จานเพาะเลี้ยงเซลล์แบบก้นแก้ว สำหรับการถ่ายภาพ ขนาด 35 มม. – เหมาะสำหรับกล้องจุลทรรศน์ชนิด TIRF

A 35 mm imaging dish with a glass bottom for use in TIRF, single molecule and super-resolution microscopy applications

Applications

Want to know if you should use a glass or a polymer bottom for your application? Find out here.

Specifications

Ø µ-Dish35 mm
Volume2 ml
Growth area3.5 cm2
Coating area using 400 µl4.1 cm2
Ø observation area21 mm
Height with / without lid14/12 mm
Bottom: Glass Coverslip No. 1.5H, selected quality, 170 µm +/- 5 µm

Technical Features

Test our everyday solution for cost-effective high-throughput experiments: Glass Bottom Dish 35 mm

The Principle of the µ-Dish 35 mm, high Glass Bottom

The Coverslip Bottom

The µ-Dish 35 mm, high Glass Bottom comes with a thin #1.5H Glass Coverslip Bottom made from D 263 M Schott borosilicate glass that has the highest optical quality. ibidi developed these glass surfaces specifically for TIRFsuper-resolution microscopy, and single molecule microscopy. The dish is also available with an #1.5 ibidi Polymer Coverslip Bottom for optimal cell adhesion and high-resolution microscopy in a variety of assays.

As another option for cost-effective high-throughput experiments, we offer the Glass Bottom Dish 35 mm with a #1.5 glass coverslip bottom.

Learn more about the coverslip bottom or the different surfaces of the ibidi chambers.

Find a comparison of the different ibidi Dishes 35 mm here.

Lid with Locking Feature for Minimized Evaporation

All ibidi µ-Dishes are equipped with the special lid-locking feature. The locking position minimizes evaporation, and thereby provides excellent conditions for long-term studies in a non-humidified environment. Gas exchange (carbon dioxide or oxygen) during cell culture is maintained thanks to the gas-permeable plastic material of the dish.

TIP: Use the locking feature if minimal evaporation is required (e.g., outside incubators, non-humidified microscopy stages, etc.).

Experimental Examples

Time lapse of a B16-F1 mouse melanoma cell migrating on the laminin-coated surface of the µ-Dish 35 mm, high Glass Bottom. Phase contrast microscopy images were acquired over a a period of 10 minutes in the ibidi Stage Top Incubator Slide/Dish, CO2 – Silver Line using a 100x immersion objective on an Olympus X-81 microscope.
Data by Jonas Scholz and Prof. Jan Faix, Hannover Medical School, Germany.

Time lapse TIRF imaging showing focal adhesion dynamics of a mutant NIH-3T3 mouse embryonic fibroblast cell, which was transfected with EGFP-Paxillin, in a µ-Dish 35 mm, high Glass Bottom. TIRF microscopy images were acquired over a period of 12 hours in the ibidi Stage Top Incubator Slide/Dish, CO2 – Silver Line using a TIRF 100x immersion objective on a Nikon Eclipse microscope.
Data by Jonas Scholz and Prof. Jan Faix, Hannover Medical School, Germany.

Surface-near F-actin network of a Dictyostelium discoideum DdLimE-GFP cell. Live cell imaging on a Glass Coverslip #1.5H using Total Interference Reflection Fluorescence (TIRF) microscopy.

Fluorescence microscopy of fixed rat fibroblasts on a Glass Coverslip #1.5H. F-actin filaments are stained with phalloidin (green), nuclei are stained with DAPI (blue).

Electron microscopy of longitudinal ultra-thin sections of mouse embryonic fibroblasts (MEFs). Before fixation and processing, the cells were cultivated on an ibidi μ-Dish 35 mm, high Glass Bottom. STEM imaging was done using a FEI Verios 460L. Structures are indicated by color overlays: lysosomes (red), mitochondria (cyan), ER (yellow), golgi (orange), microtubules (magenta), plasma membrane (green), nucleus (blue), actin (turquoise). Image by Christian Lamberz, University of Bonn, German Centre for Neurodegenerative Diseases (DZNE), Germany.

Comparison of the ibidi Dishes 35 mm

Please find a detailed comparison of the material specifications, including suitable microscopy applications for the ibidi Polymer Coverslip, the ibidi Glass Bottom, and further materials here.

µ-Dish 35 mm, highµ-Dish 35 mm, high Glass BottomGlass Bottom Dish 35 mm
Bottom material#1.5 ibidi Polymer Coverslip#1.5H ibidi Glass Coverslip#1.5 glass coverslip
Bottom thickness180 µm (+10/-5 µm)170 µm (+/-5 µm)170 µm (+20/-10 µm)
Bottom: gas permeabilityYesNoNo
Available surfacesibiTreat (tissue culture treated),
Uncoated (hydrophobic)
Uncoated glassUncoated glass
LidLid with locking featureLid with locking featureStandard lid
PackagingSterile, individually packedSterile, individually packedSterile, 10 pcs. per case
Quantity60 or 400 pcs./box60 or 400 pcs./box200 or 800 pcs./box
Low wall version available?Yes: µ-Dish 35 mm, lowYes: µ-Dish 35 mm, low Glass BottomNo
Gridded version available?Yes: µ-Dish 35 mm, high Grid-500Yes: µ-Dish 35 mm, high Grid-50 Glass Bottomµ-Dish 35 mm, high Grid-500 Glass BottomNo