ฝาปิดพิเศษสำหรับการใช้เทคนิคแสงแบบ Differential Interference Contrast (DIC)
- ใช้กับกล้องจุลทรรศน์ที่ใช้เทคนิคแสงแบบ Differential Interference Contrast (DIC)
- สามารถใช้กับจานเพาะเลี้ยงเซลล์ของ ibidi แบบ 35 มม. ทุกแบบ
- สามารถใช้แอลกอฮอล์ทำความสะอาดเพื่อนำกลับมาใช้ซ้ำได้
Special lid with a polymer with low birefringence for use in Differential Interference Contrast (DIC) microscopy
- Ideal for use in Differential Interference Contrast (DIC) microscopy
- Suitable for use with all ibidi 35 mm µ-Dishes
- Can be alcohol-sterilized and then be reused
- Made from special polymer with low birefringence
- Pcs./Box: 15 (individually packed)
Applications
- Differential Interference Contrast (DIC) microscopy
- Sterile cell culture
- Live cell imaging
Specifications
Compatible with | ibidi µ-Dishes 35 mm, high ibidi µ-Dishes 35 mm, low |
Material | Polymer with low birefringence |
Temperature stability | Up to 80°C/175°F (not autoclavable) |
Technical Features
- Lid with low birefringence for excellent DIC imaging
- Compatible with all ibidi 35 mm µ-Dishes
- Not compatible with the Glass Bottom Dish 35 mm
- Individually packed and sterilized
The DIC Beam Path
For excellent imaging with a differential interference contrast (DIC) microscope, all elements in the optical pathway need to be DIC-compatible. They must exhibit a low birefringence, meaning that there is only low interference with the polarized light. Just one single incompatible element in the beam path of the transmitted light would destroy the orientation of the polarized light, leading to disturbance of the DIC imaging effect. When using the ibidi µ-Dishes combined with the special DIC Lids for µ-Dishes, excellent DIC images can be generated.
DIC Compatibility of Different Materials
Compatible |
DIC Lid for µ-Slides | DIC Lid for µ-DishesOpen µ-Slides | µ-Dishes | µ-Plates without lidGlass coverslips, glass slidesibidi Polymer Coverslipibidi Glass CoverslipAll ibidi Heating SystemsWater, cell culture medium |
Not compatible |
Lids of all µ-Slides, µ-Dishes and µ-PlatesChannel µ-Slides and Ph+ µ-SlidesCell culture flasks, Petri dishes |
Experimental Example
Human keratinocytes on an ibiTreat surface. Imaging with a DIC objective lens 10x.