ibidi : µ-Slide 8 Well Glass Bottom

ibidi - 80827-90, 80827

ibidi : µ-Slide 8 Well Glass Bottom



A chambered coverslip with 8 wells and a #1.5H glass bottom, suitable for use in TIRF and single molecule applications

  • A cell culture chamber for observation of the sample through the coverslip-like bottom using high resolution microscopy
  • A microscopy slide for brilliant cell imaging thanks to the low thickness variability of the coverslips’ glass
  • Cost-effective experiments using small numbers of cells and low volumes of reagents
  • Surface Modification: #1.5H (170 µm +/- 5 µm) D 263 M Schott glass, sterilized
    Pcs./Box: 15 (individually packed)
  • Surface Modification: #1.5H (170 µm +/- 5 µm) D 263 M Schott glass, sterilized
    Pcs./Box: 90 (individually packed)


Want to know if you should use a glass or a polymer bottom for your application? Find out here.


Outer dimensions (w x l)25.5 x 75.5 mm²
Number of wells8
Dimensions of wells
(w x l x h)
9.4 x 10.7 x 6.8 mm³
Volume per well300 µl
Total height with lid8 mm
Growth area per well1.0 cm²
Coating area per well2.20 cm²
Bottom: Glass coverslip No. 1.5H, selected quality, 170 µm +/- 5 µm

Technical Features

  • Open µ-Slide with 8 independent wells
  • Bottom made from D 263 M Schott glass, No. 1.5H (170 +/- 5 µm)
  • May require coating to promote cell attachment
  • Available as a Bulk Pack with 90 individually packed µ-Slides per box
  • Also available as a µ-Slide 8 Well with an ibidi Polymer Coverslip Bottom for superior cell growth
  • Now also available as a µ-Slide 8 Well high Glass Bottom with extra high individual walls to keep cross contamination between wells as low as possible

Experimental Example

Surface-near F-actin network of a Dictyostelium discoideum DdLimE-GFP cell. Live cell imaging on a Glass Coverslip #1.5H using Total Interference Reflection Fluorescence (TIRF) microscopy.

ibidi Polymer Coverslip vs. ibidi Glass Bottom

ibidi Polymer Coverslipibidi Glass Bottom
Optical properties
Refractive index (nD 589 nm)1.521.52
Abbe number5655
Thickness#1.5 (180 µm)#1.5H (170 µm)
MaterialMicroscopy plasticD 263M Schott borosilicate glass
TransmissionVery high (even ultraviolet)High (ultraviolet restrictions)
Birefringence (DIC)Low (DIC compatible*)Low (DIC compatible*)
Other aspects
Surface modificationsibiTreat – tissue culture treated
Uncoated – hydrophobic
Only glass
Protein coatingsCompatibleCompatible
Gas permeableYesNo
Material flexibilityHighLow
ApplicationsFluorescence microscopyTIRF and single photon