ibidi : Culture-Insert 2 Well in µ-Dish 35 mm

ibidi - 81176, 80206

Culture-Insert 2 Well in µ-Dish 35 mm - จานเพาะเลี้ยงเซลล์ขนาด 35 มม. สำหรับ wound healing assay



จานเพาะเลี้ยงเซลล์พร้อมซิลิโคนแบบ 2 ช่อง – สามารถกำหนดช่องว่างระหว่างเซลล์ สำหรับการทดลองแบบ wound healing, การทำ cell migration, การทำ 2D invasion หรือการเลี้ยงเซลล์

  • เหมาะสำหรับการทดลองแบบ wound healing 
  • สามารถทำการทดลองซ้ำได้เนื่องจากช่องว่างของส่วนที่เป็นซิลิโคนมีขนาด 500 µm เท่ากันทุกชิ้น โดยซิลิโคนติดแน่นกับก้นจานมั่นใจได้ว่าไม่มีการรั่วซึมของอาหารเลี้ยงเซลล์
  • สำหรับการทดลอง 1 experiments
  • มีเทคโนโลยี ibiTreat surface เพื่อการเจริญเติบโตของเซลล์ในอุดมคติ

A 2 well silicone insert with a defined cell-free gap, suitable for wound healingmigration assays2D invasion assays, and co-cultivation of cells

  • Complete solution for wound healing experiments, requiring only a few steps from sample preparation to image analysis
  • Reproducible experiments owing to: a defined 500 µm cell free gap, no leakage during cultivation, and no material being left behind after the insert’s removal
  • Individual inserts for single experiments
  • Ideal cell growth on ibiTreat surface
  • Surface Modification: ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized
    Product Variation: in µ-Dish 35 mm, low
    Pcs./Box: 30
  •  Surface Modification: ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized
    Product Variation: in µ-Dish 35 mm, high
    Pcs./Box: 30



Number of wells2
Outer dimensions8.4 x 8.4 x 5 mm (w x l x h)
Volume per well70 µl
Growth area per well0.22 cm²
Coating area per well0.82 cm²
Width of cell free gap500 µm +/- 100 µm
MaterialBiocompatible silicone
BottomNo bottom – sticky underside

Technical Features

Culture-Insert 2 Well in µ-Dish 35 mm, high

Culture-Insert 2 Well in µ-Dish 35 mm, low

Principle for Wound Healing and Migration Assays

xperimental Examples

Visualization of Cell Migration in Wound Healing Assays

Wound healing and migration assays are done by seeding cells into the Culture-Insert 2 Well. After cell attachment, a cell-free gap is created in which the cell migration can be visualized.

Live cell imaging of a wound healing and migration assay using the MCF7 cell line. Objective lens 10x, phase contrast microscopy.

Cell Invasion of Co-Culture Assays

The Culture-Insert 2 Well can also be used for seeding two different cell types. After the removal of the insert, the cell fronts can be analyzed for their invasional behavior.

Endothelial cells (left) and trophoblast cells (right) were cultured in an ibidi µ-Dish 35 mm, high with a Culture-Insert 2 Well. Cells were allowed to migrate towards each other after removal of the insert prior to fixation, staining, and imaging. Cells were stained with an antibody against VE-cadherin (magenta), phalloidin for F-actin (cyan), and Cytokeratin 8 (yellow). Image used with permission from Derek Sung.

Culture-Inserts vs. Scratch Assay

At first glance, the methods of scratching and placing a Culture-Insert seem to be two very similar approaches to create a cell-free gap. However, at a closer look, these two methods differ in important aspects that could influence the outcome of the assay:

ibidi Culture-InsertsScratch assay
Gap creationCell seeding into designated areasScratching the cell surface with a needle or pipet tip
Gap sizeDefinedVarying (i.e., not reproducible)
Gap surface residuesNoExtracellular matrix residues possible
Vessel surface damageNoYes, due to mechanical scratching on the surface
Cell damageNoYes, due to scratching the cells
Segregation & signaling
of necrotic/apoptotic cells
Internal reference *YesNo