ibidi : µ-Plate 96 Well Black Glass Bottom

ไมโครเวลเพลท 96 หลุมแบบสี่เหลี่ยม สีดำ ก้นทำจากแก้ว – สำหรับงานเพาะเลี้ยงเซลล์

A black 96 well plate with square wells and a #1.5H glass coverslip bottom for high throughput screening using TIRF and super-resolution microscopy

A microplate ideal for high-end cell microscopy due to black walls and a thin glass coverslip bottom
Brilliant cell imaging thanks to the low thickness variability of the #1.5H glass coverslip and the excellent inner well and whole plate flatness
Low well-to-well crosstalk in fluorescence microscopy
Surface Modification: #1.5H (170 µm +/- 5 µm) D 263 M Schott glass, sterilized
Pcs./Box: 15 (individually packed)

Applications

Cultivation, high throughput screening (HTS), and high-resolution microscopy of cells
Widefield fluorescence imaging and confocal microscopy of living and fixed cells
Compound screenings (toxicology)
Large-scale transfection experiments
Live cell imaging, also suited for extended periods
Total internal reflection fluorescence (TIRF) and single molecule applications
Super-resolution microscopy (STED, SIM, (F)PALM, (d)STORM) and fluorescence correlation spectroscopy (FCS)
Immunofluorescence staining

Want to know if you should use a glass or a polymer bottom for your application? Find out here.

Specifications

Length / width	127.8 / 85.5 mm
Height with / without lid	17.2 / 15.0 mm
Well-to-well distance	9.0 mm
Well clearance	0.73 mm
Single well dimensions	7.4 x 7.4 mm²
Single well depth	13.0 mm
Volume per well	300 µl
Growth area per well	0.56 cm²
Coating area per well using 300 µl	2.35 cm²
Bottom: Glass Coverslip No. 1.5H, selected quality, 170 µm +/- 5 µm

Technical Drawing

Technical Features

Compatible with robotics and plate readers due to an ANSI/SLAS (SBS) standard format (85.5 x 127.5 mm)
96 quadratic wells with standard numbering (letters A–H and numbers 1–12)
Suitable for fluorescence scanners
Bottom made from D 263 M Schott glass with a thickness of 170 µm +/- 5 µm
May require coating to promote cell attachment
No autofluorescence
Excellent inner well flatness and whole plate flatness
Compatible with staining and fixation solvents
Sterile with single packaging
Also available as µ-Plate 96 Well Black with a #1.5 ibidi Polymer Coverslip Bottom for optimized cell adhesion—for everyday use with diverse application possibilities

The Principle of the µ-Plate 96 Well Black Glass Bottom

The Coverslip Bottom

The µ-Plate 96 Well Black Glass Bottom comes with a high precision D263 M Schott glass, No 1.5H (170 +/– 5 µm) Glass Coverslip bottom. This bottom is ideally suitable for high-resolution microscopy and special microscopic applications like TIRF or super-resolution.

Find more information about the different surfaces of the ibidi chambers here.

Application Examples

Fluorescence microscopy of immunostained Rat1 fibroblasts in the µ-Plate 96 Well Black Glass Bottom. Cells were stained for F-actin (red), Talin (green) and nuclei (DAPI, blue) and imaged using a 60x objective.

Fluorescence microscopy of immunostained Rat1 fibroblasts in the µ-Plate 96 Well Black Glass Bottom. Cells were stained for F-actin (red), alpha-Tubulin (green) and nuclei (DAPI, blue) and imaged using a 60x objective..