จานเพาะเลี้ยงเซลล์ชนิดพิเศษ (Elastically Supported Surface, ESS) ขนาด 35 มม. – เหมาะสำหรับการจำลองการเลี้ยงเซลล์ภายใต้สภาวะ “in vivo-near”

• พื้นผิวมีความยืดหยุ่นจำลองการเลี้ยงเซลล์แบบ “in vivo-near”
• รองรับการถ่ายภาพความละเอียดสูงด้วยกล้องจุลทรรศน์
• ลักษณะของจานเพาะเลี้ยงเซลล์เหมือนกับ µ-Dish ^{35 mm, ขอบสูง}

A 35 mm imaging dish with an Elastically Supported Surface (ESS) used for growing cells under “in vivo-near” conditions

• “in vivo-near” cell culture on elastically supported surface
• High resolution microscopy on soft cell culture surface
• Based on the standard µ-Dish ^{35 mm, high}
• Pcs./Box: 30 (individually packed)
  Elastic Surface ESS: ESS 15 kPa
  Surface Modification: ESS: uncoated, hydrophobic, sterilized
• Pcs./Box: 30 (individually packed)
  Elastic Surface ESS: ESS 28 kPa
  Surface Modification: ESS: uncoated, hydrophobic, sterilized
• Pcs./Box: 30 (individually packed)
  Elastic Surface ESS: ESS Variety Pack
  Surface Modification: ESS: uncoated, hydrophobic, sterilized
• Pcs./Box: 30 (individually packed)
  Elastic Surface ESS: ESS 1.5 kPa
  Surface Modification: ESS: uncoated, hydrophobic, sterilized

Applications

• Cell culture under “in vivo-near” conditions, e.g. cardiomyocytes
• Cellular mechanics research
• Immunofluorescence stainings
• Live cell imaging of cell differentiation on soft surface
• Transfection
• Suitable for DIC , when used with the special DIC Lid

Specifications

Ø µ-Dish	35 mm
Volume	2 ml
Growth area	3.5 cm2
Coating area using 800 µl	4.1 cm2
Ø observation area	21 mm
Height with / without lid	14/12 mm
Bottom	Elastic surface (40 µm) on glass coverslip (100 µm)

Technical Features

• 40 μm thick PDMS (Polydimethylsiloxane) layer on top of a 100 μm thick glass cover
• Available stiffness: 1.5 kPa, 15 kPa, and 28 kPa
• Biocompatible soft cell culture material
• High walls with a standard height for easy handling
• Lid with lock position, which minimizes evaporation
• Rim for easy opening
• No autofluorescence
• Fully compatible with the ibidi Heating System

For a detailed explanation on the ESS technology please refer to the Special Surfaces section of our website.

Experimental Examples

Stop and smell the roses: Primary mouse neonatal cardiomyocytes plated on an ibidi μ-Dish ^{35 mm, high} ESS (28 kPa) coated with laminin. Sarcomeres are stained with antibodies raised against the Z-disc protein alpha actinin (red), laminin (green), and DAPI (blue). Cells were imaged at the Wohl Cellular Imaging Centre on a Nikon Eclipse Ti Inverted Spinning Disk Confocal Microscope using a 60x objective. Data by Matt Stroud, BHF Centre of Excellence, James Black Centre, King’s College London, United Kingdom.

Differentiated mouse fibroblasts cultured on the elastic surface of the μ-Dish ^{35 mm, high} ESS (28 kPa). Green: zyxin (Alexa 488-conjugated antibody); red: alpha-smooth muscle actin (Cy5 conjugated antibody). Courtesy R. Merkel, Jülich, Germany.