Chamber slide แบบ 2 ช่อง – ประกอบด้วย intermediate plate สำหรับเทคนิคแสงแบบ Phase contrast และการถ่ายภาพความละเอียดสูง
- เหมาะสำหรับ Phase contrast โดยไม่ทำให้เกิด meniscus
- แบบ All-in-one chamber slide สำหรับการเพาะเลี้ยงเซลล์ และการถ่ายภาพด้วยกล้องจุลทรรศน์
- ก้นภาชนะมีความบางเหมือนกับ Coverslip สามารถใช้ในการถ่ายภาพความละเอียดสูงได้
- สามารถทำ immunofluorescence ได้รวดเร็วและง่าย โดยไม่มี coverslips และไม่มีการรั่วซึม
A chambered coverslip with 2 wells and a special intermediate plate for excellent phase contrast and high-end fluorescence microscopy
- Excellent phase contrast over the entire well – no meniscus
- All-in-one chamber slide used for cell culture and microscopy
- Observation of the sample through the coverslip-like bottom using high resolution microscopy
- Fast and easy immunofluorescence, with no coverslips and no leakage
- Surface Modification: ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized Pcs./Box: 15 (individually packed)
Applications
- Cultivation and microscopy of cell cultures
- Transfection assays
- Fluorescence microscopy of both living and fixed cells
- Live cell imaging over extended time periods
Specifications
| Outer dimensions (w x l) | 25.5 x 75.5 mm² |
| Number of wells | 2 |
| Dimensions of wells (w x l x h) | 21.6 x 23.8 x 9.3 mm³ |
| Volume per well | 1500 µl |
| Liquid height | 3.0 mm |
| Total height with / without lid | 10.8 / 9.5 mm |
| Growth area per well | 5.1 cm² |
| Coating area per well | 11.4 cm² |
| Bottom | ibidi Polymer Coverslip |
Technical Features
- Open µ-Slide with 2 independent wells
- Intermediate plate in each well to avoid meniscus formation
- Easy filling, without air bubbles, through the lateral slot
- Excellent optical quality for high end microscopy
- Ph+ feature for perfect phase contrast—no meniscus formation
- Compatible with staining and fixation
- ibiTreat surface for optimal cell adhesion
- Made of biocompatible plastic material – no glue and no leaking
- Also available with a glass bottom
Meniscus-Free Phase Contrast Microscopy
µ-Slide 2 Well | µ-Slide 2 Well Ph+ |
Top View |  |
The µ-Slide 2 Well Ph+ (Phase Contrast +) is designed for excellent optical quality, especially for cell culture when normal phase contrast microscopy is being used. Opposed to the classic µ-Slide 2 Well, the Ph+ version provides a special intermediate plate in the center of the well. This plate flattens the meniscus that disturbs the phase contrast effect in normal open wells. Openings near the corners provide access to the wells for the easy filling and aspirating of liquids.
This innovative technique supports meniscus-free phase contrast microscopy in a very convenient manner.
Working with the µ-Slide 2 Well Ph+ Diminishes the Meniscus and Increases the Area of Well Contrasted Cells
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The illustration on the left shows the perturbing effect of a meniscus. Light is refracted on the air-water-interface, leading to poor contrast in microscopy. Only the small center part exhibits satisfying phase contrast.
Working with the µ-Slide 2 Well Ph+ diminishes the meniscus and increases the area of nicely contrasted cells. This nice contrast is due to the parallel beam path that is created by the plate.
Comparison Ph+ Well versus Standard Well: No Meniscus Effect in Ph+ Well
Standard Well
Poor Phase Contrast
Excellent Fluorescence Microscopy
µ-Slide 2 Well Ph+
Excellent Phase Contrast
Excellent Fluorescence Microscopy
