ibidi : µ-Dish 35 mm, high

ibidi : µ-Dish 35 mm, high – จานเพาะเลี้ยงเซลล์ สำหรับการถ่ายภาพ ขนาด 35 มม. (ขอบสูง)

จานเพาะเลี้ยงเซลล์ แบบก้นบางพิเศษ แบบ polymer coverslip สำหรับการถ่ายภาพ เส้นผ่านศูนย์กลางขนาด 35 มม. – ให้ภาพความละเอียดสูง เหมาะสำหรับยึดติด หรือ Adherence cell

มีเทคโนโลยี ibiTreat surface เพื่อการเจริญเติบโตของเซลล์ มีฝาปิด พร้อมตำแหน่งล็อคที่ขอบจาน ช่วยลดการระเหยเหมาะสำหรับเทคนิค DIC เมื่อใช้ร่วมกับฝาปิดพิเศษสำหรับเทคนิค DIC

A 35 mm imaging dish with a polymer coverslip bottom for high-end microscopy and cell-based assays

In this microscopy dish, the cells are imaged on a No. 1.5 ibidi Polymer Coverslip bottom with the highest optical quality and optimal cell adherence
Cell culture petri dish for everyday use with diverse application possibilities; equipped with a lid with locking feature that minimizes evaporation
Suitable for DIC when using a DIC Lid
Surface Modification: Uncoated: #1.5 polymer coverslip, hydrophobic, sterilized
Pcs./Box: 60 (individually packed)
Surface Modification: ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized
Pcs./Box: 400 (individually packed)

Applications

Cultivation and high-resolution imaging of cells
Immunofluorescence staining
Widefield and confocal fluorescence microscopy of living and fixed cells
Live cell imaging over extended time periods
Transfection assays
Differential interference contrast (DIC) when using a DIC Lid
Cell location and counting when using the gridded version

Want to know if you should use a glass or a polymer bottom for your application? Find out here.

Specifications

Ø µ-Dish	35 mm
Volume	2 ml
Growth area	3.5 cm²
Coating area using 400 µl	4.1 cm²
Ø observation area	21 mm
Height with / without lid	14/12 mm
Bottom: ibidi Polymer Coverslip

Technical Features

Standard format imaging dish with a 35 mm diameter for tissue culture
ibiTreat (tissue culture-treated) surface for optimal cell adhesion and cell growth
High walls with a standard height for easy handling; low wall version available: µ-Dish^{35 mm, low}
Lid with locking feature for minimal evaporation
Rim for easy opening
No autofluorescence
Biocompatible plastic material—no glue, no leaking
Available as a Bulk Pack with 400 individually packed µ-Dishes per box
Available with a #1.5H Glass Coverslip Bottom: µ-Dish^{35 mm, high} Glass Bottom, specifically suitable for TIRF and single molecule applications
Available with a 500 µm grid for cell counting: µ-Dish^{35 mm, high} Grid-500
Available with a non-adhesive Bioinert surface: µ-Dish^{35 mm, high} Bioinert, specifically suitable for 3D applications, such as spheroids and suspension cells

Test our everyday solution for cost-effective high-throughput experiments: Glass Bottom Dish^{35 mm}

The Principle of the µ-Dish^{35 mm, high}

The Coverslip Bottom

The µ-Dish^{35 mm, high} comes with a thin ibidi Polymer Coverslip Bottom that has the highest optical quality (comparable to glass) and is ideally suitable for high-resolution microscopy. It is also available with a #1.5H Glass Coverslip Bottom for TIRF or single molecule microscopy.

As another option for cost-effective high-throughput experiments, we offer the Glass Bottom Dish^{35 mm} with a #1.5 glass coverslip bottom.

Find more information and technical details about the coverslip bottom of the ibidi chambers here.
Find a comparison of the different ibidi Dishes^{35 mm} here.

The ibiTreat Surface

ibiTreat (tissue culture-treated) is our most recommended surface modification, because almost all adherent cells grow well on it without the need for any additional coating.

Find more information about the different surfaces of the ibidi chambers here.

Lid with Locking Feature for Minimized Evaporation

All ibidi µ-Dishes are equipped with the special lid-locking feature. The locking position minimizes evaporation, and thereby provides excellent conditions for long-term studies in a non-humidified environment. Gas exchange (carbon dioxide or oxygen) during cell culture is maintained thanks to the gas-permeable plastic material of the dish.

TIP: Use the locking feature if minimal evaporation is required (e.g., outside incubators, non-humidified microscopy stages, etc.).

Experimental Examples

Differentiated PC12 cells in a μ-Dish^{35 mm, high}, stained for ß-III-Tubulin (Covance, Princeton, USA) in red. PC12 cells were incubated with commercial green-fluorescent magnetic nanoparticles (MAG-ARA, Chemicell, Berlin) and nuclei were stained with 4‘, 6-diamidino-2- phenylindole (DAPI, Roche Applied Systems, Indianapolis, USA). Image by Josephine Pinkernelle, Otto-Von-Guericke University Magdeburg, Magdeburg, Germany.

Ultra-structure of Streptococcus pyogenes biofilm grown on mouse embryonic fibroblasts in a μ-Dish^{35 mm, high} ibiTreat. Blue – Phalloidin AF350; Green – anti-Streptococcus pyogenes primary antibody, Rabbit anti-goat AF488 secondary antibody; Red – WGA AF594. Image by Anuradha Vajjala, Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, Singapore.

Brightfield and fluorescence images of multicellular shapes created using Biopixlar

Brightfield and fluorescence images of multicellular shapes created in the μ-Dish^{35 mm, high} using Biopixlar, a bioprinter capable of generating multicellular biological tissues directly in the cell culture media. Images by Fluicell.

Live rat kidney cells stained with Di-8-ANEPPS to image primary cilia. Cells were plated on a μ-Dish and imaged on a Nikon A1R inverted confocal microscope with a 60X NA 1.27 WI objective using 457nm excitation and a 490nm LP filter to the detection PMT. 3D volume rendering has been depth coded with NIS Elements software. Image by Brian Siroky, Alyssa Wilson, and Matthew Kofron, Cinncinnati Children’s Hospital, Cincinnati, OH, USA.

ibidi µ-Dish Height Variations

µ-Dish^{35 mm, high}

High walls for all standard cell culture applications

µ-Dish^{35 mm, low}

Low walls that provide greater cell access, which is useful for micromanipulation

Triple immunofluorescence of bovine endothelial cells.
Red: mitochondria, stained with MitoTracker™ Red CMXRos;
Green: F-actin, stained with Alexa Fluor™ 488 Phalloidin;
Blue: nuclei, stained with DAPI.

Comparison of the ibidi Dishes^{35 mm}

Please find a detailed comparison of the material specifications, including suitable microscopy applications for the ibidi Polymer Coverslip, the ibidi Glass Bottom, and further materials here.

	µ-Dish^{35 mm, high}	µ-Dish^{35 mm, high} Glass Bottom	Glass Bottom Dish^{35 mm}
Bottom material	#1.5 ibidi Polymer Coverslip	#1.5H ibidi Glass Coverslip	#1.5 glass coverslip
Bottom thickness	180 µm (+10/-5 µm)	170 µm (+/-5 µm)	170 µm (+20/-10 µm)
Bottom: gas permeability	Yes	No	No
Available surfaces	ibiTreat (tissue culture treated), Uncoated (hydrophobic)	Uncoated glass	Uncoated glass
Lid	Lid with locking feature	Lid with locking feature	Standard lid
Packaging	Sterile, individually packed	Sterile, individually packed	Sterile, 10 pcs. per case
Quantity	60 or 400 pcs./box	60 or 400 pcs./box	200 or 800 pcs./box
Low wall version available?	Yes: µ-Dish^{35 mm, low}	Yes: µ-Dish^{35 mm, low} Glass Bottom	No
Gridded version available?	Yes: µ-Dish^{35 mm, high} Grid-500	Yes: µ-Dish^{35 mm, high} Grid-50 Glass Bottom, µ-Dish^{35 mm, high} Grid-500 Glass Bottom	No