ibidi : Perfusion Set

ชุดท่อพร้อมใช้สำหรับการทดสอบการไหลของของเหลว ด้วยระบบ ibidi : Pump System

• รวมท่อและแหล่งกักเก็บเพื่อทำการทดสอบการไหลร่วมกับสไลด์ช่องสัญญาณ ibidi และหน่วยของเหลว (ibidi : Channel Slides และ ibidi : Fluidic Unit)
• ออกแบบมาสำหรับอัตราการไหลและปริมาตรต่างๆ
• ปลอดเชื้อและพร้อมใช้งาน

Ready-to-use tubing sets for flow assays with the ibidi Pump System

• Includes tubing and reservoirs to perform flow assays in combination with the ibidi Channel Slides and the Fluidic Unit
• Designed for various flow rates and volumes
• Sterile and ready-to-use

For detailed information on the complete ibidi Pump System, please refer to the ibidi Pump System website.

your selections

• Product Variation: BLUE; length 15 cm, ID 0.8 mm, 10 ml reservoirs Pcs./Box: 4 (individually packed)
• Product Variation: WHITE; length 50 cm, ID 0.8 mm, 10 ml reservoirs Pcs./Box: 4 (individually packed)
• Product Variation: WHITE; length 50 cm, ID 0.8 mm, 10 ml reservoirs Pcs./Box: 4 (individually packed)
• Product Variation: YELLOW; length 15 cm, ID 0.5 mm, 2 ml reservoirs Pcs./Box: 4 (individually packed)
• Product Variation: BLACK; length 50 cm, ID 0.5 mm, 2 ml reservoirs Pcs./Box: 4 (individually packed)
• Product Variation: RED; length 15 cm, ID 1.6 mm, 10 ml reservoirs Pcs./Box: 4 (individually packed)
• Pcs./Box: 4 (individually packed) Product Variation: BROWN; length 100 cm, ID 0.5 mm, 2 ml reservoirs
• Pcs./Box: 4 (individually packed) Product Variation: GREY; length 100 cm, ID 0.8 mm, 10 ml reservoirs
• Pcs./Box: 4 (individually packed) Product Variation: ORANGE; length 100 cm, ID 1.6 mm, 10 ml reservoirs

Perfusion Sets and µ-Slide Selection

The possible shear stress range achieved with the ibidi Pump System depends on the combination of the respective Perfusion Set and µ-Slide in use.

Selection Guide

Find the optimal Perfusion Set for your experiment depending on your desired shear stress range and µ-Slide combination:

The given shear stress ranges are valid for the viscosity of standard medium with 10% FCS at 37°C (0.0072 dyn s/cm²). The minimal value refers to a pressure of 5 mbar and the maximal value refers to a pressure of 95 mbar.

Overview

	Red	Yellow/Green	Orange	Blue	White	Grey	Yellow	Black	Brown
Inner diameter (ID, mm)	1.6	1.6	1.6	0.8	0.8	0.8	0.5	0.5	0.5
Tube length (cm)	15	50	100	15	50	100	15	50	100
Working volume (ml)	12.6	13.3	14.3	12.4	12.6	12.9	2.5	2.6	2.7
Standard reservoir* (ml)	10	10	10	10	10	10	2	2	2

* By changing the reservoir, different working volumes can be achieved. Reservoirs are available with a syringe volume of 2, 10, and 50 ml.

Details

NOTE: The given shear stress ranges are valid for the viscosity of standard medium with 10% FCS at 37°C (0.0072 dyn s/cm²).

NOTE: The given flow rate and shear stress ranges are only valid when using one Fluidic Unit with one single connected slide. If several Fluidic Units or several slides are connected, the range shifts towards lower values.

	Perfusion Set red (#10962)		Inner diameter: 1.6 mm Total working volume: 12.6 ml Tube length: 15 cm		Dead volume of tubing: 0.3 ml Viscosity 0.0072 dyn s/cm²
	Growth area [cm²]	Channel volume [µl]	Flow rate [ml/min]		Shear stress [dyn/cm²]
			MIN	MAX	MIN	MAX
µ-Slide I 0.2 Luer	2.5	50	2.0	27.0	7.5	95.0
µ-Slide I 0.2 Luer Glass	2.5	62.5	3.1	36.0	7.5	82.0
µ-Slide I 0.4 Luer	2.5	100	4.2	47.0	4.0	43.0
µ-Slide I 0.4 Luer Glass	2.5	112.5	4.6	52.0	3.5	38.0
µ-Slide I 0.6 Luer	2.5	150	4.5	49.0	2.0	20.0
µ-Slide I 0.6 Luer Glass	2.5	162.5	4.2	51.0	1.5	18.0
µ-Slide I 0.8 Luer	2.5	200	4.5	50.0	1.5	12.0
µ-Slide I 0.8 Luer Glass	2.5	212.5	4.1	53.0	1.0	12.0
µ-Slide VI 0.4	0.6	30	4.1	47.0	5.0	58.0
µ-Slide VI 0.5 Glass	0.6	40	4.6	52.0	3.5	35.0
µ-Slide VI 0.1	0.17	1.7	0.3	3.3	19.0	245.0
µ-Slide ibiPore Membrane Flow	1.25	50	4.0	41.0	3.7	37.0
µ-Slide I Luer 3D	0.25 *	130 **	4.3	52.0	2.0	22.0
µ-Slide Spheroid Perfusion	0.005 *	45 **	0.3	2.7	–	–
µ-Slide y-shaped	2.8	110	3.8	42.0	6.0	67.0

* per well
** per channel

The indicated shear stress values for the µ-Slide y-shaped refer to the single channel area. Find details in Application Note 18: Shear Stress and Shear Rates in µ-Slide y-shaped (PDF).

	Perfusion Set yellow/green (#10964)		Inner diameter: 1.6 mm Total working volume: 13.3 ml Tube length: 50 cm		Dead volume of tubing: 1.0 ml Viscosity 0.0072 dyn s/cm²
	Growth area [cm²]	Channel volume [µl]	Flow rate [ml/min]		Shear stress [dyn/cm²]
			MIN	MAX	MIN	MAX
µ-Slide I 0.2 Luer	2.5	50	1.7	22.0	6.1	78.0
µ-Slide I 0.2 Luer Glass	2.5	62.5	1.8	26.0	4.2	60.0
µ-Slide I 0.4 Luer	2.5	100	2.8	34.0	2.6	31.0
µ-Slide I 0.4 Luer Glass	2.5	112.5	3.0	36.0	2.2	26.0
µ-Slide I 0.6 Luer	2.5	150	3.0	36.0	1.3	15.0
µ-Slide I 0.6 Luer Glass	2.5	162.5	3.0	36.0	1.1	13.0
µ-Slide I 0.8 Luer	2.5	200	3.0	36.0	0.8	8.5
µ-Slide I 0.8 Luer Glass	2.5	212.5	3.0	36.0	0.7	7.8
µ-Slide VI 0.4	0.6	30	3.0	35.0	3.7	43.0
µ-Slide VI 0.5 Glass	0.6	40	3.0	36.0	2.2	24.0
µ-Slide VI 0.1	0.17	1.7	0.2	3.0	16.5	224.0
µ-Slide ibiPore Membrane Flow	1.25	50	2.2	26.0	2.1	24.0
µ-Slide I Luer 3D	0.25 *	130 **	2.6	33.0	1.1	13.5
µ-Slide Spheroid Perfusion	0.005 *	45 **	0.3	2.6	–	–
µ-Slide y-shaped	2.8	110	2.6	32.0	4.2	50.0

* per well
** per channel

The indicated shear stress values for the µ-Slide y-shaped refer to the single channel area. Find details in Application Note 18: Shear Stress and Shear Rates in µ-Slide y-shaped (PDF).

	Perfusion Set orange (##10969)		Inner diameter: 1.6 mm Total working volume: 14.3 ml Tube length: 100 cm		Dead volume of tubing: 2.0 ml Viscosity 0.0072 dyn s/cm²
	Growth area [cm²]	Channel volume [µl]	Flow rate [ml/min]		Shear stress [dyn/cm²]
			MIN	MAX	MIN	MAX
µ-Slide I 0.2 Luer	2.5	50	1.6	17.0	5.8	60.0
µ-Slide I 0.2 Luer Glass	2.5	62.5	2.0	21.0	4.7	48.0
µ-Slide I 0.4 Luer	2.5	100	2.0	23.0	1.9	21.0
µ-Slide I 0.4 Luer Glass	2.5	112.5	2.0	26.0	1.5	19.0
µ-Slide I 0.6 Luer	2.5	150	2.2	26.0	1.0	10.0
µ-Slide I 0.6 Luer Glass	2.5	162.5	2.2	26.0	0.8	9.0
µ-Slide I 0.8 Luer	2.5	200	2.2	26.0	0.6	6.0
µ-Slide I 0.8 Luer Glass	2.5	212.5	2.2	26.0	0.5	5.0
µ-Slide VI 0.4	0.6	30	2.0	23.0	2.5	28.0
µ-Slide VI 0.5 Glass	0.6	40	2.0	23.0	1.5	16.0
µ-Slide VI 0.1	0.17	1.7	0.2	3.0	15.0	220.0
µ-Slide ibiPore Membrane Flow	1.25	50	2.0	23.0	1.9	21.0
µ-Slide I Luer 3D	0.25 *	130 **	2.0	24.0	0.9	10.0
µ-Slide Spheroid Perfusion	0.005 *	45 **	0.3	2.4	–	–
µ-Slide y-shaped	2.8	110	2.0	20.0	3.2	30.0

* per well
** per channel

The indicated shear stress values for the µ-Slide y-shaped refer to the single channel area. Find details in Application Note 18: Shear Stress and Shear Rates in µ-Slide y-shaped (PDF).

	Perfusion Set blue (#10961)		Inner diameter: 0.8 mm Total working volume: 12.4 ml Tube length: 15 cm		Dead volume of tubing: 0.075 ml Viscosity 0.0072 dyn s/cm²
	Growth area [cm²]	Channel volume [µl]	Flow rate [ml/min]		Shear stress [dyn/cm²]
			MIN	MAX	MIN	MAX
µ-Slide I 0.2 Luer	2.5	50	0.65	8.0	2.4	28.0
µ-Slide I 0.2 Luer Glass	2.5	62.5	0.76	10.0	1.9	23.0
µ-Slide I 0.4 Luer	2.5	100	0.76	10.0	0.7	9.0
µ-Slide I 0.4 Luer Glass	2.5	112.5	0.82	10.0	0.6	7.0
µ-Slide I 0.6 Luer	2.5	150	0.82	10.0	0.4	4.0
µ-Slide I 0.6 Luer Glass	2.5	162.5	0.82	10.0	0.3	3.5
µ-Slide I 0.8 Luer	2.5	200	0.82	10.0	0.2	2.4
µ-Slide I 0.8 Luer Glass	2.5	212.5	0.82	10.0	0.2	2.1
µ-Slide VI 0.4	0.6	30	0.82	10.0	1.0	12.0
µ-Slide VI 0.5 Glass	0.6	40	0.86	11.0	0.7	7.5
µ-Slide VI 0.1	0.17	1.7	0.20	2.8	15.0	205.0
µ-Slide ibiPore Membrane Flow	1.25	50	0.82	10.0	0.8	9.0
µ-Slide I Luer 3D	0.25 *	130 **	0.82	10.0	0.4	4.2
µ-Slide Spheroid Perfusion	0.005 *	45 **	0.25	2.5	–	–
µ-Slide y-shaped	2.8	110	0.80	10.0	1.3	15.5

* per well
** per channel

The indicated shear stress values for the µ-Slide y-shaped refer to the single channel area. Find details in Application Note 18: Shear Stress and Shear Rates in µ-Slide y-shaped (PDF).

	Perfusion Set white (#10963)		Inner diameter: 0.8 mm Total working volume: 12.6 ml Tube length: 50 cm		Dead volume of tubing: 0.25 ml Viscosity 0.0072 dyn s/cm²
	Growth area [cm²]	Channel volume [µl]	Flow rate [ml/min]		Shear stress [dyn/cm²]
			MIN	MAX	MIN	MAX
µ-Slide I 0.2 Luer	2.5	50	0.40	4.5	1.50	18.0
µ-Slide I 0.2 Luer Glass	2.5	62.5	0.40	4.5	1.00	10.0
µ-Slide I 0.4 Luer	2.5	100	0.40	5.0	0.40	4.5
µ-Slide I 0.4 Luer Glass	2.5	112.5	0.40	5.0	0.30	3.5
µ-Slide I 0.6 Luer	2.5	150	0.40	5.0	0.20	2.1
µ-Slide I 0.6 Luer Glass	2.5	162.5	0.40	5.0	0.15	1.8
µ-Slide I 0.8 Luer	2.5	200	0.40	5.0	0.10	1.2
µ-Slide I 0.8 Luer Glass	2.5	212.5	0.40	5.0	0.10	1.1
µ-Slide VI 0.4	0.6	30	0.40	5.0	0.50	6.1
µ-Slide VI 0.5 Glass	0.6	40	0.40	5.0	0.30	3.4
µ-Slide VI 0.1	0.17	1.7	0.14	2.6	10.50	190.0
µ-Slide ibiPore Membrane Flow	1.25	50	0.40	4.5	0.40	4.0
µ-Slide I Luer 3D	0.25 *	130 **	0.40	4.5	0.17	1.8
µ-Slide Spheroid Perfusion	0.005 *	45 **	0.25	2.5		–
µ-Slide y-shaped	2.8	110	0.40	5.0	0.70	7.5

* per well
** per channel

The indicated shear stress values for the µ-Slide y-shaped refer to the single channel area. Find details in Application Note 18: Shear Stress and Shear Rates in µ-Slide y-shaped (PDF).

	Perfusion Set grey (#10968)		Inner diameter: 0.8 mm Tube length: 100 cm Total working volume: 12.9 ml		Dead volume of tubing: 0.52 ml Viscosity 0.0072 dyn s/cm²
	Growth area [cm²]	Channel volume [µl]	Flow rate [ml/min]		Shear stress [dyn/cm²]
			MIN	MAX	MIN	MAX
µ-Slide I 0.2 Luer	2.5	50	0.2	2.9	0.75	10.40
µ-Slide I 0.2 Luer Glass	2.5	62.5	0.2	2.9	0.50	6.70
µ-Slide I 0.4 Luer	2.5	100	0.2	2.9	0.20	2.60
µ-Slide I 0.4 Luer Glass	2.5	112.5	0.2	2.9	0.15	2.10
µ-Slide I 0.6 Luer	2.5	150	0.2	2.9	0.08	1.20
µ-Slide I 0.6 Luer Glass	2.5	162.5	0.2	2.9	0.07	1.00
µ-Slide I 0.8 Luer	2.5	200	0.2	2.9	0.05	0.70
µ-Slide I 0.8 Luer Glass	2.5	212.5	0.2	2.9	0.04	0.60
µ-Slide VI 0.4	0.6	30	0.2	2.9	0.25	3.50
µ-Slide VI 0.5 Glass	0.6	40	0.2	2.9	0.15	2.00
µ-Slide VI 0.1	0.17	1.7	0.2	2.6	15.00	190.00
µ-Slide ibiPore Membrane Flow	1.25	50	0.2	2.9	0.18	2.60
µ-Slide I Luer 3D	0.25 *	130 **	0.2	2.9	0.08	1.20
µ-Slide Spheroid Perfusion	0.005 *	45 **	0.2	2.3	–	–
µ-Slide y-shaped	2.8	110	0.2	2.9	0.32	4.50

* per well
** per channel

The indicated shear stress values for the µ-Slide y-shaped refer to the single channel area. Find details in Application Note 18: Shear Stress and Shear Rates in µ-Slide y-shaped (PDF).

	Perfusion Set yellow (#10965)		Inner diameter: 0.5 mm Tube length: 15 cm Total working volume: 2.5 ml		Dead volume of tubing: 0.03 ml Viscosity 0.0072 dyn s/cm²
	Growth area [cm²]	Channel volume [µl]	Flow rate [ml/min]		Shear stress [dyn/cm²]
			MIN	MAX	MIN	MAX
µ-Slide I 0.2 Luer	2.5	50	0.16	2.20	0.60	7.90
µ-Slide I 0.2 Luer Glass	2.5	62.5	0.16	2.20	0.40	5.10
µ-Slide I 0.4 Luer	2.5	100	0.16	2.20	0.15	2.00
µ-Slide I 0.4 Luer Glass	2.5	112.5	0.16	2.20	0.12	1.60
µ-Slide I 0.6 Luer	2.5	150	0.16	2.20	0.07	0.90
µ-Slide I 0.6 Luer Glass	2.5	162.5	0.16	2.20	0.06	0.75
µ-Slide I 0.8 Luer	2.5	200	0.16	2.20	0.04	0.52
µ-Slide I 0.8 Luer Glass	2.5	212.5	0.16	2.20	0.03	0.45
µ-Slide VI 0.4	0.6	30	0.16	2.20	0.20	2.60
µ-Slide VI 0.5 Glass	0.6	40	0.16	2.20	0.12	1.50
µ-Slide VI 0.1	0.17	1.7	0.12	1.70	9.00	125.00
µ-Slide ibiPore Membrane Flow	1.25	50	0.16	2.20	0.15	2.00
µ-Slide I Luer 3D	0.25 *	130 **	0.16	2.20	0.07	0.93
µ-Slide Spheroid Perfusion	0.005 *	45 **	0.12	1.70	–	–
µ-Slide y-shaped	2.8	110	0.16	2.20	0.25	3.50

* per well
** per channel

The indicated shear stress values for the µ-Slide y-shaped refer to the single channel area. Find details in Application Note 18: Shear Stress and Shear Rates in µ-Slide y-shaped (PDF).

	Perfusion Set black (#10966)		Inner diameter: 0.5 mm Total working volume: 2.6 ml Tube length: 50 cm		Dead volume of tubing: 0.1 ml Viscosity 0.0072 dyn s/cm²
	Growth area [cm²]	Channel volume [µl]	Flow rate [ml/min]		Shear stress [dyn/cm²]
			MIN	MAX	MIN	MAX
µ-Slide I 0.2 Luer	2.5	50	0.08	1.1	0.30	3.90
µ-Slide I 0.2 Luer Glass	2.5	62.5	0.08	1.1	0.20	2.50
µ-Slide I 0.4 Luer	2.5	100	0.08	1.1	0.07	1.00
µ-Slide I 0.4 Luer Glass	2.5	112.5	0.08	1.1	0.06	0.80
µ-Slide I 0.6 Luer	2.5	150	0.08	1.1	0.03	0.45
µ-Slide I 0.6 Luer Glass	2.5	162.5	0.08	1.1	0.03	0.40
µ-Slide I 0.8 Luer	2.5	200	0.08	1.1	0.02	0.27
µ-Slide I 0.8 Luer Glass	2.5	212.5	0.08	1.1	0.02	0.24
µ-Slide VI 0.4	0.6	30	0.08	1.1	0.10	1.35
µ-Slide VI 0.5 Glass	0.6	40	0.08	1.1	0.06	0.70
µ-Slide VI 0.1	0.17	1.7	0.07	0.8	5.30	60.00
µ-Slide ibiPore Membrane Flow	1.25	50	0.08	1.1	0.07	1.00
µ-Slide I Luer 3D	0.25 *	130 **	0.08	1.1	0.03	0.45
µ-Slide Spheroid Perfusion	0.005 *	45 **	0.07	0.8	–	–
µ-Slide y-shaped	2.8	110	0.08	1.1	0.13	1.70

* per well
** per channel

The indicated shear stress values for the µ-Slide y-shaped refer to the single channel area. Find details in Application Note 18: Shear Stress and Shear Rates in µ-Slide y-shaped (PDF).

	Perfusion Set brown (#10967)		Inner diameter: 0.5 mm Total working volume: 2.7 ml Tube length: 100 cm		Dead volume of tubing: 0.2 ml Viscosity 0.0072 dyn s/cm²
	Growth area [cm²]	Channel volume [µl]	Flow rate [ml/min]		Shear stress [dyn/cm²]
			MIN	MAX	MIN	MAX
µ-Slide I 0.2 Luer	2.5	50	0.04	0.5	0.14	1.80
µ-Slide I 0.2 Luer Glass	2.5	62.5	0.04	0.5	0.09	1.15
µ-Slide I 0.4 Luer	2.5	100	0.04	0.5	0.04	0.45
µ-Slide I 0.4 Luer Glass	2.5	112.5	0.04	0.5	0.03	0.37
µ-Slide I 0.6 Luer	2.5	150	0.04	0.5	0.02	0.20
µ-Slide I 0.6 Luer Glass	2.5	162.5	0.04	0.5	0.01	0.18
µ-Slide I 0.8 Luer	2.5	200	0.04	0.5	0.01	0.12
µ-Slide I 0.8 Luer Glass	2.5	212.5	0.04	0.5	0.01	0.11
µ-Slide VI 0.4	0.6	30	0.04	0.5	0.05	0.60
µ-Slide VI 0.5 Glass	0.6	40	0.04	0.5	0.03	0.34
µ-Slide VI 0.1	0.17	1.7	0.04	0.5	3.00	37.00
µ-Slide ibiPore Membrane Flow	1.25	50	0.04	0.5	0.04	0.45
µ-Slide I Luer 3D	0.25 *	130 **	0.04	0.5	0.02	0.20
µ-Slide Spheroid Perfusion	0.005 *	45 **	0.04	0.5	–	–
µ-Slide y-shaped	2.8	110	0.04	0.5	0.06	0.80

* per well
** per channel