จานเพาะเลี้ยงเซลล์แบบก้นแก้ว สำหรับการถ่ายภาพ ขนาด 35 มม. แบบมี grid ขนาด 500 ไมครอน เพื่อการหาตำแหน่งเซลล์

A 35 mm imaging dish with a glass bottom and an imprinted 500 µm cell location grid

Applications

Want to know if you should use a glass or a polymer bottom for your application? Find out here.

Specifications

Geometry of the µ–Dish 35 mm, high Glass Bottom

Diameter dish35 mm
Volume  2 ml
VGrowth area3.5 cm2
Coating area using 400 µl4.1 cm2
Diameter observation areal21 mm
Height with / without lid14 mm / 12 mm
Bottom: Glass coverslip No. 1.5H, selected quality, 170 µm +/- 5 µm


Geometry of the Grid-500

Number of squares400
Repeat distance500 µm (+/- 1 %)
Groove width20 µm (± 5 µm)
Groove depth< 5 µm

Technical Features

Layout Grid-500 Glass Bottom

Experimental Example

Triple immunofluorescence of MDCK cells
Red: mitochondria, stained with MitoTracker™ Red CMXRos
Green: F-actin, stained with Alexa Fluor™ 488 Phalloidin
Blue: nuclei, stained with DAPI

ibidi Polymer Coverslip vs. ibidi Glass Bottom

ibidi Polymer Coverslipibidi Glass Bottom
Optical properties
Refractive index (nD 589 nm)1.521.52
Abbe number5655
Thickness#1.5 (180 µm)#1.5H (170 µm)
MaterialMicroscopy plasticD 263M Schott borosilicate glass
AutofluorescenceLowLow
TransmissionVery high (even ultraviolet)High (ultraviolet restrictions)
Birefringence (DIC)Low (DIC compatible*)Low (DIC compatible*)
Other aspects
Surface modificationsibiTreat – tissue culture treated
Uncoated – hydrophobic
Only glass
Protein coatingsCompatibleCompatible
Gas permeableYesNo
Material flexibilityHighLow
BreakableNoYes
ApplicationsFluorescence microscopyTIRF and single photon

* Special glass DIC Lids are available separately