จานเพาะเลี้ยงเซลล์พร้อมซิลิโคนแบบ 4 ช่อง – สามารถกำหนดช่องว่างระหว่างเซลล์ได้ 4 ส่วน สำหรับการทดลองแบบ wound healing, การทำ cell migration, การทำ 2D invasion หรือการเลี้ยงเซลล์
- เหมาะสำหรับการทดลองแบบ wound healing
- สามารถทำการทดลองซ้ำได้เนื่องจากช่องว่างของส่วนที่เป็นซิลิโคนมีขนาด 500 µm และตรงกลางมีขนาด 1000 µm โดยซิลิโคนติดแน่นกับก้นจานมั่นใจได้ว่าไม่มีการรั่วซึมของอาหารเลี้ยงเซลล์
- สำหรับการทดลอง 4 experiments โดยมีช่องว่างตรงกลางเพื่อดูการ migration ของเซลล์ทั้งหมด
- มีเทคโนโลยี ibiTreat surface เพื่อการเจริญเติบโตของเซลล์ในอุดมคติ
A 4 well silicone insert with 4 defined cell-free gaps, suitable for wound healing, migration assays, 2D invasion assays, and co-cultivation of cells
- Complete solution for wound healing experiments, requiring only a few steps from sample preparation to image analysis
- Reproducible experiments owing to: four defined 500 µm cell free gaps and one 1000 µm center gap, no leakage during cultivation, and no material being left behind after the insert’s removal
- Suitable for 4 experiments, including a center gap that allows to observe the migration of all cell types
- Individual inserts for single experiments
- Ideal cell growth on ibiTreat surface
- Surface Modification: ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized
Pcs./Box: 30 (individually packed)
Product Variation: in µ-Dish 35 mm, high
Applications
Specifications
| Number of wells | 4 |
| Outer dimensions | Ø 17 mm |
| Volume per well | 110 µl |
| Growth area per well | 0.35 cm² |
| Coating area per well | 1.23 cm² |
| Width of cell free gap | |
| – Two cell fronts | 500 µm +/- 100 µm |
| – Four cell fronts (center) | 1000 µm +/- 100 µm |
| Material | Biocompatible silicone |
| Bottom | No bottom – sticky underside |
Technical Features
- Culture-Insert 4 Well, pre-inserted into the µ-Dish 35 mm, high
- Biocompatible silicone material with adhesive underside
- Ready-to-use: the Culture-Insert is already placed in the µ-Dish 35 mm, high
- Clean surface after insert removal without remaining material
- Find full specifications and technical details of the µ-Dish here
Principle for Wound Healing and Migration Assays
Various Applications
Wound Healing / Migration
The Culture-Insert 4 Well allows for wound healing and migration assays with four cell-free gaps with a 0.5 mm gap width, and one center area with a 1 mm diagonal distance. Compared to the 2 well version, more cells can be analyzed and a higher throughput is realized. Two cell-free gaps create a technical replicate in the very same well.
Two Conditions of Wound Healing / Migration
With the Culture-Insert 4 Well, a wound healing experiment and a control experiment can be done in the same well. The control cells can be pre-treated with any kind of treatment (e.g., drugs, plasmids, mRNA, or virus particles).
Four Conditions for Co-Cultivation / Invasion
By seeding up to four different cell types, the invasiveness of adherent cells can be analyzed. Non-invasive cell types can be cultured next to each other, sharing the same medium. This co-culture allows the cells to communicate and share factors for differentiation studies or long-term culturing.
Invasion and co-cultivated cells can be imaged by using high-resolution fluorescence microscopy.
Visualization of Cell Migration
Live cell imaging of a wound healing and migration assay using the MCF7 cell line. Objective lens 10x, phase contrast microscopy.
