ไมโครเพลทจำนวน 384 หลุมสีดำชนิดก้นทำจากแก้ว

A black 384 well plate with square wells and a #1.5H glass coverslip bottom for high throughput screening using TIRF and super-resolution microscopy

Applications

Specifications

Length / width127.7 / 85.5 mm
Height with / without lid17.2 / 15.0 mm
Well-to-well distance4.5 mm
Well clearance0.73 mm
Single well dimensions3.4 x 3.4 mm²
Single well depth12.8 mm
Volume per well50 µl
Growth area per well0.11 cm²
Coating area per well using 50 µl0.8 cm²
Bottom: Glass Coverslip No. 1.5H, selected quality, 170 µm +/- 5 µm

Technical Drawing

Technical drawings and details are available in the Instructions (PDF).

Technical Features

The Principle of the µ-Plate 384 Well Black Glass Bottom

The Coverslip Bottom

The µ-Plate 384 Well Black Glass Bottom features optimal flatness properties, which are crucial for high-resolution microscopy in screening applications. The glass coverslip provides high accuracy and facilitates imaging from well to well across the entire plate with less variation in focus due to the rigid material with high mechanical stability.

Application Examples

Fluorescence microscopy of immunostained Rat1 fibroblasts in the µ-Plate 384 Well Black Glass Bottom. Cells were stained for F-actin (green), alpha-Tubulin (red) and nuclei (DAPI, blue) and imaged using a 60x objective.

Composition of 16 stitched fluorescence microscopy images showing one whole well of the µ-Plate 384 Well Black Glass Bottom. Rat1 cells were stained with DAPI to visualize the nuclei and imaged using a 10x objective. Overlay with brightfield (well contours).