ibidi : µ-Dish 35 mm, low Glass Bottom

จานเพาะเลี้ยงเซลล์ สำหรับการถ่ายภาพ ขนาด 35 มม. ชนิดขอบต่ำ ก้นทำจากแก้ว

A 35 mm imaging dish with a glass bottom and low walls for TIRF, single molecule and super-resolution microscopy

The microscopy dish contains a high-quality No. 1.5H Glass Coverslip bottom with very low thickness variability
Cell culture dish with low walls for a large access area during micromanipulation assays
Equipped with a tightly fitting lid that minimizes evaporation; suitable for DIC when using a DIC Lid
Surface Modification: #1.5H (170 µm +/- 5 µm) D 263 M Schott glass, sterilized
Pcs./Box: 60 (individually packed)

Applications

Cultivation and high-resolution microscopy of cells
Cell manipulation and microinjection using microcapillaries
Total Interference Reflection Fluorescence (TIRF) and single molecule applications
Super-Resolution Microscopy (STED, SIM, (F)PALM, (d)STORM) and Fluorescence Correlation Spectroscopy (FCS)
Immunofluorescence staining
Widefield and confocal fluorescence microscopy of living and fixed cells
Live cell imaging over extended time periods
Transfection assays
Differential interference contrast (DIC) when using a DIC Lid

Want to know if you should use a glass or a polymer bottom for your application? Find out here.

Specifications

Ø µ-Dish	35 mm
Volume	800 µl
Growth area	3.5 cm²
Coating area using 400 µl	4.1 cm²
Ø observation area	21 mm
Height with / without lid	9 / 7 mm
Bottom: Glass Coverslip No. 1.5H, selected quality, 170 µm +/- 5 µm

Fluorescence microscopy of fixed rat fibroblasts on a Glass Coverslip #1.5H. F-actin filaments are stained with phalloidin (green), nuclei are stained with DAPI (blue).

Technical Features

Standard format imaging dish with a 35 mm diameter for cell and tissue culture
Low walls for a large access area during micromanipulation experiments
Lid with locking feature for minimal evaporation
Bottom made from D 263 M Schott glass with a thickness of 170 µm +/- 5 µm
May require coating to promote cell attachment
Compatible with staining and fixation solvents
No autofluorescence
Fully biocompatible materials
Also available: µ-Dish^{35 mm, low} with a #1.5 ibidi Polymer Coverslip bottom with optimized adhesion, for diverse applications
Also available with high walls: µ-Dish^{35 mm, high} with an #1.5 ibidi Polymer Coverslip bottom for everyday use and µ-Dish^{35 mm, high} Glass Bottom for special microscopic applications

The Principle of the µ-Dish^{35 mm, low} Glass Bottom

Low Walls for Easy Micromanipulation of Cells

The ibidi μ-Dishes with low walls (i.e., μ-Dish^{35 mm, low} and µ-Dish^{35 mm, low} Glass Bottom) are perfectly suited for micromanipulation assays, such as intracellular injections, micropipette chemotaxis assays, or tissue treatment and microscopy. Due to the low walls of these µ-Dishes, instrumentation (e.g., electrodes) can be positioned exactly using a micromanipulator. The excellent optical quality makes the ibidi µ-Dishes ideally suited for single-cell handling and high-resolution live cell imaging. For all standard cell culture applications, we also offer the µ-Dish^{35 mm, high} product family with high walls.

The Coverslip Bottom

The µ-Dish^{35 mm, low} Glass Bottom comes with a thin #1.5H Glass Coverslip Bottom made from D 263 M Schott borosilicate glass that has the highest optical quality. ibidi developed these glass surfaces specifically for TIRF, super-resolution microscopy, and single molecule microscopy. The dish is also available with an #1.5 ibidi Polymer Coverslip Bottom for optimal cell adhesion and high-resolution microscopy in a variety of assays.

Learn more about the coverslip bottom or the different surfaces of the ibidi chambers here.

Lid with Locking Feature for Minimized Evaporation

All ibidi µ-Dishes are equipped with the special lid-locking feature. The locking position minimizes evaporation, and thereby provides excellent conditions for long-term studies in a non-humidified environment. Gas exchange (carbon dioxide or oxygen) during cell culture is maintained thanks to the gas-permeable plastic material of the dish.

TIP: Use the locking feature if minimal evaporation is required (e.g., outside incubators, non-humidified microscopy stages, etc.).

Experimental Example

Microinjection of cells using the µ-Dish^{35 mm, low} Glass Bottom. The microcapillary (e.g., Eppendorf Femtotips^®) is connected to a micromanipulator, which is placed directly on the stage of the confocal fluorescence microscope. The low walls of the µ-Dish allow the capillary to easily access the cells.

Comparison of the ibidi Dishes^{35 mm, low}

Please find a detailed comparison of the material specifications, including suitable microscopy applications for the ibidi Polymer Coverslip, the ibidi Glass Bottom, and further materials, here.

The ibidi µ-Dishes: High Walls vs. Low Walls

µ-Dish^{35 mm, high} with high walls for all standard cell culture applications

µ-Dish^{35 mm, low} with low walls provides a larger access area useful for micromanipulation

	µ-Dish^{35 mm, low}	µ-Dish^{35 mm, low} Glass Bottom
Bottom material	#1.5 ibidi Polymer Coverslip	#1.5H ibidi Glass Coverslip
Bottom thickness	180 µm (+10/-5 µm)	170 µm (+/-5 µm)
Bottom: gas permeability	Yes	No
Available surfaces	ibiTreat (tissue culture-treated), Uncoated (hydrophobic)	Uncoated glass
Lid	Lid with locking feature	Lid with locking feature
Packaging	Sterile, individually packed	Sterile, individually packed
Quantity	60 pcs./box	60 pcs./box
High wall version available?	Yes: µ-Dish^{35 mm, high} for all standard cell culture applications	Yes: µ-Dish^{35 mm, high} Glass Bottom (#1.5H) with highest optical quality and the cost-effective Glass Bottom Dish 35 mm (#1.5)
Gridded version available?	Yes: µ-Dish^{35 mm, low} Grid-500	No