ibidi : µ-Slide 4 Well Glass Bottom

แผ่นปิดแบบแชมเบอร์มี 4 หลุม #1.5H glass bottom เหมาะสำหรับใช้ใน TIRF และการใช้งานโมเลกุลเดี่ยว

การใช้งาน

A chambered coverslip with 4 wells and a #1.5H glass bottom, suitable for use in TIRF and single molecule applications

Applications

Specifications

Outer dimensions (w x l)25.5 x 75.5 mm²
Number of wells4
Dimensions of wells
(w x l x h)
21.6 x 11.4 x 9.3 mm³
Volume per well700 µl
Total height with / without lid10.8 / 9.5 mm
Growth area per well2.5 cm²
Coating area per well4.1 cm²
Bottom: Glass coverslip No. 1.5H, selected quality, 170 µm +/- 5 µm

Technical Features

Experimental Example

Surface-near F-actin network of a Dictyostelium discoideum DdLimE-GFP cell. Live cell imaging on a Glass Coverslip #1.5H using Total Interference Reflection Fluorescence (TIRF) microscopy.

ibidi Polymer Coverslip vs. ibidi Glass Bottom

ibidi Polymer Coverslipibidi Glass Bottom
Optical properties
Refractive index (nD 589 nm)1.521.52
Abbe number5655
Thickness#1.5 (180 µm)#1.5H (170 µm)
MaterialMicroscopy plasticD 263M Schott borosilicate glass
AutofluorescenceLowLow
TransmissionVery high (even ultraviolet)High (ultraviolet restrictions)
Birefringence (DIC)Low (DIC compatible*)Low (DIC compatible*)
Other aspects
Surface modificationsibiTreat – tissue culture treated
Uncoated – hydrophobic
Only glass
Protein coatingsCompatibleCompatible
Gas permeableYesNo
Material flexibilityHighLow
BreakableNoYes
ApplicationsFluorescence microscopyTIRF and single photon