ibidi : µ-Dish 35 mm, low Grid-500

จานเพาะเลี้ยงเซลล์แบบก้นบางพอลิเมอร์พิเศษ ขนาด 35 มม มี Grid ขนาด 500 µm (ขอบต่ำ) – เหมาะสำหรับการถ่ายภาพ และหาตำแหน่งของเซลล์

เหมาะสำหรับการหาตำแหน่งเซลล์หรือกลุ่มเซลล์
ก้นภาชนะทำมาจากพอลิเมอร์พิเศษของ ibidi มีความบางเท่ากับ coverslip

มีเทคโนโลยี ibiTreat surface เพื่อการเจริญเติบโตของเซลล์ในอุดมคติ
มีฝาปิด พร้อมตำแหน่งล็อคที่ขอบจาน ช่วยลดการระเหย
เหมาะสำหรับเทคนิค DIC เมื่อใช้ร่วมกับฝาปิดพิเศษสำหรับเทคนิค DIC

A 35 mm imaging dish with an ibidi Polymer Coverslip bottom, low walls, and an imprinted 500 µm cell location grid

Ideal for locating cells or cell clusters
Unique ibidi Polymer Coverslip combining
- Excellent cell culture conditions; and
- Supreme optical quality for high resolution microscopy
Ideal cell growth conditions provided by the ibiTreat surface
Lid with lock position, which minimizes evaporation
Suitable for DIC, when used with the special DIC Lid
Surface Modification: ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized
Pcs./Box: 60 (individually packed)
Grid: Grid-500

Applications

Relocating cells or cell clusters, e.g., transfected cells for clone picking
Counting events per defined area (e.g., for calculating transfection efficiency)
Providing a reference structure for cell movements
Treatment of distinct single cells, such as in microinjection
Following axon growth on a defined scale bar

Want to know if you should use a glass or a polymer bottom for your application? Find out here.

Specifications

Grid repeat distance	500 µm (+/- 1 %)
Ø µ-Dish	35 mm
Volume	0.8 ml
Growth area	3.5 cm²
Coating area using 400 µl	4.1 cm²
Ø observation area	21 mm
Height with lid	9 mm
Bottom: ibidi Polymer Coverslip

Technical Features

Standard format dish with a 35 mm diameter
Grid with a 500 µm repeat distance
Lettered and numbered fields (A-U; 1-20)
Also available with a glass bottom: µ-Slide 35 mm, high glass bottom with Grid-50 or with Grid-500
Ideal cell growth conditions provided by the ibiTreat tissue culture treated surface
Made of biocompatible material using no glues, consequently no cells are harmed
Lid with lock position, which minimizes evaporation
Grid-500 also available in a µ-Slide 8 Well version

Layout Grid-500

Lid with Locking Feature for Minimized Evaporation

All ibidi µ-Dishes are equipped with the special lid locking feature. The locking position minimizes evaporation and thereby provides excellent conditions for long-term studies in a non-humidified environment. Gas exchange (carbon dioxide, or oxygen) during cell culture is maintained thanks to the gas-permeable plastic material of the dish.

TIP: Use the locking feature only if minimal evaporation is required (e.g., outside incubators, non-humidified microscopy stages, etc.).

Experimental Examples

Fluorescence microscopy image of murine cerebellar Purkinje neurons cultured in an ibidi µ-Dish^{35 mm, low} Grid-500. The cells were stained against the IP3 receptor (magenta) and imaged using a Zeiss LSM800 confocal microscope with a 40x objective lens. Image by Masahiko Tanaka, Atsushi Nakano, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, Japan.

ibidi µ-Dish Family: Versions and Variations

µ-Dish^{35 mm, high}

High walls:
Used for all standard applications

µ-Dish^{35 mm, low}

Low walls:
Provide greater cell access (useful for micromanipulation)

ibidi Polymer Coverslip vs. ibidi Glass Bottom

	ibidi Polymer Coverslip	ibidi Glass Bottom
Optical properties
Refractive index (n_D 589 nm)	1.52	1.52
Abbe number	56	55
Thickness	#1.5 (180 µm)	#1.5H (170 µm)
Material	Microscopy plastic	D 263M Schott borosilicate glass
Autofluorescence	Low	Low
Transmission	Very high (even ultraviolet)	High (ultraviolet restrictions)
Birefringence (DIC)	Low (DIC compatible*)	Low (DIC compatible*)
Other aspects
Surface modifications	ibiTreat – tissue culture treated Uncoated – hydrophobic	Only glass
Protein coatings	Compatible	Compatible
Gas permeable	Yes	No
Material flexibility	High	Low
Breakable	No	Yes
Applications	Fluorescence microscopy	TIRF and single photon