ibidi : Heating System, Universal Fit, for 1 Chamber – Blue Line

ibidi - 10918 and 10915

ibidi Heating System, Universal Fit, for 1 Chamber – Blue Line



Heating System for live cell imaging applications including high-resolution and confocal microscopy

  • Stage top incubator that fits most inverted microscopes that have a regular frame or holder for multiwell plates
  • Excellent illumination of the sample – no condensation due to heated lid, optimal magnetic sample stabilization, and suitable for DIC
  • Perfectly suited for live cell imaging applications – full incubator conditions on the microscope
  • Available with or without Heated Lid

Complete system includes:

ibidi Temperature Controller – Blue Line
Heated Plate for 1 Chamber
Heated Glass Lid, Universal Fit
Heating Insert for ibidi
and non-ibidi Slide Formats


Live cell imaging that requires incubator conditions on the microscope, for example:
– Angiogenesis
– Cell migration, proliferation, wound healing
– Cell culture under flow
– Confocal microscopy
– Anaerobic conditions or oxidative stress

Rat fibroblasts (cell line)

Find Out More

Please find more detailed information about the planning, conduction, and data analysis of live cell imaging assays here.

Download the whole “Live Cell Imaging” Application Guide as a PDF here.


Temperature Controller – Blue Line
Operating humidityMax. 80% relative humidity (RH)
Environmental operating temperature18–30°C/64–86°F (min 5°C/9°F less than set temperature)
Dimensions90 mm x 170 mm x 230 mm
Weight1720 g / 3.8 lbs
External power supplyAC 100–240 V, 50/60 Hz, 2 A
Heated Plate and Lid
Plate dimensions127.5 x 85.5 x 12 mm³
(fits standard multiwell holders)
Lid dimensions127.5 x 85.5 x 19 mm³
Height of the system25.5 mm
Weight of incubation chamber with insert330 g / 0.46 lbs
Gas inletFemale Luer connector with Luer Lock
Restrictions for objective lenses
Geometrical:Possible collision with large objectives near the edges, narrowing the field of view
Custom openingsOn request

Heating Stages for Live Cell Imaging – No Condensation

The independently controlled, heated glass lid of the ibidi Heating & Incubation System solves the problem of condensation in live cell imaging.

By heating the lid to a temperature higher than the plate, a vertical temperature gradient is created. This gradient and an active humidity control prevent the formation of condensation on the lid of the Petri dish. The temperature at the cells’ site is maintained at a constant 37 °C.

Stable and Homogeneous Temperature

Stable and consistent temperature distribution in the µ-Slide VI 0.4 (left) and the µ-Slide 8 Well (right) during incubation in the ibidi Heating System, Universal Fit. Images were acquired with a FLIR thermal camera.

Components of the ibidi Heating & Incubation System, Universal Fit – Blue Line

ibidi Temperature Controller – Blue Line

The Temperature Controller controls the temperature of the different heated components (Heated Lid and Heated Plate). Four control channels are available.

Technical Features:

  • Temperature stability: ± 0.2 °C
  • Temperature uniformity across the insert: ± 0.5 °C (Heating Insert for slides and dishes)
  • Temperature range: Room temperature – 45 °C
  • Control channels: 4
  • Includes IncuControl software instrument control and data recording via a notebook
  • External temperature sensor included
  • Adjustable alarm function included
  • Analog output for extreme noise reduction optional

Heated Plate for 1 Chamber

  • Can be used with inverted microscopy systems
  • Fits into both manual and automated stages
  • Can be placed into regular multiwell frames on your microscope
  • Adaptable to all types of microscopy chambers, such as µ-Slides and µ-Dishes plus non-ibidi formats

Heated Plate for 4 µ-Slides

Heated Glass Lid, Universal Fit, for Use With CO2 and Humidity

  • For use with CO2, O2, and humidity to provide a complete environmental chamber
  • Independently controlled glass lid, warmed to 40 °C, prevents the formation of condensed water on the lid of the microscopy chamber or plate
  • No scattering of light or shadow formation

Heating Inserts

Optional: Heating Insert Adapter for Perfusion Assays

  • To be used with the ibidi Pump System for live cell imaging under flow conditions

Gas Mixer for the Incubation Unit

The heated lid also comes with a gas inlet. The ibidi Gas Incubation System upgrades the ibidi Heating System to a complete stage top incubator for all live cell imaging applications, including high resolution and confocal microscopy.

The ibidi Gas Incubation System is suitable for various experimental conditions (e.g., pH or hypoxia). It is available in two versions: Gas Mixer for CO2, Gas Mixer for CO2 and O2.

Live Cell Imaging Under Flow Conditions

The ibidi Heating & Incubation System can be adapted for live cell imaging under flow conditions. Connect the ibidi Pump System with the ibidi Heating System, Universal Fit, by using the Heating Insert Adapter for perfusion assays. This creates the perfect environment for long-term cell studies (several days) under flow conditions.

Overview of the Heating and Incubation System, Universal Fit – Blue Line

ibidi Heating System for Temperature Control of Multiwell Plates

ibidi has developed a Heating System for the use with K-Frame Stages or the Nikon Ti-S-E and Ti-S-ER Motorized Stage which hold multiwell plates. Thus, this system permits the user to control the temperature of samples held in multiwell plates.