ibidi : µ-Dish 35 mm, high – จานเพาะเลี้ยงเซลล์ สำหรับการถ่ายภาพ ขนาด 35 มม. (ขอบสูง)

จานเพาะเลี้ยงเซลล์ แบบก้นบางพิเศษ แบบ polymer coverslip สำหรับการถ่ายภาพ เส้นผ่านศูนย์กลางขนาด 35 มม. – ให้ภาพความละเอียดสูง เหมาะสำหรับยึดติด หรือ Adherence cell

มีเทคโนโลยี ibiTreat surface เพื่อการเจริญเติบโตของเซลล์ มีฝาปิด พร้อมตำแหน่งล็อคที่ขอบจาน ช่วยลดการระเหยเหมาะสำหรับเทคนิค DIC เมื่อใช้ร่วมกับฝาปิดพิเศษสำหรับเทคนิค DIC

A 35 mm imaging dish with a polymer coverslip bottom for high-end microscopy and cell-based assays

Applications

Want to know if you should use a glass or a polymer bottom for your application? Find out here.

Specifications

Ø µ-Dish35 mm
Volume2 ml
Growth area3.5 cm2
Coating area using 400 µl4.1 cm2
Ø observation area21 mm
Height with / without lid14/12 mm
Bottom: ibidi Polymer Coverslip

Technical Features

Test our everyday solution for cost-effective high-throughput experiments: Glass Bottom Dish 35 mm

The Principle of the µ-Dish 35 mm, high

The Coverslip Bottom

The µ-Dish 35 mm, high comes with a thin ibidi Polymer Coverslip Bottom that has the highest optical quality (comparable to glass) and is ideally suitable for high-resolution microscopy. It is also available with a #1.5H Glass Coverslip Bottom for TIRF or single molecule microscopy.

As another option for cost-effective high-throughput experiments, we offer the Glass Bottom Dish 35 mm with a #1.5 glass coverslip bottom.

Find more information and technical details about the coverslip bottom of the ibidi chambers here.
Find a comparison of the different ibidi Dishes 35 mm here.

The ibiTreat Surface

ibiTreat (tissue culture-treated) is our most recommended surface modification, because almost all adherent cells grow well on it without the need for any additional coating.

Find more information about the different surfaces of the ibidi chambers here.

Lid with Locking Feature for Minimized Evaporation

All ibidi µ-Dishes are equipped with the special lid-locking feature. The locking position minimizes evaporation, and thereby provides excellent conditions for long-term studies in a non-humidified environment. Gas exchange (carbon dioxide or oxygen) during cell culture is maintained thanks to the gas-permeable plastic material of the dish.

TIP: Use the locking feature if minimal evaporation is required (e.g., outside incubators, non-humidified microscopy stages, etc.).

Experimental Examples

Differentiated PC12 cells in a μ-Dish 35 mm, high, stained for ß-III-Tubulin (Covance, Princeton, USA) in red

Differentiated PC12 cells in a μ-Dish 35 mm, high, stained for ß-III-Tubulin (Covance, Princeton, USA) in red. PC12 cells were incubated with commercial green-fluorescent magnetic nanoparticles (MAG-ARA, Chemicell, Berlin) and nuclei were stained with 4‘, 6-diamidino-2- phenylindole (DAPI, Roche Applied Systems, Indianapolis, USA). Image by Josephine Pinkernelle, Otto-Von-Guericke University Magdeburg, Magdeburg, Germany.

Ultra-structure of Streptococcus pyogenes biofilm grown on mouse embryonic fibroblasts in a μ-Dish 35 mm, high ibiTreat.

Ultra-structure of Streptococcus pyogenes biofilm grown on mouse embryonic fibroblasts in a μ-Dish 35 mm, high ibiTreat. Blue – Phalloidin AF350; Green – anti-Streptococcus pyogenes primary antibody, Rabbit anti-goat AF488 secondary antibody; Red – WGA AF594. Image by Anuradha Vajjala, Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, Singapore.

Brightfield and fluorescence images of multicellular shapes created using Biopixlar

Brightfield and fluorescence images of multicellular shapes created in the μ-Dish 35 mm, high using Biopixlar, a bioprinter capable of generating multicellular biological tissues directly in the cell culture media. Images by Fluicell.

Live rat kidney cells stained with Di-8-ANEPPS to image primary cilia

Live rat kidney cells stained with Di-8-ANEPPS to image primary cilia. Cells were plated on a μ-Dish and imaged on a Nikon A1R inverted confocal microscope with a 60X NA 1.27 WI objective using 457nm excitation and a 490nm LP filter to the detection PMT. 3D volume rendering has been depth coded with NIS Elements software. Image by Brian Siroky, Alyssa Wilson, and Matthew Kofron, Cinncinnati Children’s Hospital, Cincinnati, OH, USA.

ibidi µ-Dish Height Variations

µ-Dish 35 mm, high

High walls for all standard cell culture applications


µ-Dish 35 mm, low

Low walls that provide greater cell access, which is useful for micromanipulation

Triple immunofluorescence of bovine endothelial cells.
Red: mitochondria, stained with MitoTracker™ Red CMXRos;
Green: F-actin, stained with Alexa Fluor™ 488 Phalloidin;
Blue: nuclei, stained with DAPI.

Comparison of the ibidi Dishes 35 mm

Please find a detailed comparison of the material specifications, including suitable microscopy applications for the ibidi Polymer Coverslip, the ibidi Glass Bottom, and further materials here.

µ-Dish 35 mm, highµ-Dish 35 mm, high Glass BottomGlass Bottom Dish 35 mm
Bottom material#1.5 ibidi Polymer Coverslip#1.5H ibidi Glass Coverslip#1.5 glass coverslip
Bottom thickness180 µm (+10/-5 µm)170 µm (+/-5 µm)170 µm (+20/-10 µm)
Bottom: gas permeabilityYesNoNo
Available surfacesibiTreat (tissue culture treated),
Uncoated (hydrophobic)
Uncoated glassUncoated glass
LidLid with locking featureLid with locking featureStandard lid
PackagingSterile, individually packedSterile, individually packedSterile, 10 pcs. per case
Quantity60 or 400 pcs./box60 or 400 pcs./box200 or 800 pcs./box
Low wall version available? Yes: µ-Dish 35 mm, lowYes: µ-Dish 35 mm, low Glass BottomNo
Gridded version available?Yes: µ-Dish 35 mm, high Grid-500Yes: µ-Dish 35 mm, high Grid-50 Glass Bottomµ-Dish 35 mm, high Grid-500 Glass BottomNo