquisition of precise diffusion data. Thanks to high photon count numbers and short instrument response time , the Power HyD X and Power HyD R detectors are world leading for use in lifetime applications.

Calcium oscillations after stimulation with thrombin activating peptide. The response in individual cells is recorded as a change in lifetime. Movie acquired at 4 pfs. Image size: 256 x 256 pixels. Color bar scale (lifetime): ns. Courtesy Kees Jalink, Bram van den Broek, NKI Amsterdam

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