Channel slides with a #1.5H glass bottom for flow applications; different heights and volumes available

Applications

Want to know if you should use a glass or a polymer bottom for your application? Find out here.

Specifications

Outer dimensions25.5 x 75.5 mm² (w x l)
Channel length50 mm
Channel width5 mm
AdaptersFemale Luer
Volume per reservoir60 μl
Growth area2.5 cm2
Bottom: Glass coverslip No. 1.5H, selected quality, 170 µm +/- 5 µm

Because of the adhesive layer, the channel height of the µ-Slide I Luer Glass Bottom increases by 50 µm compared to the µ-Slide I Luer.

µ-Slide I Luer Glass Bottom0.20.40.60.8
Channel height250 µm450 µm650 µm850 µm
Channel volume62.5 µl112.5 µl162.5 µl212.5 µl
Coating area5.2 cm²5.4 cm²5.6 cm²5.8 cm²

Find Out More

Please find more detailed information about the planning, conduction, and data analysis of cell culture under flow assays here.

Download the whole “Cell Culture Under Flow” Application Guide as a PDF here.

Technical Features

The Principle of the µ-Slide I Luer Glass Bottom

The Glass Bottom

The µ-Slide I Luer Glass Bottom comes with a thin #1.5H Glass Coverslip Bottom that has the highest optical quality and is ideally suitable for high-resolution microscopy, TIRF, and super-resolution microscopy. To promote cell attachment, a surface coating might be required prior to cell seeding.

Find more information and technical details about the coverslip bottom of the ibidi chambers here.

Choosing the Right Channel Height for Flow Applications

Which Experiment Are You Planning?

Low channels are more suitable for flow applications.
High channels are more suitable for static cell culture.

For flow assays with small amounts of medium and high values of shear stress0.2 channel
For a wide range of shear stress0.4 channel
For controlling low values of shear stress < 2 dyne/cm20.6 and 0.8 channels

Select the ideal Perfusion Set for your flow application here.

Cross Section of the Different Channel Heights

* not recommended for static culture over more than 6 hours

Experimental Example

Cells Cultured Under Static or Flow Conditions

Static cultivation

Flow cultivation