Complete system includes:
ibidi Temperature Controller – Blue Line
Heated Plate for 1 Chamber
Heated Glass Lid, Universal Fit
Heating Insert for ibidi
and non-ibidi Slide Formats
Live cell imaging that requires incubator conditions on the microscope, for example:
– Cell migration, proliferation, wound healing
– Cell culture under flow
– Confocal microscopy
– Anaerobic conditions or oxidative stress
Rat fibroblasts (cell line)
Please find more detailed information about the planning, conduction, and data analysis of live cell imaging assays here.
Download the whole “Live Cell Imaging” Application Guide as a PDF here.
|Temperature Controller – Blue Line
|Max. 80% relative humidity (RH)
|Environmental operating temperature
|18–30°C/64–86°F (min 5°C/9°F less than set temperature)
|90 mm x 170 mm x 230 mm
|1720 g / 3.8 lbs
|External power supply
|AC 100–240 V, 50/60 Hz, 2 A
|Heated Plate and Lid
|127.5 x 85.5 x 12 mm³
(fits standard multiwell holders)
|127.5 x 85.5 x 19 mm³
|Height of the system
|Weight of incubation chamber with insert
|330 g / 0.46 lbs
|Female Luer connector with Luer Lock
|Restrictions for objective lenses
|Possible collision with large objectives near the edges, narrowing the field of view
The independently controlled, heated glass lid of the ibidi Heating & Incubation System solves the problem of condensation in live cell imaging.
By heating the lid to a temperature higher than the plate, a vertical temperature gradient is created. This gradient and an active humidity control prevent the formation of condensation on the lid of the Petri dish. The temperature at the cells’ site is maintained at a constant 37 °C.
Stable and consistent temperature distribution in the µ-Slide VI 0.4 (left) and the µ-Slide 8 Well (right) during incubation in the ibidi Heating System, Universal Fit. Images were acquired with a FLIR thermal camera.
The Temperature Controller controls the temperature of the different heated components (Heated Lid and Heated Plate). Four control channels are available.
The heated lid also comes with a gas inlet. The ibidi Gas Incubation System upgrades the ibidi Heating System to a complete stage top incubator for all live cell imaging applications, including high resolution and confocal microscopy.
The ibidi Gas Incubation System is suitable for various experimental conditions (e.g., pH or hypoxia). It is available in two versions: Gas Mixer for CO2, Gas Mixer for CO2 and O2.
The ibidi Heating & Incubation System can be adapted for live cell imaging under flow conditions. Connect the ibidi Pump System with the ibidi Heating System, Universal Fit, by using the Heating Insert Adapter for perfusion assays. This creates the perfect environment for long-term cell studies (several days) under flow conditions.
ibidi has developed a Heating System for the use with K-Frame Stages or the Nikon Ti-S-E and Ti-S-ER Motorized Stage which hold multiwell plates. Thus, this system permits the user to control the temperature of samples held in multiwell plates.