Model : sCMOS Micrscope Camera K5 – For routine fluorescence imaging กล้องถ่ายภาพฟลูออเรสเซนต์สำหรับกล้องจุลทรรศน์ ยี่ห้อ ไลก้า
Leica – sCMOS Microscope camera K5 เป็นกล้องสำหรับการถ่ายภาพเรืองแสง เหมาะอย่างยิ่งสำหรับการทดสอบภูมิคุ้มกันและการสร้างภาพการเพาะเลี้ยงเซลล์แบบ 3 มิติ
Leica – sCMOS Microscope camera K5 สามารถสร้างภาพตัวอย่างหลากหลายมีความยืดหยุ่นสูง และสามารถถ่ายภาพได้ความละเอียดสูงสุดที่ 4.2 ล้านพิกเซล และสามาาถสตรีมภาพด้วยความเร็ว 40 เฟรมต่อวินาที
Leica – sCMOS Microscope camera K5 ช่วยลด exposure time และ phototoxic effects ที่ 80% quantum efficiency อีกด้วย
ด้วยคุณสมบัติดังกล่าวกล้อง Leica – sCMOS Microscope camera K5 จึงเป็นกล้องถ่ายภาพสำหรับการแบ่งกลุ่มเหตุการณ์ตามเวลาจริง หรือ real time events สำหรับการปรับปรุงสัญญาณ signal to background ของกล้องถ่ายภาพ Leica Thunder Imager
The K5 is ideal for routine fluorescence imaging applications such as immunostaining assays and 3D Cell Culture imaging.
Image a wide variety of sample types with the flexibility and power of high 4.2 megapixel resolution and fast 40 fps imaging brought to you by the K5.
Your benefits
- Bring the flexibility and power of sCMOS technology into your lab and image the details of your experiment
- Efficiently segment crucial real time events
- Improve signal-to-background of your THUNDER Imager
Flexibility of imaging applications
To achieve the best imaging conditions, you can adjust the K5 camera flexible parameters to meet your sample needs:
- Image dynamic cellular events with up to 40 fps imaging
- Resolve detailed structures thanks to high 4.2 MP resolution
- Reduce exposure times and phototoxic effects with high 80% quantum efficiency.
Comparison slider:
Many commonplace CCD cameras have relatively low QE and high noise, which can reduce signal-to-noise and make it difficult to image structures of interest.
This image with DAPI-Nuclei (blue), Alexa488-Actin (green), MitoRed-mitochondria (red), and Alexa647-WGA (magenta) show the improvements that the Scientific CMOS technology in the K5 can give over CCD cameras.
Efficiently segment real time events
Get more biologically-relevant information with time lapse imaging thanks to the increased throughput powered by up to 40 fps observations.
Video:
Imaging cellular motility and protein dynamics in live cells requires high QE and fast frame rates. The K5‘s features make it an incredible camera for routine live cell imaging.
Here, U2OS cells expressing LifeAct-TagGFP2 were imaged for 150ms every 500ms for 5 minutes. Samples courtesy of ibidi GmbH, Gräfelfing, Germany.
Improve your images with better signal-to-background
Combine the K5‘s flexibility with the power of Computational Clearing.
Remove the haze from conventional widefield images accurately thanks to validated K5 and THUNDER Imager workflows.
Comparison Slider:
The K5 and THUNDER Imager 3D Assay enables clear identification of Alpha (GFP-green) and Beta (mCardinal-red) cells within a developing zebrafish pancreas. Imaged in the blue (Hoechst), green (GFP), and red (mCardinal) channels, this 150-image z-stack was completed with all channels within a minute.
Images courtesy of Radhan Ramadass and Yu Hsuan at the Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany.
For routine fluorescence imaging
| Detector type | sCMOS |
| Quantum Efficiency | ~80% @ 600 nm |
| Speed (full resolution) | 40 fps |
| Dynamic Range | 21400:1 up to 87 dB |
| Resolution | 2048 x 2048 |
| Pixel size | 6.5 μm x 6.5 μm |
| Trigger type | SMA Connections |
| Data interface | USB 3.1 |
| Non-linearity | <0.6 % |
| Cooling | Passive |
| Read noise | 2.1 e- (median) / 2.3 e- (rms) |
| Dark current | 15 e-/px/sec |
| Sensor size (diagonal) | 18.8 mm |
| Shutter mode | Rolling |
| Fullwell capacity | 45000 e- |
| MTF | 76.9 lp/mm |
| Power consumption | 4.5 W |
| Weight | 420 g |
| Operating Temperature | 10 °C – 40 °C |
| Operable humidity range | 10 % – 80 % (non-condensing) |
| Optical interface | C-mount |
| CE/FCC Certification | Yes |
