จานเพาะเลี้ยงเซลล์สำหรับการถ่ายภาพ ขนาด 35 มม. (ขอบต่ำ) – เหมาะสำหรับการถ่ายภาพด้วยกล้องจุลทรรศน์ และ micromanipulator

• เหมาะสำหรับ micromanipulator
• ก้นภาชนะทำมาจากพอลิเมอร์พิเศษของ ibidi มีความบางเท่ากับ coverslip

• มีเทคโนโลยี ibiTreat surface เพื่อการเจริญเติบโตของเซลล์ในอุดมคติ
• มีฝาปิด พร้อมตำแหน่งล็อคที่ขอบจาน ช่วยลดการระเหย
• เหมาะสำหรับเทคนิค DIC เมื่อใช้ร่วมกับฝาปิดพิเศษสำหรับเทคนิค DIC
• มี grid สำหรับดูตำแหน่งของเซลล์ และการนับ

A 35 mm imaging dish with a polymer coverslip bottom and low walls for high-end microscopy and micromanipulation

• Cell culture dish with low walls for a large access area during micromanipulation assays
• Cells are imaged on a microscopy dish with a No. 1.5 ibidi Polymer Coverslip Bottom that has the highest optical quality and provides optimal cell adherence
• Lid equipped with locking feature that minimizes evaporation
• Surface Modification: Uncoated: #1.5 polymer coverslip, hydrophobic, sterilized
  Pcs./Box: 60 (individually packed)
• Surface Modification: ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized
  Pcs./Box: 60 (individually packed)

Applications

• Cell manipulation, micromanipulation, and microinjection (e.g., using a micromanipulation pipette, a glass capillary, or a micromanipulator)
• Cultivation and high-resolution microscopy of cells
• Immunofluorescence staining
• Widefield and confocal fluorescence microscopy of living and fixed cells
• Live cell imaging over extended time periods
• Transfection assays
• Differential interference contrast (DIC) when using a DIC Lid
• Cell location and counting when using the gridded version

Want to know if you should use a glass or a polymer bottom for your application? Find out here.

Specifications

Ø µ-Dish	35 mm
Volume	800 µl
Growth area	3.5 cm2
Coating area using 400 µl	4.1 cm2
Ø observation area	21 mm
Height with / without lid	9 / 7 mm
Bottom: ibidi Polymer Coverslip

Technical Features

• Standard format imaging dish with a 35 mm diameter for tissue culture
• Low walls for a large access area during the micromanipulation of cells; also available with high walls with a standard height of 12 mm for easy handling: µ-Dish ^{35 mm, high}
• ibiTreat (tissue culture-treated) surface for optimal cell adhesion and cell growth
• Lid with locking feature for minimal evaporation
• No autofluorescence
• Biocompatible polymer material—no glue, no leaking
• Also available as µ-Dish ^{35 mm, low} Glass Bottom with a #1.5H Glass Coverslip Bottom, which is specifically suited for TIRF and single-molecule applications
• Available with a 500 µm grid: µ-Dish ^{35 mm, low} Grid-500

The Principle of the µ-Dish ^{35 mm, low}

Low Walls for Easy Micromanipulation of Cells

The ibidi μ-Dishes with low walls (i.e., μ-Dish ^{35 mm, low} and µ-Dish ^{35 mm, low} Glass Bottom) are perfectly suited for micromanipulation assays, such as intracellular injections, micropipette chemotaxis assays, or tissue treatment and microscopy. Due to the low walls of these µ-Dishes, instrumentation (e.g., electrodes) can be positioned exactly using a micromanipulator. The excellent optical quality makes the ibidi µ-Dishes ideally suited for single-cell handling and high-resolution live cell imaging. For all standard cell culture applications, we also offer the µ-Dish ^{35 mm, high} product family with high walls.

The Coverslip Bottom

The µ-Dish ^{35 mm, low} comes with a thin ibidi Polymer Coverslip Bottom that has the highest optical quality (comparable to glass) and is ideally suitable for high-resolution microscopy. It is also available as µ-Dish ^{35 mm, low} Glass Bottom with a #1.5H Glass Coverslip Bottom for TIRF or single molecule microscopy.

The ibiTreat Surface

ibiTreat (tissue culture-treated) is our most recommended surface modification, because almost all adherent cells grow well on it without the need for any additional coating.

Learn more about the coverslip bottom or the different surfaces of the ibidi chambers here.

Lid with Locking Feature for Minimized Evaporation

All ibidi µ-Dishes are equipped with a special lid-locking feature. The locking position minimizes evaporation and thereby provides excellent conditions for long-term studies in a non-humidified environment. Gas exchange (carbon dioxide or oxygen) during cell culture is maintained thanks to the gas-permeable polymer material of the dish.

TIP: Use the locking feature if minimal evaporation is required (e.g., outside incubators, non-humidified microscopy stages, etc.).

Experimental Examples

Microinjection of cells using a µ-Dish ^{35 mm, low}. A glass microinjection capillary is connected to a micromanipulator directly on the stage of a confocal fluorescence microscope. The low walls of the µ-Dish 35 mm, low allow for easy access of the cells with the capillary.

Live cell imaging of Dictyostelium discoideum (DdLimE-GFP mutant) in the µ-Dish ^{35 mm, low}. The image is an overlay of brightfield and fluorescence microscopy showing the recruitment of the LIM domain-containing protein E towards the pseudopodia. Objective lens 20x/0.5, Zeiss LSM 510, scale bar = 20 µm.

Comparison of the ibidi Dishes ^{35 mm, low}

Please find a detailed comparison of the material specifications, including suitable microscopy applications for the ibidi Polymer Coverslip, the ibidi Glass Bottom, and further materials, here.

	µ-Dish 35 mm, low	µ-Dish 35 mm, low Glass Bottom
Bottom material	#1.5 ibidi Polymer Coverslip	#1.5H ibidi Glass Coverslip
Bottom thickness	180 µm (+10/-5 µm)	170 µm (+/-5 µm)
Bottom: gas permeability	Yes	No
Available surfaces	ibiTreat (tissue culture-treated), Uncoated (hydrophobic)	Uncoated glass
Lid	Lid with locking feature	Lid with locking feature
Packaging	Sterile, individually packed	Sterile, individually packed
Quantity	60 pcs./box	60 pcs./box
High wall version available?	Yes: µ-Dish 35 mm, high for all standard cell culture applications	Yes: µ-Dish 35 mm, high Glass Bottom (#1.5H) with highest optical quality and the cost-effective Glass Bottom Dish 35 mm (#1.5)
Gridded version available?	Yes: µ-Dish 35 mm, low Grid-500	No

The ibidi µ-Dishes: High Walls vs. Low Walls

µ-Dish ^{35 mm, high} with high walls for all standard cell culture applications

µ-Dish ^{35 mm, low} with low walls provides a larger access area useful for micromanipulation