ibidi : Heating System, Universal Fit, for 1 Chamber

Heating System for live cell imaging applications including high-resolution and confocal microscopy

Stage top incubator that fits most inverted microscopes that have a regular frame or holder for multiwell plates
Excellent illumination of the sample – no condensation due to heated lid, optimal magnetic sample stabilization, and suitable for DIC
Perfectly suited for live cell imaging applications – full incubator conditions on the microscope
Available with or without Heated Lid

Complete system includes:

Applications

Live cell imaging that requires incubator conditions on the microscope, for example:
– Angiogenesis
– Cell migration, proliferation, wound healing
– Cell culture under flow
– Confocal microscopy
– Anaerobic conditions or oxidative stress

Rat fibroblasts (cell line)

Find Out More

Please find more detailed information about the planning, conduction, and data analysis of live cell imaging assays here.

Download the whole “Live Cell Imaging” Application Guide as a PDF here.

Specifications

Temperature Controller – Blue Line
Operating humidity	Max. 80% relative humidity (RH)
Environmental operating temperature	18–30°C/64–86°F (min 5°C/9°F less than set temperature)
Dimensions	90 mm x 170 mm x 230 mm
Weight	1720 g / 3.8 lbs
External power supply	AC 100–240 V, 50/60 Hz, 2 A

Heated Plate and Lid
Plate dimensions	127.5 x 85.5 x 12 mm³ (fits standard multiwell holders)
Lid dimensions	127.5 x 85.5 x 19 mm³
Height of the system	25.5 mm
Weight of incubation chamber with insert	330 g / 0.46 lbs
Gas inlet	Female Luer connector with Luer Lock
Restrictions for objective lenses
Optical:	None
Geometrical:	Possible collision with large objectives near the edges, narrowing the field of view
Custom openings	On request

Heating Stages for Live Cell Imaging – No Condensation

The independently controlled, heated glass lid of the ibidi Heating & Incubation System solves the problem of condensation in live cell imaging.

By heating the lid to a temperature higher than the plate, a vertical temperature gradient is created. This gradient and an active humidity control prevent the formation of condensation on the lid of the Petri dish. The temperature at the cells’ site is maintained at a constant 37 °C.

Stable and Homogeneous Temperature

Stable and consistent temperature distribution in the µ-Slide VI^0.4 (left) and the µ-Slide 8 Well (right) during incubation in the ibidi Heating System, Universal Fit. Images were acquired with a FLIR thermal camera.

Components of the ibidi Heating & Incubation System, Universal Fit – Blue Line

ibidi Temperature Controller – Blue Line

The Temperature Controller controls the temperature of the different heated components (Heated Lid and Heated Plate). Four control channels are available.

Technical Features:

Temperature stability: ± 0.2 °C
Temperature uniformity across the insert: ± 0.5 °C (Heating Insert for slides and dishes)
Temperature range: Room temperature – 45 °C
Control channels: 4
Includes IncuControl software instrument control and data recording via a notebook
External temperature sensor included
Adjustable alarm function included
Analog output for extreme noise reduction optional

Heated Plate for 1 Chamber

Can be used with inverted microscopy systems
Fits into both manual and automated stages
Can be placed into regular multiwell frames on your microscope
Adaptable to all types of microscopy chambers, such as µ-Slides and µ-Dishes plus non-ibidi formats

Heated Plate for 4 µ-Slides

Only included in the ibidi Heating System, Universal Fit, for 4 µ-Slides – Blue Line
Can be used with inverted microscopy systems
Ideal for use with motorized stages
Can be placed into regular multiwell frames on your microscope
Compatibe with all ibidi µ-Slides and non-ibidi slide formats

Heated Glass Lid, Universal Fit, for Use With CO₂ and Humidity

For use with CO₂, O₂, and humidity to provide a complete environmental chamber
Independently controlled glass lid, warmed to 40 °C, prevents the formation of condensed water on the lid of the microscopy chamber or plate
No scattering of light or shadow formation

Heating Inserts

For all ibidi µ-Slides
For µ-Dish^{35mm. high}
For µ-Dish^{35mm. low}
For LabTek^TM chambered coverglass
To be used with the Heated Plate for 1 chamber

Optional: Heating Insert Adapter for Perfusion Assays

To be used with the ibidi Pump System for live cell imaging under flow conditions

Gas Mixer for the Incubation Unit

The heated lid also comes with a gas inlet. The ibidi Gas Incubation System upgrades the ibidi Heating System to a complete stage top incubator for all live cell imaging applications, including high resolution and confocal microscopy.

The ibidi Gas Incubation System is suitable for various experimental conditions (e.g., pH or hypoxia). It is available in two versions: Gas Mixer for CO₂, Gas Mixer for CO₂ and O₂.

Live Cell Imaging Under Flow Conditions

The ibidi Heating & Incubation System can be adapted for live cell imaging under flow conditions. Connect the ibidi Pump System with the ibidi Heating System, Universal Fit, by using the Heating Insert Adapter for perfusion assays. This creates the perfect environment for long-term cell studies (several days) under flow conditions.

Overview of the Heating and Incubation System, Universal Fit – Blue Line

ibidi Heating System for Temperature Control of Multiwell Plates

ibidi has developed a Heating System for the use with K-Frame Stages or the Nikon Ti-S-E and Ti-S-ER Motorized Stage which hold multiwell plates. Thus, this system permits the user to control the temperature of samples held in multiwell plates.