ibidi : µ-Slide 8 Well Grid-500
A chambered coverslip with 8 wells and an imprinted 500 µm cell location grid for use in immunofluorescence and high-end microscopy
- Ideal for locating cells or cell clusters
- Observation of the sample through the polymer coverslip bottom using high resolution microscopy
- Cost-effective experiments using small numbers of cells and low volumes of reagents
- Surface Modification: ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized
Pcs./Box: 15 (individually packed)
Grid: Grid-500
Applications
- Locating cells or cell clusters, e.g., transfected cells for clone picking
- Counting events per defined area (e.g., for calculating transfection efficiency)
- Providing a reference structure for cell movements
- Cultivation and microscopy of cell cultures
- Transfection assays
- Fluorescence microscopy of living and fixed cells
- Live cell imaging over extended time periods
- Suitable for DIC, when used with a DIC lid
Specifications
Geometry of the Grid-500
| Number of view fields | 100 |
| Repeat distance | 500 µm (+/- 1 %) |
| Groove width | 5 µm (+/- 1 µm) |
| Groove depth | < 5 µm |
Geometry of the µ-Slide 8 Well
| Outer dimensions (w x l) | 25.5 x 75.5 mm² |
| Number of wells | 8 |
| Dimensions of wells (w x l x h) | 9.4 x 10.7 x 6.8 mm³ |
| Volume per well | 300 µl |
| Total height with lid | 8 mm |
| Growth area per well | 1.0 cm² |
| Coating area per well | 2.20 cm² |
| Bottom: ibidi Polymer Coverslip | |
Technical Features
- Grid with a 500 µm repeat distance
- Now also available as a µ-Slide 8 Well high Grid-500 with extra high individual walls to keep cross contamination between wells as low as possible
- Lettered and numbered fields (A-K; 1-10)
- Open µ-Slide (chambered coverslip) with 8 independent wells
- Grid clearly visible in phase contrast and bright field microscopy; slightly visible in fluorescence mode
- Excellent optical quality imaging chamber for high-end microscopy
- Compatible with staining and fixation
- ibiTreat surface for optimal cell adhesion
- Biocompatible plastic material — no glue, no leaking
Layout Grid-500
