ibidi : µ-Dish 35 mm, low Grid-500

ibidi - 80156

µ-Dish 35 mm, low Grid-500 - จานเพาะเลี้ยงเซลล์ สำหรับการถ่ายภาพ แบบมี Grid (ขอบต่ำ)



จานเพาะเลี้ยงเซลล์แบบก้นบางพอลิเมอร์พิเศษ ขนาด 35 มม มี Grid ขนาด 500 µm (ขอบต่ำ) – เหมาะสำหรับการถ่ายภาพ และหาตำแหน่งของเซลล์

  • เหมาะสำหรับการหาตำแหน่งเซลล์หรือกลุ่มเซลล์
  • ก้นภาชนะทำมาจากพอลิเมอร์พิเศษของ ibidi มีความบางเท่ากับ coverslip
  • มีเทคโนโลยี ibiTreat surface เพื่อการเจริญเติบโตของเซลล์ในอุดมคติ
  • มีฝาปิด พร้อมตำแหน่งล็อคที่ขอบจาน ช่วยลดการระเหย
  • เหมาะสำหรับเทคนิค DIC เมื่อใช้ร่วมกับฝาปิดพิเศษสำหรับเทคนิค DIC

A 35 mm imaging dish with an ibidi Polymer Coverslip bottom, low walls, and an imprinted 500 µm cell location grid

  • Ideal for locating cells or cell clusters
  • Unique ibidi Polymer Coverslip combining
    • Excellent cell culture conditions; and
    • Supreme optical quality for high resolution microscopy
  • Ideal cell growth conditions provided by the ibiTreat surface
  • Lid with lock position, which minimizes evaporation
  • Suitable for DIC, when used with the special DIC Lid
  • Surface Modification: ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized
    Pcs./Box: 60 (individually packed)
    Grid: Grid-500


  • Relocating cells or cell clusters, e.g., transfected cells for clone picking
  • Counting events per defined area (e.g., for calculating transfection efficiency)
  • Providing a reference structure for cell movements
  • Treatment of distinct single cells, such as in microinjection
  • Following axon growth on a defined scale bar

Want to know if you should use a glass or a polymer bottom for your application? Find out here.


Grid repeat distance500 µm (+/- 1 %)
Ø µ-Dish35 mm
Volume0.8 ml
Growth area3.5 cm2
Coating area using 400 µl4.1 cm2
Ø observation area21 mm
Height with lid9 mm
Bottom: ibidi Polymer Coverslip

Technical Features

  • Standard format dish with a 35 mm diameter
  • Grid with a 500 µm repeat distance
  • Lettered and numbered fields (A-U; 1-20)
  • Also available with a glass bottom: µ-Slide 35 mm, high glass bottom with Grid-50 or with Grid-500
  • Ideal cell growth conditions provided by the ibiTreat tissue culture treated surface
  • Made of biocompatible material using no glues, consequently no cells are harmed
  • Lid with lock position, which minimizes evaporation
  • Grid-500 also available in a µ-Slide 8 Well version

Layout Grid-500

Lid with Locking Feature for Minimized Evaporation

All ibidi µ-Dishes are equipped with the special lid locking feature. The locking position minimizes evaporation and thereby provides excellent conditions for long-term studies in a non-humidified environment. Gas exchange (carbon dioxide, or oxygen) during cell culture is maintained thanks to the gas-permeable plastic material of the dish.

TIP: Use the locking feature only if minimal evaporation is required (e.g., outside incubators, non-humidified microscopy stages, etc.).

Experimental Examples

Fluorescence microscopy image of murine cerebellar Purkinje neurons cultured in an ibidi µ-Dish 35 mm, low Grid-500.

Fluorescence microscopy image of murine cerebellar Purkinje neurons cultured in an ibidi µ-Dish 35 mm, low Grid-500. The cells were stained against the IP3 receptor (magenta) and imaged using a Zeiss LSM800 confocal microscope with a 40x objective lens. Image by Masahiko Tanaka, Atsushi Nakano, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, Japan.

ibidi µ-Dish Family: Versions and Variations

µ-Dish 35 mm, high

High walls:
Used for all standard applications

µ-Dish 35 mm, low

Low walls:
Provide greater cell access (useful for micromanipulation)

ibidi Polymer Coverslip vs. ibidi Glass Bottom

ibidi Polymer Coverslipibidi Glass Bottom
Optical properties
Refractive index (nD 589 nm)1.521.52
Abbe number5655
Thickness#1.5 (180 µm)#1.5H (170 µm)
MaterialMicroscopy plasticD 263M Schott borosilicate glass
TransmissionVery high (even ultraviolet)High (ultraviolet restrictions)
Birefringence (DIC)Low (DIC compatible*)Low (DIC compatible*)
Other aspects
Surface modificationsibiTreat – tissue culture treated
Uncoated – hydrophobic
Only glass
Protein coatingsCompatibleCompatible
Gas permeableYesNo
Material flexibilityHighLow
ApplicationsFluorescence microscopyTIRF and single photon

* Special glass DIC Lids are available separately