ibidi : µ-Dish 35 mm, high Bioinert – จานเพาะเลี้ยงเซลล์ชนิด Bionert ขนาด 35 มม. ขอบสูง

จานเพาะเลี้ยงเซลล์ขนาด 35 มม. พื้นผิวภาชนะป้องกันการเกาะติด – เหมาะสำหรับตัวอย่างสเฟียรอยด์, ออร์แกนอยด์ และเซลล์ลอย

Long-term culture and high-resolution microscopy of spheroids, organoids, and suspension cells on a non-adherent surface

Applications

Technical Features

Specifications

Bioinert Surface

Bioinert Surface thickness200 nm
Bioinert Surface materialPolyol-based hydrogel
Protein coatingsNot possible

µ-Dish 35 mm, high

Ø µ-Dish35 mm
Volume2 ml
Growth area3.5 cm2
Ø observation area21 mm
Height with / without lid14/12 mm
Bottom: ibidi Polymer Coverslip with Bioinert surface

ibiTreat, Uncoated, and Bioinert—A Surface Comparison

ibiTreat

Excellent cell adhesion

The hydrophilic ibiTreat surface provides excellent cell adhesion, even without a defined protein coating. However, ECM protein coatings can be done on ibiTreat without any restrictions. The ibiTreat surface is ideal for culture of adherent cells that do not need any specific coating.

Uncoated

Weak cell adhesion

The hydrophobic Uncoated surface provides weak cell adhesion, if not previously coated with an ECM protein. ECM protein coatings can be done on the Uncoated surface without any restrictions. The Uncoated surface is ideal for the culture of adherent cells that require a specific coating.

Bioinert

No cell adhesion

The hydrophilic Bioinert surface hinders any protein attachment, thus inhibiting subsequent cell attachment. Unlike with the ibiTreat and Uncoated surfaces, a coating is not possible. The Bioinert surface is ideal for the culture of suspension cells and cell aggregates.

Application Example: Spheroid Culture and FDA/PI Staining Using the Bioinert Surface

The Bioinert surface is ideally suited for the short- and long-term 3D culture of spheroids and organoids. Since both specific and non-specific cell immobilization are inhibited, cells are forced into a suspended state, enabling formation of small and large spheroids. To assess the viability, the cells were stained using fluorescein diacetate (FDA), which marks viable cells, and propidium iodide (PI), which stains dead cells.

For more detailed information about FDA/PI staining, please refer to our Application Note 33, Live/Dead Staining With FDA and PI (PDF).

Confocal laser scanning section of a HepG2 spheroid. Spheroids of HepG2 cells were generated with an agarose-based liquid overlay technique and transferred to the µ-Dish 35 mm, high Bioinert. FDA (green) indicates living cells at the outer spheroid layers. PI (red) marks dead cells within the necrotic spheroid center. Microscope: Zeiss LSM 710, Plan Apochromat objective lens 10x/0.45. Scale bar is 200 µm.