กล้องจุลทรรศน์ชนิดคอนโฟคอล ความละเอียดสูงเทคนิค FAST Lifetime contrast ยี่ห้อ ไลก้า รุ่น Stellaris 8 Falcon FLIM

เป็นกล้องจุลทรรศน์คอลโฟคอลรุ่นใหม่ล่าสุด จากไลก้า ประเทศเยอรมันนี ชนิด FALCON ย่อมาจาก FAST Lifetime Contrast  เป็นเทคโนโลยีแห่งอนาคตในการถ่ายภาพเชิงฟังก์ชั่น โดยใช้พลังของอายุการเรืองแสงเพื่อตรวจสอบสรีรวิทยาของเซลล์ และสำรวจพลวัตในเซลล์ที่มีชีวิต โดยที่ Leica Stellaris 8 Falcon เป็นโซลูชั่นแบบครบวงจรสำหรับ Fluorescence Lifetime Imaging หรือ FLIM และช่วยให้สามารถถ่ายภาพตลอดอายุการใช้งานด้วยอัตราความเร็วแบบวีดีโอสำหรับการศึกษาจลนศาสตร์อย่างรวดเร็วในเซลล์ที่มีชีวิต

Leica Stellaris 8 Falcon ช่วยเพิ่มมิติใหม่ของคอนทราสต์ภาพของการถ่ายภาพตัวอย่างของคุณ โดยตรวจจับทางชีวิภาพและติดตามปฏิกิริยาระหว่างโปรตีน ขณะนี้ ข้อมูลแบบ FLIM สามารถใช้งานร่วมกับทุกรูปแบบของระบบ Stellaris 8 แล้ว

คุณสมบัติพิเศษ

  1. ติดตามปฏิกิริยาของโมเลกุลอย่างรวดเร็วผ่าน FLIM-FRET (Förster Resonance Energy Transfer) ใช้ไบโอเซนเซอร์เพื่อตรวจจับการเปลี่ยนแปลงในสถานะการเผาผลาญและสภาพแวดล้อมขนาดเล็ก
  2. ใช้คอนทราสต์ตลอดอายุการใช้งานเพื่อแยกฟลูออโรฟอร์หลายตัวออกจากกัน และสามารถแปลงข้อมูล FLIM ด้วยการฝึกอบรมขั้นต่ำก็สามรถใช้งานได้เองอย่างง่ายได้

STELLARIS 8 FALCON (FAst Lifetime CONtrast) is the future of functional imaging. Harness the power of fluorescence lifetime to investigate cellular physiology and explore dynamics in living cells. STELLARIS 8 FALCON is a fully integrated solution for Fluorescence Lifetime Imaging (FLIM) and enables video-rate lifetime imaging acquisition for rapid kinetic studies in live cells.

STELLARIS 8 FALCON adds a new dimension of contrast to your imaging, opening the door to biosensing and tracking of interactions between proteins. FLIM information is now available for all modalities of the STELLARIS 8 system.

Now you can:

Histological section from cat eye. Simultaneous spectral (grey) and FLIM (color) confocal imaging reveals contrast by lifetime. Acquisition and visualization using STELLARIS 8 FALCON and LAS X software.

Follow molecular interactions with FLIM-FRET

Modern research investigates how molecules interact to accomplish vital tasks. FLIM-FRET is the gold standard to explore these interactions.

STELLARIS 8 FALCON sets a new speed standard for FLIM instruments.

It enables FRET in highly dynamic cellular events. You can acquire and interpret FRET data in your daily experiments.

Monitor subtle and fast changes with biosensors

Biosensors are powerful reporters for metabolic activity, signaling mechanisms, pH and microenvironment changes.

STELLARIS 8 FALCON provides access to information contained in fluorescence lifetime, even for ultrafast events such as membrane potential dynamics. This information complements the spectral fluorescent intensity imaging and TauSense modes delivered by the STELLARIS 8 system.

More reliable and sensitive metabolic imaging

Autofluorescence can be an issue in conventional imaging. STELLARIS 8 FALCON turns it into valuable information. You can now transform autofluorescence into a reporter for metabolic status, cell differentiation, and cancer development.

Moreover, STELLARIS 8 FALCON enables imaging contrast in living tissue, where fluorescence labeling is often unspecific or destroys physiological conditions.

Autofluorescence in mammalian cells at non-physiological conditions (pH 8.5). The signal correlates with changes in the NAD/NADH endogenous pool. The development of oxidative stress reads out as decrease of fluorescence lifetime over time. Original image size: 512 x 512 pixels. Color bar scale (lifetime): ns.
Autofluorescence in mammalian cells at non-physiological conditions (pH 8.5). The signal correlates with changes in the NAD/NADH endogenous pool. The development of oxidative stress reads out as decrease of fluorescence lifetime over time. Original image size: 512 x 512 pixels. Color bar scale (lifetime): ns.

Fluorophore separation beyond the spectral options

Fluorescence labeling is the standard way to differentiate intracellular structures. Spectral separation is very powerful, but sometimes limited when the emission spectra are too close.

With STELLARIS 8 FALCON, you can exploit the full potential of fluorescence lifetime imaging to separate multiple fluorescent probes using exponential fitting, pattern fitting and the new FLIM phasor separation analysis.

Interactive image: Cytoskeleton structures distinguished by lifetime contrast. Vimentin immunolabeled with Alexa Fluor 555 (green), and tubulin immunolabeled with Alexa Fluor 546 (blue). The fluorophores are spectrally very similar, but they are separated using the fluorescence lifetime information. Image size: 512 x 512 pixels.

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FLIM

STELLARIS 8 FALCON for fast lifetime imaging

The STELLARIS 8 FALCON microscope overcomes the speed limitation of FLIM and opens the door to fast lifetime data.

Up to now, functional information extracted from fluorescence lifetime data of fast processes was hard to get due to the technical constraints of FLIM. FLIM acquisition speed was at least 10 times slower than recording the confocal intensity.

Using STELLARIS 8 FALCON fast lifetime contrast imaging, you can follow dynamic processes in cells at the appropriate pace. These tasks are possible thanks to a new way to measure time using TCSPC (Time-Correlated Single Photon Counting) together with intelligent algorithms for data handling and analysis.

Application note in Nature: SP8 FALCON: a novel concept in fluorescence lifetime imaging enabling video-rate confocal FLIM

Single channel image of fluorescent beads (magenta) in a solution containing Alexa Fluor 555 (green). Fluorophore separation based on fluorescence lifetime is possible at various speeds, for example 16 fps (top), 27 fps (video-rate, middle), and 83 fps (ultra-fast, bottom). Using lifetime information for dye separation is significantly more advantageous than intensity (grayscale). The movies show pixel-by-pixel fitting of the lifetime components. Frame size: 512 x 64 pixels. Scale bar: 10 µm.

All-in-one multimodal imaging

Combining FLIM with other modalities was never as easy as with the STELLARIS 8 FALCON. Until now, researchers had to cope with complex wiring and cumbersome file transfer tasks. With STELLARIS 8 FALCON, you can integrate lifetime information into your standard confocal workflow.

STELLARIS 8 FALCON is fully integrated in the LAS X acquisition and analysis software. It can record FLIM in four spectral channels simultaneously and up to 10 channels sequentially. STELLARIS 8 FALCON gives you access to lifetime contrast in 3D-stacks, time-lapse sequences, and even large mosaic-tiling formats.

With LAS X NAVIGATOR, you can expand your viewing area up to 10,000 times, saving precious time in identifying your regions of interest and exploring your samples in a whole new way.

Straightforward acquisition of complex samples. High resolution mouse embryo mosaic image of 722 tiles containing 190 Megapixels. FLIM data fitted with four characteristic fluorescence lifetimes, color coded. Acquisition: 1:23 h. Analysis: 1:00 h
Straightforward acquisition of complex samples. High resolution mouse embryo mosaic image of 722 tiles containing 190 Megapixels. FLIM data fitted with four characteristic fluorescence lifetimes, color coded. Acquisition: 1:23 h. Analysis: 1:00 h

Straightforward lifetime definition with phasors

Analysis with STELLARIS 8 FALCON using FLIM phasors provides a 2D visualization of lifetime components. With FLIM phasors you can follow microenvironmental changes, select components to multiplex signal and determine FRET efficiency.

Cells labeled with Alexa555-Phalloidin and H2B mCherry. Separation performed using FLIM phasors. The phasor plot clearly shows two distributions. Courtesy: Dr. Martin Stöckl, Department of Biology, University Konstanz, Germany. 
Cells labeled with Alexa555-Phalloidin and H2B mCherry. Separation performed using FLIM phasors. The phasor plot clearly shows two distributions. Courtesy: Dr. Martin Stöckl, Department of Biology, University Konstanz, Germany. 

The results you need – with one click

The LAS X software enables FLIM with a click and the same philosophy as the routine spectral imaging.

Even if you use microscopy as a complementary technique, you can find the essentials and start imaging right away.

More specialized functions are accessible as workflows and allow automation for your convenience.

1-click philosophy to focus on your science: STELLARIS 8 FALCON controlled by the LAS X software
1-click philosophy to focus on your science: STELLARIS 8 FALCON controlled by the LAS X software