จานเพาะเลี้ยงเซลล์แบบก้นแก้ว สำหรับการถ่ายภาพ ขนาด 35 มม. แบบมี grid ขนาด 500 ไมครอน เพื่อการหาตำแหน่งเซลล์
- ออกแบบมาสำหรับการหาตำแหน่งเซลล์
- ง่ายต่อการนับเซลล์ต่อพื้นที่
- ก้นภาชนะทำมาจากแก้วเหมาะสำหรับกล้องจุลทรรศน์แบบ TIRF
A 35 mm imaging dish with a glass bottom and an imprinted 500 µm cell location grid
- Ideal for locating cells or cell clusters
- Easy cell counting per defined area
- Glass coverslip for TIRF and single molecule applications
- Surface Modification: #1.5H (170 µm +/- 5 µm) D 263 M Schott glass, sterilized
Pcs./Box: 30 (individually packed)
Grid: Grid-500
Applications
- Locating cells or cell clusters, e.g., transfected cells for clone picking
- Counting events per defined area (e.g., when calculating transfection efficiency)
- Providing a reference structure for cell movements
- Treatment of distinct single cells, such as in microinjection
- Following axon growth on a defined scale bar
Want to know if you should use a glass or a polymer bottom for your application? Find out here.
Specifications
Geometry of the µ–Dish 35 mm, high Glass Bottom
| Diameter dish | 35 mm |
| Volume | 2 ml |
| VGrowth area | 3.5 cm2 |
| Coating area using 400 µl | 4.1 cm2 |
| Diameter observation areal | 21 mm |
| Height with / without lid | 14 mm / 12 mm |
| Bottom: Glass coverslip No. 1.5H, selected quality, 170 µm +/- 5 µm | |
Geometry of the Grid-500
| Number of squares | 400 |
| Repeat distance | 500 µm (+/- 1 %) |
| Groove width | 20 µm (± 5 µm) |
| Groove depth | < 5 µm |
Technical Features
- Grid with 500 µm repeat distance
- Lettered and numbered 4 x 100 squares (A-U; 1-20)
- Grid and cells in one focal plane
- Bottom made from D 263 M Schott glass with a thickness of 170 µm +/- 5 µm
- Lid with lock position, which minimizes evaporation
- May require coating to promote cell attachment
- Grid-500 also available with ibidi Polymer Coverslip bottom in µ-Dish 35 mm, high Grid-500 or µ-Slide 8 Well Grid-500
Layout Grid-500 Glass Bottom
Experimental Example
Triple immunofluorescence of MDCK cells
Red: mitochondria, stained with MitoTracker™ Red CMXRos
Green: F-actin, stained with Alexa Fluor™ 488 Phalloidin
Blue: nuclei, stained with DAPI
ibidi Polymer Coverslip vs. ibidi Glass Bottom
ibidi Polymer Coverslip | ibidi Glass Bottom | |
| Optical properties | ||
| Refractive index (nD 589 nm) | 1.52 | 1.52 |
| Abbe number | 56 | 55 |
| Thickness | #1.5 (180 µm) | #1.5H (170 µm) |
| Material | Microscopy plastic | D 263M Schott borosilicate glass |
| Autofluorescence | Low | Low |
| Transmission | Very high (even ultraviolet) | High (ultraviolet restrictions) |
| Birefringence (DIC) | Low (DIC compatible*) | Low (DIC compatible*) |
| Other aspects | ||
| Surface modifications | ibiTreat – tissue culture treated Uncoated – hydrophobic | Only glass |
| Protein coatings | Compatible | Compatible |
| Gas permeable | Yes | No |
| Material flexibility | High | Low |
| Breakable | No | Yes |
| Applications | Fluorescence microscopy | TIRF and single photon |
* Special glass DIC Lids are available separately
