Chamber slide แบบ 2 ช่อง ก้นแก้ว เหมาะสำหรับการใช้กล้องจุลทรรศน์แบบ TIRF
- การถ่ายภาพที่ยอดเยี่ยมเนื่องจากก้นภาชนะทำมาจากแก้วที่มีความบางเท่ากับ coverslip
- ประหยัดค่าใช้จ่ายในการทำการทดลองเนื่องจาก สามารถใช้เซลล์จำนวนน้อย และ reagent ปริมาณน้อย
A chambered coverslip with 2 wells and a #1.5H glass bottom, suitable for use in TIRF and single molecule applications
- A cell culture chamber for observation of the sample through the coverslip-like bottom using high resolution microscopy
- A microscopy slide for brilliant cell imaging thanks to the low thickness variability of the coverslips’ glass
- Cost-effective experiments using small numbers of cells and low volumes of reagents
- Surface Modification: #1.5H (170 µm +/- 5 µm) D 263 M Schott glass, sterilized
Pcs./Box: 15 (individually packed)
Applications
- Cultivation and microscopy of cell cultures
- TIRF and single molecule applications
Specifications
| Outer dimensions (w x l) | 25.5 x 75.5 mm² |
| Number of wells | 2 |
| Dimensions of wells (w x l x h) | 21.6 x 23.8 x 9.3 mm³ |
| Volume per well | 1500 µl |
| Total height with / without lid | 10.8 / 9.5 mm |
| Growth area per well | 5.1 cm² |
| Coating area per well | 7.5 cm² |
| Bottom: Glass coverslip No. 1.5H, selected quality, 170 µm +/- 5 µm | |
Technical Features
- Open µ-Slide with 2 independent wells
- Bottom made from D 263 M Schott glass, No. 1.5H
(170 +/- 5 µm) - May require coating to promote cell attachment
- Also available in a Ph+ version: intermediate plate in each well to avoid meniscus formation
- Also available as a µ-Slide 2 Well with an ibidi Polymer Coverslip Bottom for superior cell growth
ibidi Polymer Coverslip vs. ibidi Glass Bottom
ibidi Polymer Coverslip | ibidi Glass Bottom | |
| Optical properties | ||
| Refractive index (nD 589 nm) | 1.52 | 1.52 |
| Abbe number | 56 | 55 |
| Thickness | #1.5 (180 µm) | #1.5H (170 µm) |
| Material | Microscopy plastic | D 263M Schott borosilicate glass |
| Autofluorescence | Low | Low |
| Transmission | Very high (even ultraviolet) | High (ultraviolet restrictions) |
| Birefringence (DIC) | Low (DIC compatible*) | Low (DIC compatible*) |
| Other aspects | ||
| Surface modifications | ibiTreat – tissue culture treated Uncoated – hydrophobic | Only glass |
| Protein coatings | Compatible | Compatible |
| Gas permeable | Yes | No |
| Material flexibility | High | Low |
| Breakable | No | Yes |
| Applications | Fluorescence microscopy | TIRF and single photon |
