ibidi : µ-Dish 35 mm, high Grid-500 Glass Bottom

ibidi - 81168

µ-Dish 35 mm, high Grid-500 Glass Bottom - จานเพาะเลี้ยงเซลล์แบบก้นแก้ว ขนาด 35 มม แบบมี grid 500 ไมครอน



จานเพาะเลี้ยงเซลล์แบบก้นแก้ว สำหรับการถ่ายภาพ ขนาด 35 มม. แบบมี grid ขนาด 500 ไมครอน เพื่อการหาตำแหน่งเซลล์

  • ออกแบบมาสำหรับการหาตำแหน่งเซลล์
  • ง่ายต่อการนับเซลล์ต่อพื้นที่
  • ก้นภาชนะทำมาจากแก้วเหมาะสำหรับกล้องจุลทรรศน์แบบ TIRF

A 35 mm imaging dish with a glass bottom and an imprinted 500 µm cell location grid

  • Ideal for locating cells or cell clusters
  • Easy cell counting per defined area
  • Glass coverslip for TIRF and single molecule applications
  • Surface Modification: #1.5H (170 µm +/- 5 µm) D 263 M Schott glass, sterilized
    Pcs./Box: 30 (individually packed)
    Grid: Grid-500


  • Locating cells or cell clusters, e.g., transfected cells for clone picking
  • Counting events per defined area (e.g., when calculating transfection efficiency)
  • Providing a reference structure for cell movements
  • Treatment of distinct single cells, such as in microinjection
  • Following axon growth on a defined scale bar

Want to know if you should use a glass or a polymer bottom for your application? Find out here.


Geometry of the µ–Dish 35 mm, high Glass Bottom

Diameter dish35 mm
Volume  2 ml
VGrowth area3.5 cm2
Coating area using 400 µl4.1 cm2
Diameter observation areal21 mm
Height with / without lid14 mm / 12 mm
Bottom: Glass coverslip No. 1.5H, selected quality, 170 µm +/- 5 µm

Geometry of the Grid-500

Number of squares400
Repeat distance500 µm (+/- 1 %)
Groove width20 µm (± 5 µm)
Groove depth< 5 µm

Technical Features

  • Grid with 500 µm repeat distance
  • Lettered and numbered 4 x 100 squares (A-U; 1-20)
  • Grid and cells in one focal plane
  • Bottom made from D 263 M Schott glass with a thickness of 170 µm +/- 5 µm
  • Lid with lock position, which minimizes evaporation
  • May require coating to promote cell attachment
  • Grid-500 also available with ibidi Polymer Coverslip bottom in µ-Dish 35 mm, high Grid-500 or µ-Slide 8 Well Grid-500

Layout Grid-500 Glass Bottom

Experimental Example

Triple immunofluorescence of MDCK cells
Red: mitochondria, stained with MitoTracker™ Red CMXRos
Green: F-actin, stained with Alexa Fluor™ 488 Phalloidin
Blue: nuclei, stained with DAPI

ibidi Polymer Coverslip vs. ibidi Glass Bottom

ibidi Polymer Coverslipibidi Glass Bottom
Optical properties
Refractive index (nD 589 nm)1.521.52
Abbe number5655
Thickness#1.5 (180 µm)#1.5H (170 µm)
MaterialMicroscopy plasticD 263M Schott borosilicate glass
TransmissionVery high (even ultraviolet)High (ultraviolet restrictions)
Birefringence (DIC)Low (DIC compatible*)Low (DIC compatible*)
Other aspects
Surface modificationsibiTreat – tissue culture treated
Uncoated – hydrophobic
Only glass
Protein coatingsCompatibleCompatible
Gas permeableYesNo
Material flexibilityHighLow
ApplicationsFluorescence microscopyTIRF and single photon

* Special glass DIC Lids are available separately