จานเพาะเลี้ยงเซลล์แบบก้นบางพอลิเมอร์พิเศษ ขนาด 35 มม มี Grid ขนาด 500 µm (ขอบสูง) – เหมาะสำหรับการถ่ายภาพ และหาตำแหน่งของเซลล์

A 35 mm imaging dish with an ibidi Polymer Coverslip bottom, high walls, and an imprinted 500 µm cell location grid

Applications

Want to know if you should use a glass or a polymer bottom for your application? Find out here.

Specifications

Grid repeat distance500 µm (+/- 1 %)
Ø µ-Dish35 mm
Volume2 ml
Growth area3.5 cm2
Coating area using 400 µl4.1 cm2
Ø observation area21 mm
Height with lid14 mm
Bottom: ibidi Polymer Coverslip

Technical Features

Layout Grid-500

Lid with Locking Feature for Minimized Evaporation

All ibidi µ-Dishes are equipped with the special lid locking feature. The locking position minimizes evaporation and thereby provides excellent conditions for long-term studies in a non-humidified environment. Gas exchange (carbon dioxide, or oxygen) during cell culture is maintained thanks to the gas-permeable plastic material of the dish.

TIP: Use the locking feature only if minimal evaporation is required (e.g., outside incubators, non-humidified microscopy stages, etc.).

ibidi µ-Dish Family: Versions and Variations

µ-Dish 35 mm, high

High walls:
Used for all standard applications

µ-Dish 35 mm, low

Low walls:
Provide greater cell access (useful for micromanipulation)

ibidi Polymer Coverslip vs. ibidi Glass Bottom

ibidi Polymer Coverslipibidi Glass Bottom
Optical properties
Refractive index (nD 589 nm)1.521.52
Abbe number5655
Thickness#1.5 (180 µm)#1.5H (170 µm)
MaterialMicroscopy plasticD 263M Schott borosilicate glass
AutofluorescenceLowLow
TransmissionVery high (even ultraviolet)High (ultraviolet restrictions)
Birefringence (DIC)Low (DIC compatible*)Low (DIC compatible*)
Other aspects
Surface modificationsibiTreat – tissue culture treated
Uncoated – hydrophobic
Only glass
Protein coatingsCompatibleCompatible
Gas permeableYesNo
Material flexibilityHighLow
BreakableNoYes
ApplicationsFluorescence microscopyTIRF and single photon

* Special glass DIC Lids are available separately