Chamber slide แบบ 4 ช่อง – ประกอบด้วย intermediate plate เหมาะสำหรับเทคนิคแสงแบบ Phase contrast และการถ่ายภาพความละเอียดสูง

A chambered coverslip with 4 wells and a special intermediate plate for excellent phase contrast and high-end fluorescence microscopy

Applications

Want to know if you should use a glass or a polymer bottom for your application? Find out here.


Phase contrast microscopy is the most commonly used, transmitted light technique in cell culture. When working with phase contrast microscopy, it is crucial to have the two phase rings adjusted to each other. In open wells, the meniscus at the air-water-interphase works like a lens that refracts the beam path. This miscalibrates the phase rings, leading to poor contrast in the microscopic image.

Working with the µ-Slide 4 Well Ph+ diminishes the meniscus, so that the whole optical system is aligned, no matter which position of the well is being imaged.

Specifications

Outer dimensions (w x l)25.5 x 75.5 mm²
Number of wells4
Dimensions of wells
(w x l x h)
21.6 x 11.4 x 9.3 mm³
Volume per well700 µl
Liquid height3.0 mm
Total height with / without lid10.8 / 9.5 mm
Growth area per well2.5 cm²
Coating area per well5.9 cm²
Bottomibidi Polymer Coverslip

Technical Features

Experimental Example

Meniscus-Free Phase Contrast Microscopy

µ-Slide 4 Wellµ-Slide 4 Well Ph+
Top View

The µ-Slide 4 Well Ph+ (Phase Contrast +) is designed for excellent optical quality, especially for cell culture when normal phase contrast microscopy is being used. Opposed to the classic µ-Slide 4 Well, the Ph+ version provides a special intermediate plate in the center of the well. This plate flattens the meniscus that disturbs the phase contrast effect in normal open wells. Openings near the corners provide access to the wells for easy filling and aspiration of liquids.

This innovative technique supports meniscus-free phase contrast microscopy in a very convenient manner.

Working with the µ-Slide 4 Well Ph+ diminishes the meniscus and increases the area of well contrasted cells

The illustration on the left shows the perturbing effect of a meniscus. Light is refracted on the air-water-interface, leading to poor contrast in microscopy. Only the small center part exhibits satisfying phase contrast.

Working with the µ-Slide 4 Well Ph+ diminishes the meniscus and increases the area of nicely contrasted cells. This nice contrast is due to the parallel beam path that is created by the plate.

Comparison Ph+ Well versus Standard Well: No Meniscus Effect in Ph+ Well

Standard Well

Poor Phase Contrast
Excellent Fluorescence Microscopy

µ-Slide 4 Well Ph+

Excellent Phase Contrast
Excellent Fluorescence Microscopy

Excellent Phase Contrast in the µ-Slide 4 Well Ph+